RBMS3与SFRP1的低表达与胃癌患者预后不良相关

发布时间：2018-05-26 02:11

本文选题：胃癌 + RBMS3　；参考：《安徽医科大学》2017年硕士论文

【摘要】：背景:RNA结合基序,单链相互作用蛋白3(RNA binding motif,single stranded interacting protein 3,RBMS3)是Myc单链作用结合蛋白(MSSPs)家族中的一员,其可编码RNA结合蛋白,家族成员依次为RBMS1-3。研究表明RBMS3在少数鳞状细胞癌中发挥抑癌作用,而其在胃癌中的表达情况及作用机制仍不清楚。分泌型卷曲相关蛋白(Secreted frizzled-related protein,SFRP)家族拥有5个成员,依次为SFRP1-5。SFRP1作为Wnt/β-catenin通路拮抗剂,在多肿瘤中发挥抑癌作用。研究发现RBMS3与SFRP1均可通过下调c-Myc和β-catenin抑制肿瘤的发生发展。目的:检测人胃癌组织、对应癌旁正常组织中RBMS3及SFRP1的表达水平,明确RBMS3及SFRP1在胃癌中的表达情况,分别探索RBMS3及SFRP1的表达水平与胃癌的临床参数之间的关系,并分析在胃癌组织中RBMS3及SFRP1表达水平之间的相关性,及两者表达水平与胃癌患者预后的相关性,进而为进一步研究RBMS3及SFRP1在胃癌的发生发展中可能机制提供理论基础。方法:收集新鲜人胃癌组织(23例)、对应的癌旁组织(23例),另收集172例胃癌组织及43例癌旁组织制成组织芯片。应用实时荧光定量逆转录-聚合酶链反应(q RT-PCR)检测23对新鲜组织中RBMS3及SFRP1 m RNA的表达水平,同时应用蛋白免疫印迹(Western blot,WB)技术检测RBMS1及SFRP1的蛋白表达水平。进一步运用免疫组化(Immunohistochemical,IHC)检测172例胃癌组织及43例癌旁组织中RBMS3及SFRP1蛋白的表达水平。结果:胃癌组织、癌旁组织中RBMS3及SFRP1均有表达;RBMS3及SFRP1在胃癌组织中的表达水平明显均低于其在癌旁正常组织(P0.05),RBMS3与SFRP1在胃癌组织中的表达水平(m RNA和蛋白)呈正相关;RBMS3在胃癌中的蛋白表达水平与胃癌的分化程度、浸润深度显著相关,SFRP1在胃癌中的蛋白表达水平与胃癌的分化程度显著相关;RBMS3与SFRP1在胃癌中的蛋白表达水平均与胃癌患者预后相关,且两者共表达水平是胃癌患者的独立预后因子。结论:1、RBMS3及SFRP1在胃癌组织、癌旁组织中差异表达;2、RBMS3及SFRP1在胃癌组织中的表达水平呈正相关;3、RBMS3及SFRP1低表达的胃癌患者肿瘤分化程度差、预后差;4、RBMS3及SFRP1共表达水平可能是胃癌患者预后的一个有价值的生物学指标。
[Abstract]:Background: 3(RNA binding motifus single stranded interacting protein 3 (RBMS3) is a member of the Myc single-stranded binding protein (Myc) family, which encodes RNA binding proteins, and the family members are RBMS1-3. The results show that RBMS3 plays an inhibitory role in a small number of squamous cell carcinomas, but its expression and mechanism in gastric cancer are still unclear. There are five members of secreted frizzled-related protein family, SFRP1-5.SFRP1 as Wnt/ 尾 -catenin pathway antagonist, which plays a role of tumor suppressor in many tumors. It was found that both RBMS3 and SFRP1 inhibited tumor progression by down-regulating c-Myc and 尾-catenin. Objective: to detect the expression of RBMS3 and SFRP1 in human gastric cancer tissues, corresponding to the normal tissues adjacent to gastric cancer, to determine the expression of RBMS3 and SFRP1 in gastric cancer, and to explore the relationship between the expression of RBMS3 and SFRP1 and the clinical parameters of gastric cancer. The relationship between the expression of RBMS3 and SFRP1 in gastric cancer and the relationship between the expression of RBMS3 and SFRP1 and the prognosis of gastric cancer were analyzed, which provided a theoretical basis for the further study of the possible mechanism of RBMS3 and SFRP1 in the development of gastric cancer. Methods: 23 cases of fresh human gastric carcinoma tissues and 23 cases of adjacent tissues were collected. 172 cases of gastric cancer tissues and 43 cases of adjacent tissues were made into tissue microarray. The expression levels of RBMS3 and SFRP1 m RNA in 23 fresh tissues were detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression levels of RBMS1 and SFRP1 were detected by Western blotblotWB technique. The expression of RBMS3 and SFRP1 protein in 172 cases of gastric carcinoma and 43 cases of paracancerous tissues were detected by immunohistochemical staining (IHC). Results: gastric cancer tissue, The expression levels of RBMS3 and SFRP1 in gastric cancer tissues were significantly lower than those in adjacent normal tissues (P 0.05) and SFRP1 in gastric cancer tissues. There was a positive correlation between RBMS3 and SFRP1 in eggs of gastric cancer. The level of white expression and the degree of differentiation of gastric cancer, The expression of SFRP1 protein in gastric carcinoma was significantly correlated with the differentiation of gastric cancer. Both the expression of RBMS3 and SFRP1 in gastric carcinoma were correlated with the prognosis of gastric cancer. The coexpression level is an independent prognostic factor in patients with gastric cancer. Conclusion the differential expression of RBMS3 and SFRP1 in gastric cancer tissues and paracancerous tissues is positively correlated with the low expression of RBMS3 and SFRP1 in gastric cancer. The coexpression of RBMS3 and SFRP1 may be a valuable biological marker for the prognosis of gastric cancer patients.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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