InDel用于中国人群个体识别的法医学研究

发布时间：2018-05-11 13:12

本文选题：法医遗传学 + 插入—缺失多态性　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:利用插入-缺失多态性(Insertion-Deletion Polymorphism,InDel)遗传标记,建立一套适用于中国人群个体识别鉴定的复合扩增体系,并对插入—缺失多态性遗传标记应用于法医DNA常见现场检材的检验进行研究。方法:通过对基因座的筛选,荧光染料的筛选,引物的设计调整,复合扩增体系的调配优化,反应条件的研究,构建复合扩增体系;从系统稳定性、系统灵敏度、种属特异性、组织同一性、各类案件检材及降解DNA检材的检测验证体系的实用性;最后利用本体系对汉族、苗族、哈萨克族、瑶族及傣族进行群体遗传学调查,进一步验证该体系个体识别效能。结果:在大量实验研究的基础上,成功地建立起1套包含30个InDel基因座和1个性别鉴定基因座,共计31个基因座的多重复合PCR扩增体系。该体系有较好的系统稳定性,对不同检材有很好的检验灵敏度,并且可以准确、高效的对微量及降解检材进行检测、分型。在群体遗传学调查中,汉族、苗族、哈萨克族、瑶族及傣族的累计个体识别率分别为 0.999999999957、0.999999999875、0.999999999990、0.999999999966、0.999999999974,与Qiagen公司的商品化试剂盒系统效能相当,与之相比,该体系所有扩增试剂、缓冲液,均为国产,降低了检测成本,基因座的选择更适用于中国人群,可以作为STR分型方法的有效补充工具。结论:该体系可用于中国人群的法医DNA个体识别,对不同检材有很好的检验灵敏度,对微量及降解检材具有很好的检测能力。
[Abstract]:Objective: to establish a multiplex amplification system for individual identification in Chinese population by using Insertion-Deletion Polymorphismm-InDell genetic markers. The application of insertion-deletion polymorphism genetic markers to the examination of common forensic DNA samples was studied. Methods: through the screening of loci, the screening of fluorescent dyes, the design and adjustment of primers, the optimization of multiplex amplification system and the study of reaction conditions, the multiplex amplification system was constructed from the aspects of system stability, system sensitivity and species specificity. The tissue identity, the practicability of the detection system for all kinds of cases and degraded DNA samples, and the population genetics investigation of Han, Miao, Kazak, Yao and Dai nationalities were carried out by this system. The effectiveness of the system is further verified. Results: based on a large number of experimental studies, a multiplex PCR amplification system consisting of 30 InDel loci and 1 sex-identifying locus was successfully established, with a total of 31 loci. The system has good system stability, good sensitivity to different samples, and can be accurately and efficiently for the detection and classification of trace and degradation samples. The cumulative individual identification rates of Han, Miao, Kazak, Yao and Dai nationalities were 0.999999999957-0.999999999877V 0.999999999999990 (0.9999999999909 9966) 0.999999999999744.Compared with the commercial kit system of Qiagen Company, all the amplification reagents, buffers, etc. The locus selection is more suitable for Chinese population and can be used as an effective supplementary tool for STR typing. Conclusion: the system can be used for individual identification of forensic DNA in Chinese population. It has good sensitivity for different samples and good detection ability for trace and degradable samples.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919

【相似文献】