沙棘三宝胶囊干预大鼠肝纤维化的作用

发布时间：2018-06-28 11:34

本文选题：沙棘三宝胶囊 + 肝纤维化　；参考：《青海大学》2017年硕士论文

【摘要】：目的:研究沙棘三宝胶囊对大鼠肝纤维化的影响。方法:将大鼠随机分为空白对照组(16只,生理盐水1.0 mL/kg)、阴性对照组(16只,橄榄油1.0 mL/kg)和肝纤维化模型组(40只,CCl_4+橄榄油1.0 mL/kg)。将成功造模大鼠分为模型组(8只,生理盐水1.0 mL/kg)、沙棘三宝胶囊组(每组8只,沙棘三宝胶囊低、中、高剂量组,剂量分别为0.13、0.26、0.65g/kg)、空白组(8只,生理盐水1.0 mL/kg)、阴性对照组(8只,橄榄油1.0 mL/kg)。每天灌胃给药1次,连续60天。使用生化分析仪检测血清中肝功能相关指标ALT、AST,使用放射免疫法检测透明质酸(HA)、Ⅳ型胶原(CIV)的变化并做组织病理学观察。结果:中、高剂量的沙棘三宝胶囊能够明显降低肝纤维化大鼠HA、ALT、AST含量(P0.05)并能抑制大鼠肝组织纤维化增生。其中,中剂量药物干预肝纤维化的作用更优。结论:沙棘三宝胶囊对大鼠肝纤维化有一定的干预作用。
[Abstract]:Objective: to study the effect of Hippophae rhamnoides Sanbao capsule on hepatic fibrosis in rats. Methods: the rats were randomly divided into control group (n = 16, physiological saline 1.0 mL / kg), negative control group (n = 16, olive oil 1.0 mL / kg) and hepatic fibrosis model group (n = 40, CCl4 olive oil 1.0 mL / kg). The successful model rats were divided into three groups: model group (8 rats, physiological saline 1.0 mL / kg), seabuckthorn Sanbao capsule group (8 rats in each group, low, medium and high dose groups, 0.130.26 ~ 0.65 g / kg), blank group (8, physiological saline 1.0 mL / kg), negative control group (8), Olive oil 1.0 mL / kg. Once a day by gavage for 60 days. Serum alt AST, HA and CIV were detected by biochemical analyzer and histopathological observation. Results: high dose of Hippophae rhamnoides Sanbao capsule could significantly reduce the content of HAA ALT AST in rats with hepatic fibrosis (P0.05) and inhibit the proliferation of hepatic fibrosis in rats. Among them, the effect of middle dose drugs on hepatic fibrosis is better. Conclusion: Hippophae rhamnoides Sanbao capsule has a certain effect on hepatic fibrosis in rats.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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