STAG2基因在肾癌的表达及功能研究

发布时间：2018-05-05 20:50

本文选题：STAG + 肾肿瘤　；参考：《安徽医科大学学报》2018年01期

【摘要】：目的研究骨髓基质抗原2(STAG2)基因在人肾癌组织和肾癌细胞中的表达水平并研究STAG2基因对肾癌细胞生物学表型的影响。方法使用实时荧光定量PCR(qRT-PCR)检测STAG2基因在5株肾癌细胞、1株正常肾细胞和68对肾癌及其对应的癌旁组织中的表达水平;使用Western blot法分析STAG2蛋白在5株肾癌细胞、1株正常肾细胞的表达特点;使用慢病毒包装感染ACHN、786-O细胞并使用qRT-PCR检测感染效率;CCK8试剂盒检测STAG2过表达后对肾癌细胞增殖的影响;Transwell法检测STAG2过表达后对肾癌细胞侵袭的影响;流式细胞术检测STAG2过表达后对肾癌细胞凋亡的影响。结果 (1)qRT-PCR结果显示,与正常肾细胞相比,STAG2基因在5株肾癌细胞的表达显著下降,差异有统计学意义(P0.01);(2)Western blot结果显示在5株肾癌细胞中STAG2蛋白表达明显低于正常肾细胞;(3)qRT-PCR检测肾癌及其癌旁组织的STAG2表达水平结果显示与其对应癌旁组织相比,72.06%(49/68)肾癌组织中STAG2表达水平下降(P0.01);肾癌组织中STAG2表达水平与肿瘤的临床病理分期相关(P=0.029),与患者年龄、性别、肿瘤组织学分级无明显相关性;(4)慢病毒感染ACHN、786-O细胞后STAG2表达水平明显升高(P0.01);(5)体外实验中,过表达STAG2基因明显抑制肾癌细胞的增殖和迁移能力,促进肾癌细胞的早期凋亡(P0.05,P0.01)。结论 (1)在肾癌细胞和肾癌组织中STAG2的表达水平均有明显的下降,提示STAG2可能参与肾癌的发生和发展的过程,STAG2低表达对肾癌的早期诊断可能提供一些帮助;(2)上调STAG2基因的表达明显抑制肾癌细胞的增殖和迁移能力、促进肾癌细胞的凋亡,提示STAG2可能成为治疗肾癌的一个潜在靶点。
[Abstract]:Objective to study the expression of bone marrow stromal antigen 2STAG2) gene in human renal cell carcinoma (RCC) and the effect of STAG2 gene on the biological phenotype of RCC cells. Methods the expression of STAG2 gene in 5 normal renal cell lines and 68 pairs of renal cell carcinoma and its corresponding adjacent tissues were detected by real-time fluorescence quantitative PCRQ RT-PCR. Western blot assay was used to analyze the expression of STAG2 protein in 5 renal cell lines and 1 normal renal cell line. ACHN 786-O cells were infected with lentivirus packaging and the effect of STAG2 overexpression on the proliferation of renal cell carcinoma cells was detected by qRT-PCR kit. Transwell method was used to detect the effect of STAG2 overexpression on the invasion of renal cancer cells. The effect of overexpression of STAG2 on apoptosis of renal carcinoma cells was detected by flow cytometry. Results the results of QRT-PCR showed that the expression of statin G2 gene in 5 renal cancer cells was significantly decreased compared with that in normal renal cells. The expression of STAG2 protein in 5 renal cell lines was significantly lower than that in normal renal cell line and its adjacent tissues. The results showed that the expression level of STAG2 in renal cell carcinoma and its adjacent tissues was 72.06g / 4968%. The expression of STAG2 in renal cell carcinoma was correlated with the clinicopathologic stage of the tumor and the age of the patients. Sex and histological grade of tumor were not significantly correlated with the expression of STAG2 after lentivirus infection in ACHNN78-O cells. In vitro, overexpression of STAG2 gene significantly inhibited the proliferation and migration of renal cancer cells and promoted the early apoptosis of renal cancer cells. Conclusion the expression of STAG2 in RCC and RCC was significantly decreased. The results suggest that the low expression of STAG2 may be involved in the pathogenesis and development of renal cell carcinoma. The low expression of STAG2 may provide some help for the early diagnosis of renal carcinoma.) upregulating the expression of STAG2 gene can significantly inhibit the proliferation and migration of renal cancer cells and promote the apoptosis of renal cancer cells. The results suggest that STAG2 may be a potential target for the treatment of renal cell carcinoma.
【作者单位】：安徽医科大学深圳二院临床学院;深圳市第二人民医院泌尿外科;
【基金】：国家自然科学基金(编号:81503616) 中国博士后科学基金(编号:2016M600708) 深圳市科技计划项目(编号:JSGG20160301162913683、JCYJ20130329104732074) 深圳市高水平大学医学学科建设专项资金
【分类号】：R737.11

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