四十分钟深低温停循环对大鼠脑神经功能的影响的实验研究

发布时间：2018-10-05 11:06

【摘要】：目的:建立和改进大鼠深低温停循环模型,初步评估四十分钟DHCA对大鼠脑神经功能的影响。方法:随机选取20只SD大鼠,随机分为实验组(n=10)和对照组(n=10),两组大鼠均采用闭胸体外插管,实验组开始体外循环后建立DHCA模型(对照组只插管不行体外循环),术后均采用Morris水迷宫和旷场实验研究大鼠脑神经功能。结果:成功建立及改进了四十分钟DHCA大鼠模型,存活率达90%;经过研究发现:实验组大鼠Morris水迷宫的空间学习潜伏期更长,而且在目标象限内所呆的时间更短;旷场实验时在中央区域呆的时间更多,但移动距离与对照组相差不大。结论:四十分钟DHCA对大鼠脑神经功能有损伤;四十分钟DHCA大鼠模型是一个研究临床DHCA脑损伤及脑保护可靠的实验平台。
[Abstract]:Aim: to establish and improve the deep hypothermia circulatory arrest model in rats and to evaluate the effect of DHCA on the brain function of rats. Methods: twenty SD rats were randomly divided into experimental group (n = 10) and control group (n = 10). The experimental group established DHCA model after cardiopulmonary bypass (the control group was only intubated without cardiopulmonary bypass). The Morris water maze and open field experiment were used to study the brain nerve function of the rats. Results: the DHCA rat model was successfully established and improved for 40 minutes, and the survival rate was 90. The results showed that the Morris water labyrinth in the experimental group had a longer spatial learning latency and a shorter time in the target quadrant. In the open field experiment, the time spent in the central area was more, but the distance of movement was not different from that of the control group. Conclusion: forty minute DHCA can injure the brain function of rats, and 40 minutes DHCA rat model is a reliable experimental platform for the study of clinical DHCA brain injury and brain protection.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R654

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