七氟醚后处理对微管结构及心肌保护效果的影响

发布时间：2018-07-10 15:54

本文选题：七氟醚后处理 + 微管　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:探讨七氟醚后处理对小鼠心肌缺氧/复氧损伤时微管结构变化的影响,以及心肌实施七氟醚后处理的心肌保护效果的可行性分析。方法:首先培养小鼠乳鼠原代心肌细胞,建立缺氧/复氧损伤模型,将贴壁的心肌细胞随机分为5组:假手术组(sham组)、缺氧/复氧损伤(H/R组)、七氟醚后处理组(SPostC组)、七氟醚后处理组+紫杉醇组(SPostC+toxl组)、七氟醚后处理组+秋水仙素组(SPostC+Col组),免疫荧光染色后用共聚焦显微镜观察细胞微管分布、形态变化以及比较微管蛋白荧光强度。然后将C57小鼠(20-30g)40只,制备在体心肌缺血再灌注损伤(I/R)模型,随机分组同上;分别于缺血前、再灌组30min、120min后观察收缩压、心率等血流动力学指标,于再灌组120min比较各组左心室心梗面积;然后用蛋白质印迹法检测(western blot)心肌α微管蛋白的表达。结果:与缺氧/复氧损伤组(H/R)比较,七氟醚后处理组微管结构的损伤有所减轻,且微管α蛋白免疫荧光蛋白强度增加(P0.05);且能明显减少小鼠左心室心肌梗死面积(P0.05);在再灌组120min时,七氟醚后处理组血压升高(P0.05)、心率减慢(P0.05);western blot也证实与缺血再灌注组比较,七氟醚后处理组能明显增加α-微管蛋白的表达(P0.05)。结论:七氟醚后处理可以明显减轻缺血再灌注所致的微管结构的损伤,且具有一定的心肌保护作用,为微管相关的心肌保护提供一定的理论依据。
[Abstract]:Objective: to investigate the effect of sevoflurane post-treatment on the changes of microtubule structure during hypoxia / reoxygenation injury in mice and the feasibility analysis of myocardial protective effect of sevoflurane post-treatment. Methods: the primary cardiomyocytes of neonatal mice were cultured at first, and the model of hypoxia / reoxygenation injury was established. The adherent cardiomyocytes were randomly divided into 5 groups: sham operation group (sham group), hypoxia / reoxygenation injury group (H / R group), sevoflurane post-treatment group (SPostC group), sevoflurane post-treated paclitaxel group (SPostC toxl group) and sevoflurane post-treated colchicine group. The distribution of microtubules in SPostC Col group was observed by confocal microscope after immunofluorescence staining. Morphological changes and comparison of fluorescent intensity of tubulin. Then, 40 C57 mice (20-30g) were used to establish myocardial ischemia-reperfusion injury (I / R) model in vivo, and were randomly divided into the same group, systolic blood pressure, heart rate and other hemodynamic indexes were observed before ischemia and 30 min after reperfusion group. The area of left ventricular myocardial infarction (LVMI) was compared with that of reperfusion group (120min), and the expression of 伪 tubulin in (western blot) myocardium was detected by Western blotting. Results: compared with the hypoxia / reoxygenation group (H / R), the damage of microtubule structure in sevoflurane post-treated group was alleviated, and the intensity of microtubule 伪 protein immunofluorescence protein was increased (P0.05), and the myocardial infarction area of left ventricle in mice was significantly decreased (P0.05). In sevoflurane post-treatment group, the blood pressure was increased (P0.05), and the heart rate was decreased (P0.05). Western blot also confirmed that sevoflurane post-treated group could significantly increase the expression of 伪 -tubulin compared with the ischemia-reperfusion group (P0.05). Conclusion: sevoflurane post-treatment can obviously reduce the injury of microtubule structure induced by ischemia reperfusion and has certain myocardial protective effect, which provides a theoretical basis for microtubule-related myocardial protection.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

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