通便汤对慢传输型便秘大鼠AQP3、AQP4的影响

发布时间：2018-05-29 12:15

本文选题：通便汤 + 慢传输便秘　；参考：《南京中医药大学》2017年硕士论文

【摘要】：目的:检测通便汤对水通道蛋白3(AQP3)、水通道蛋白4(AQP4)的影响,明确通便汤对AQP3、AQP4的调节作用,并探讨通便汤治疗慢传输便秘的作用机理。研究方法:将70只大鼠随机分为正常组(20只)与模型组(50只),正常组进食普通饲料,模型组进食含有复方地芬诺酯的饲料。模型复制成功后,处死正常组、模型组大鼠各10只,检测肠道推进程度及AQP3、AQP4表达及分布。剩余的10只正常组大鼠仍为正常组,剩余的40只模型组大鼠分为空白对照组、通便汤组、福松组、莫沙必利组,分别给予相应干预。运用炭墨推进法检测肠道传输功能,计数大鼠结肠内存留大便,检测结肠壁AQP3、AQP4的表达及在粘膜的分布及相对含量。结果:1模型复制成功后,与正常组比,模型组炭墨推进率低(P0.01)、存留大便多(P0.01);AQP3MOD值模型组高于正常组(p0.01);AQP4MOD值模型组高于正常组(p0.05)。2大鼠用药后,大便粒数及含水量:与空白对照组比,其余4组均比较高(p0.05);通便汤组与莫沙必利组差异不显著(p0.05),但高于福松组(p0.05)。炭墨推进率:与空白对照组比,通便汤组、莫沙必利组较高(p0.01),福松组差异不明显(p0.05)。结肠存留大便:通便汤组、福松组、莫沙必利组均与空白对照组有显著差异(p0.001)。3 IHC:AQP3主要分布在结肠粘膜顶部绒毛上皮细胞上,杯状细胞表达较少。着色主要是在细胞膜顶部、基底及腔面,其中顶部表达最多,空白对照组着色较深。空白对照组含量显著高于其余4组,其次为福松组,其含量高于通便汤组(p0.05)。AQP4主要在吸收细胞上表达,且以局灶表达为主,而在杯状细胞中无表达,黏膜顶部的表达高于底部,细胞基底面、腔面的表达高于侧壁。通便汤组与莫沙必利组无差异(p0.05),其余每两组之间均有显著差异(p0.01)。4Western-blot:空白对照组的AQP3、AQP4含量明显高于正常组(p0.001),通便汤组、福松组比空白对照组低(p0.05),莫沙必利组含量明显低于空白对照组(p0.01)。5荧光定量-pcr:AQP3:与空白对照组比,福松组与之接近(p0.05),莫沙必利组次之(p0.05),通便汤组最低(p0.01)。AQP4:与空白对照组比,通便汤组较低(p0.05),福松组、莫沙必利组均无显著差异(p0.05)。结论:1通便汤可明显改善便秘症状,通过下调AQP3、AQP4调节肠内水分吸收,治疗慢传输便秘效果良好。2本实验中,治疗慢传输便秘效果,通便汤最优,莫沙必利与之相近,福松次之。3通便汤与莫沙必利作用机制相似,但安全性更高,故临床上治疗慢传输便秘可能会更胜一筹。
[Abstract]:Objective: to investigate the effect of Tongbian decoction on aquaporin 3 aquaporin (AQP3) and aquaporin 4 (AQP4), to clarify the regulating effect of Tongbian decoction on AQP3 and AQP4, and to explore the mechanism of Tongbian decoction in the treatment of slow transit constipation. Methods: 70 rats were randomly divided into normal group (n = 20) and model group (n = 50). After the model was successfully copied, 10 rats in normal group and 10 rats in model group were killed. The intestinal propulsion degree and the expression and distribution of AQP3 AQP4 were detected. The remaining 10 normal rats were still normal, and the remaining 40 model groups were divided into blank control group, Tongbian decoction group, Fusong group and mosapride group, respectively. The intestinal transport function was detected by carbon ink propulsive method, and the colon defecation was counted. The expression of AQP3AAQP4 in colon wall and the distribution and relative content of AQP3AAQP4 in mucous membrane were detected. Results after the successful replication of the 1 / 1 model, compared with the normal group, the propelling rate of carbon ink in the model group was lower than that in the normal group (P 0.01), and the AQP3MOD value in the model group was higher than that in the normal control group (P 0.01) and AQP4MOD value in the model group was higher than that in the normal group (p 0.05.2), the number of feces and the water content in the model group were higher than those in the blank control group. There was no significant difference between Tongbian decoction group and mosapride group (P 0.05), but it was higher than that in Fusong group (P 0.05). The propelling rate of carbon and ink: compared with the blank control group, Tongbian decoction group and mosapride group were higher than that of the control group (P 0.01), but the difference of Fusong group was not significant (p 0.05). Colonic defecation: Tongbian decoction group, Fusapride group and mosapride group were significantly different from the blank control group in the distribution of p0.001t.3 IHC:AQP3 in the epithelial cells of the top of the colonic mucosa, and the expression of goblet cells was less. The staining was mainly on the top of the cell membrane, the basement and the surface of the cavity, the top of which was the most expressed, and the blank control group was deeply stained. The content of the blank control group was significantly higher than that of the other four groups, followed by the Fusong group, and its content was higher than that of the Tongbian decoction group, which was mainly expressed on the absorption cells and mainly on the focal cells, but not in the goblet cells, and the expression at the top of the mucous membrane was higher than that at the bottom. The expression of cell basal surface and cavity surface was higher than that of lateral wall. There was no difference between Tongbian decoction group and mosapride group, but there was significant difference between the other two groups. The content of AQP3 and AQP4 in the blank control group was significantly higher than that in the normal control group (P 0.001), and that in the Tongbian decoction group was significantly higher than that in the control group. Compared with the blank control group, the Fosong group was lower than the blank control group (P < 0.05), and the content of mosapride group was significantly lower than that of the blank control group (P 0.01). The fluorescence quantitative -pcr1: AQP3: compared with the blank control group, the Fusong group was close to the control group, the mosapride group was the second group, the lowest p0.01 .AQP4: compared with the blank control group. There was no significant difference between the Tongbian decoction group and the Fusapride group (P 0.05). Conclusion the constipation symptom can be obviously improved by using the decoction: through down-regulating AQP3AAQP4 to regulate the absorption of water in the intestine, the effect of treating slow transit constipation is good in this experiment, the effect of Tongbian decoction is the best, and mosapride is similar to the constipation. The mechanism of Fusong decoction is similar to that of mosapride, but the safety is higher, so the clinical treatment of slow transit constipation may be better.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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