白藜芦醇对大鼠脑缺血再灌注脑保护的剂量依赖的研究

发布时间：2018-06-13 07:32

本文选题：白藜芦醇 + 缺血/再灌注损伤　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:选择颈内动脉栓线法构建大脑中动脉阻塞(MCAO)脑缺血动物模型,分析注射各剂量白藜芦醇后对动物模型脑保护呈现出的剂量效应关系。方法:1.动物分组:75只SD大鼠,雄性,清洁级,体重均处于220~250g的范围内。随机将研究动物分成下列5组(15只/组):模型组、假手术组、低剂量(10 mg/kg)组、中剂量(30 mg/kg)组、高剂量(60mg/kg)组。构建局造性脑缺血/再灌注动物模型,观察海马神经元的形态学特征再灌注处理后第7天,通过水合氯醛(10%)对大鼠施麻后,从心脏处灌注磷酸盐缓冲液200ml(0.1M,4°C),再给予多聚甲醛200ml(4%),最终可观察到动物四肢僵硬,将大脑取出,置于固定液内保存至4°C温度下一整夜。取视交叉-大脑横裂区域,行石蜡固定包埋后,行均匀冠状切片,4μm/片,经常规脱蜡后,完成HE染色,于100倍显微镜下了解海马CA1区具体的神经元形态特征,并计算海马CA1区(1mm)受损锥体神经元的水平。结果:相比于假手术组,缺血再灌注常引起模型组动物具有更高的脑组织含水量;而相比于模型组,不同剂量组动物具有更低的脑组织含水量。光镜下观察海马神经元形态学特点结果显示:假手术组能够表现出完整的锥体神经元形态;而模型组能够观察到诸多异常特征,如变性、炎症及坏死等;注射中等剂量白藜芦醇能够在较大程度上缓解神经元受损情况;白藜芦醇、缺血均不会明显的影响齿回神经元的形态特征。结论:白藜芦醇能够有效的保护并改善局灶性缺血/再灌注受损情况,其中疗效最佳的为注射中等剂量的白藜芦醇组。
[Abstract]:Aim: to establish the animal model of middle cerebral artery occlusion (MCAO) with internal carotid artery occlusion and to analyze the dose-effect relationship of brain protection after different doses of resveratrol. Method 1: 1. The animals were divided into five groups: 75 SD rats, male, clean grade, weight in the range of 250 g. The study animals were randomly divided into the following five groups: model group, sham operation group, low dose 10 mg / kg group, middle dose 30 mg / kg group and high dose 60 mg / kg group. The morphological characteristics of hippocampal neurons were observed 7 days after reperfusion. The rats were anesthetized by chloral hydrate. Phosphate buffer (200ml) was perfused from the heart with a phosphate buffer (200ml) 0.1MU 4 掳C, and then was given paraformaldehyde (200ml). Finally, the stiff limbs of the animal were observed. The brain was removed and stored in the fixed solution for one night under 4 掳C temperature. The optic chiasma and the transverse fissure region of the brain were taken. After paraffin fixation, 4 渭 m / slice of uniform coronal section was performed. After dewaxing, HE staining was completed, and the specific neuronal morphological characteristics of hippocampal CA1 area were studied under 100 times microscope. The level of injured pyramidal neurons in hippocampal CA 1 area was calculated. Results: compared with sham operation group, ischemia reperfusion often caused higher water content of brain tissue in model group, and lower water content of brain tissue in different dose groups compared with model group. The morphological characteristics of hippocampal neurons were observed under light microscope: sham-operated group could show complete pyramidal neuron morphology, while model group could observe many abnormal features, such as degeneration, inflammation and necrosis. The moderate dose of resveratrol could alleviate the neuronal damage to a great extent, and neither resveratrol nor ischemia could significantly affect the morphological characteristics of dentate gyrus neurons. Conclusion: resveratrol can effectively protect and improve focal ischemia / reperfusion injury.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.3

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