中空生物玻璃骨修复复合材料的制备与性能研究

发布时间：2018-07-05 17:13

  本文选题：中空生物玻璃 + 丝素蛋白　； 参考：《浙江理工大学》2017年硕士论文

【摘要】：近年来,由于人口老龄化、交通事故频发以及各种疑难疾病患者增多,造成骨组织和器官缺损的现象也越来越多。研制理想的用于骨组织修复的生物替代材料已经成为骨组织工程的热点;而人体骨组织修复生物医用纺织品亦成为现代生物医用纺织品的重要研究方向之一。丝素蛋白是天然蚕丝脱胶后的高分子纤维蛋白,因其独特的氨基酸,而具备良好的生物相容性和生物降解性,使得丝素蛋白在生物医学领域尤其是骨修复材料方面越来越成为研究者关注的焦点。然而,丝素蛋白在人体中,与骨结合能力较差、生物活性不高,这些缺点限制了其在骨修复材料的应用和发展。因此,本文通过丝素蛋白和中空生物玻璃球进行有机/无机的复合,并通过加入聚环氧乙烷来提高丝素蛋白的弹性,制备新型的SF/PEO/HBG复合纤维膜,系统研究中空生物玻璃球和SF/PEO/HBG复合纤维膜的制备、理化性能以及生物活性。具体研究内容如下:(1)中空生物玻璃球的制备及性能研究采用改进的溶胶-凝胶法,以聚丙烯酸(PAA)为软模板来制备PAA@BG核-壳生物玻璃球,通过煅烧去除PAA后,得到中空生物玻璃球(HBG);并通过FE-SEM、TEM、FT-IR、EDS、TG等测试方法对制备的中空生物玻璃球的结构、形貌及性能等进行表征。通过考察制备时间对中空生物玻璃球的影响,得出72h为最佳反应时间,钙盐、磷源能够全部稳定的包覆在中空SiO2表面,制备出形态及理化性能较好的中空生物玻璃球;热力学分析表明,煅烧温度为550℃,样品中的有机成分PAA和未反应完的钙和磷已经全部分解。(2)不同粒径的中空生物玻璃球的调控及生物活性研究通过改变TEOS/PAA的质量比,来调控中空生物玻璃球的粒径,并通过FE-SEM、TEM、粒径分布、FT-IR、EDS、XRD等测试方法对不同粒径的中空生物玻璃球的结构、形貌及性能等进行研究;再通过体外生物活性测试,分析其诱导生成羟基磷灰石的能力以及演化,考察其生物活性。结果表明:中空生物玻璃球为形状规整的球形、粒径均一、单分散性良好,且具有明显的中空结构,并且具有较强的诱导沉积羟基磷灰石的能力,生物活性良好。(3)SF/PEO/HBG骨修复复合材料的构建及生物活性研究蚕丝经脱胶、溶解成丝素蛋白溶液,加入PEO,搅拌均匀后制得SF/PEO混合溶液,再加入HBG,通过静电纺丝制得SF/PEO/HBG复合纤维膜,并通过FE-SEM、FT-IR、EDS、XRD等测试方法对SF/PEO/HBG复合纤维膜的形貌及性能进行表征;通过体外生物活性测试,分析其诱导生成羟基磷灰石的能力,考察其生物活性。结果表明:SF/PEO/HBG复合纤维膜经乙醇处理后,纤维状态良好,表面平整光滑,但纤维粗细不均匀,纤维间网络结构良好,并且具有强的羟基磷灰石形成能力,生物活性较好。由以上实验结果可知,SF/PEO/HBG复合纤维膜具有较好的理化性能和生物活性,有望成为一种用于骨缺损临床治疗的骨组织修复材料。
[Abstract]:In recent years, due to the aging of population, frequent traffic accidents and the increasing number of patients with various difficult diseases, the phenomenon of bone tissue and organ defects has become more and more. The development of ideal biomaterials for bone tissue repair has become a hot spot in bone tissue engineering, and biomedical textiles for human bone tissue repair have become one of the important research directions of modern biomedical textiles. Silk fibroin is a high molecular fibrin after degumming of natural silk. Because of its unique amino acid, silk fibroin has good biocompatibility and biodegradability. Silk fibroin has become the focus of researchers in biomedical field, especially in bone repair materials. However, silk fibroin has poor ability to bind to bone and low biological activity in human body. These shortcomings limit the application and development of silk fibroin in bone repair materials. Therefore, in this paper, a novel SF/ PEO / HBG composite fiber membrane was prepared by organic / inorganic composite of silk fibroin and hollow biological glass ball, and by adding polyethylene oxide to improve the elasticity of silk fibroin. The preparation, physical and chemical properties and biological activity of hollow bioglass ball and SFR / PEO / HBG composite fiber membrane were systematically studied. The main contents are as follows: (1) the preparation and properties of hollow biological glass spheres were studied. The PAABG core-shell biological glass spheres were prepared by modified sol-gel method and polyacrylic acid (PAA) as soft template. The PAA was removed by calcination. The hollow biological glass spheres (HBG) were prepared and the structure, morphology and properties of the hollow bioglass spheres were characterized by FE-SEMMA-TEMT-IRT-EDSTG and other methods. By investigating the effect of preparation time on hollow biological glass ball, the optimum reaction time is 72 h, calcium salt and phosphorus source can be coated on the surface of hollow Sio 2 stably, and the hollow biological glass ball with good morphology and physical and chemical properties can be prepared. The thermodynamic analysis shows that the organic components PAA and calcium and phosphorus in the sample have been completely decomposed at 550 鈩，

本文编号：2101044