薄皮木乙酸乙酯部位化学成分及抗炎活性的研究

发布时间：2018-06-17 02:39

本文选题：野丁香属 + 薄皮木　；参考：《郑州大学》2017年硕士论文

【摘要】：背景:野丁香属(Leptodermis Wall.)是茜草科(Rubiaceae)下的一个属,约40种,分布于喜马拉雅区至日本。根据《中国植物志》记载,在我国有35种,9变种,1变型,南北均有分布,但主产地为华北部、西南部部分省份。其中薄皮木(Leptodermis oblonga Bge.)是茜草科野丁香属的一种落叶灌木,产于我国华北、西南地区,常生长于山坡、路边等向阳处,也见于灌木丛中。据研究记载,薄皮木因花型似丁香花,又叫野丁香,因株型矮小,夏秋开花,在草坪、园林等作为观赏性植物种植,具有观赏价值。薄皮木的干枯、鲜嫩枝叶常被作为中低等饲用植物被牛羊等畜牧采食。因其植物化学成分含有较高粗脂肪含量,为牲蓄提供大量能量和营养,所以其具有饲用价值。目前,薄皮木的研究主要集中在种子萌发特性、叶表皮形态特征、快速繁殖技术与组织培养等内容,对该植物化学成分与药理作用的研究报道很少。鉴于以上发现,前期课题组对其地上部分的正丁醇部位和乙酸乙酯部位的化学成分做了初步研究。研究发现,正丁醇部位分离出6个化合物,乙酸乙酯部位分离出11个化合物,分别是降倍半萜苷类、三萜类、黄酮类、甾体类、木脂素类和其他类;为完善薄皮木的研究,我们主要对薄皮木乙酸乙酯部位的化学成分及药理活性进行了进一步的研究。目的意义:本文对薄皮木乙酸乙酯部位的化学成分进行研究,以期明确该植物中的化学成分,分离、鉴定单体化合物,对结构新颖可能存在药理活性的化合物进行活性研究,为后期薄皮木植物资源开发应用提供理论依据。方法:本文采用正相硅胶柱、反相ODS柱、反相MCI柱和Sephadex LH-20凝胶柱等柱填料进行化学成分分离纯化,并应用理化常数测定和现代波谱技术对所分离得到的化合物进行结构鉴定;利用NO一步法试剂盒对薄皮木不同极性大小的粗提物及分离得到的单体化合物进行抗炎活性研究。结论:(1)从薄皮木乙酸乙酯部位中分离鉴定出了15个化合物,13个化合物为首次从薄皮木中分离鉴定出来,其中黄酮类5个,三萜类6个,甾体类2个,其他类2个。分别是3,4',5,7-tetrahydroxy flavone(1)、3-oxo-oleanolic acid(2)、kaempferol-3-O-β-D-glucopyranoside(3)、isorhamnetin-3-O-β-Dglucopyranoside(4)、3,5,7-trihydroxy-4'-methoxy flavone(5)、betulinic acid(6)、quercetin(7)、1-octacosanol(8)、β-daucosterol(9)、ursolic aldehyde(10)、trilinolein(11)、β-sitosterol(12)、friedelane-2α,3β-diols(13)、3β-acetoxy-urs-11-en-28,13β-olide(14)、3β-hydroxyurs-12-ene(15)。(2)薄皮木总浸膏及不同极性部位浸膏对LPS刺激的RAW264.7小鼠巨噬细胞有不同程度的抑制作用,结果显示,相比于模型组,Et OAC Fr.和Bu OH Fr.作用的NO释放量有所减少(P0.05),其余不同组分作用的NO释放量随浓度的增加而减少,但抑制作用较弱,无显著性差异(P0.05);相比不同部位抑制率发现,抑制作用较强的为乙酸乙酯部位和正丁醇部位,浓度为200μg/m L时,抑制率分别为56.06%、57.03%,因此,乙酸乙酯部位和正丁醇部位可能含有具有抗炎作用的活性物质。除了化合物1-octacosanol(8)和trilinolein(11)之外,其余的单体化合物对LPS诱导的RAW264.7细胞释放的NO均有一定的抑制作用,但抑制作用较弱,与模型组对比,kaempferol-3-O-β-D-glucopyranoside(3),3,5,7-trihydroxy-4'-methoxyflavone(5),betulinic acid(6),β-daucosterol(9),ursolic aldehyde(10),β-sitosterol(12),3β-hydroxyurs-12-ene(15)在浓度为25μg/m L时,有显著性差异(P0.05),其余化合物无显著性差异(P0.05),且具有剂量依赖性。
[Abstract]:Background: Leptodermis Wall. (Leptodermis Wall.) is a genus of alizardae (Rubiaceae), about 40 species, distributed in the Himalaya region to Japan. According to the records of Chinese flora, there are 35 species, 9 varieties, 1 variants, and the north and south of China, but the main origin is the North China and the southwest part of the province. And Bopimu (Leptodermis oblonga Bge.) is alizari. A deciduous shrub of the genus lilaca of the genus lilaca, native to North China and southwest of China, often growing on hillsides, along the side of the side of the road, and also in the shrubs. According to research, the thin pellicus is like lilacs and is called No Choka. The plant type is small and flowering in summer and autumn. It is planted in lawn and gardens as ornamental plants, and has ornamental value. Thin skin wood. The fresh shoot leaves are often used as medium and low feed plants to be fed by cattle and sheep. Because the chemical composition of the plant contains high crude fat content, it provides a large amount of energy and nutrition for the animals, so it has a feeding value. At present, the research of thin skin is mainly concentrated on the seed germination characteristics, the leaf surface morphology, rapid propagation technology and the rapid propagation technology. There are few reports on the chemical composition and pharmacological action of the plant. In view of the above discovery, preliminary research group made a preliminary study on the chemical composition of n-butanol part and ethyl acetate part of the upper part of the plant. It was found that 6 compounds were separated from n-butanol and 11 compounds were separated from ethyl acetate. Compounds are terpenoid glycosides, three terpenoids, flavonoids, flavonoids, steroids, lignans and other classes. In order to improve the research of thin skinned wood, we mainly study the chemical composition and pharmacological activity of the ethyl acetate part of thin skin wood. The purpose is to study the chemical composition of the ethyl acetate part of the thin skin wood in this paper. The chemical components in the plant are identified, separated and identified, and the activity of the compounds with novel structure may exist in the study. The theoretical basis for the development and application of late thin skin wood plant resources is provided. Methods: This article uses the column of the normal silica gel, the reverse phase ODS column, the reverse phase MCI column and the Sephadex LH-20 gel column. The chemical constituents were isolated and purified, and the compounds were identified by physical and chemical constants and modern spectroscopy. The NO one step kit was used to study the anti-inflammatory activity of the crude extracts of different polar sizes and the isolated monomers. (1) separation from the ethyl acetate part of pellicle. 15 compounds were identified and 13 compounds were isolated and identified for the first time from thin skin wood, including 5 flavonoids, 6 three terpenoids, 2 steroids, 2 other classes, 3,4', 5,7-tetrahydroxy flavone (1), 3-Oxo-oleanolic acid (2), kaempferol-3-O- beta -D-glucopyranoside (3), isorhamnetin-3-O- beta -Dglucopyranoside (4), 3,5,7-tr. Ihydroxy-4'-methoxy flavone (5), betulinic acid (6), quercetin (7), 1-Octacosanol (8), beta -daucosterol (9), ursolic aldehyde (10), trilinolein (11), beta -sitosterol (12), friedelane-2 alpha, 3 beta, 13, 3 beta (14), 3 beta (15)). PS stimulated RAW264.7 mouse macrophages had varying degrees of inhibition. The results showed that the amount of NO release of Et OAC Fr. and Bu OH Fr. decreased (P0.05), while the NO release of the other components decreased with the increase of concentration, but the inhibitory production was weak and no significant difference (P0.05) was compared with the different parts. The rate of inhibition was found to be stronger in ethyl acetate and n-butanol. When the concentration was 200 g/m L, the inhibition rate was 56.06%, 57.03%. Therefore, ethyl acetate and n-butanol may contain anti-inflammatory substances. Besides compounds 1-Octacosanol (8) and trilinolein (11), the rest of the monomers The inhibition effect on NO released by LPS induced RAW264.7 cells was certain, but the inhibitory effect was weak. Compared with the model group, kaempferol-3-O- beta -D-glucopyranoside (3), 3,5,7-trihydroxy-4'-methoxyflavone (5), betulinic acid (6), beta -daucosterol (9), ursolic aldehyde (10), beta -sitosterol (12), 3 beta (15) were in the concentration of 2. There was a significant difference (P0.05) between 5 g/m L and the other compounds (P0.05), and it was dose-dependent.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1

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