外周血树突状细胞成熟度在泡型肝包虫患者中的研究

发布时间：2018-12-07 06:53

【摘要】：目的通过研究AE患者外周血中DC的成熟度,探究DC在AE免疫发病机制中的作用并分析其及与AE的发生发展的关系。方法1选取2015年9月至2016年6月于青海大学附属医院肝胆胰二科初诊并经病理证实为AE患者及随机抽取同期年龄相仿健康体检者各20例,采集外周空腹抗凝全血2ml,流式细胞仪检测外周血CD80~+细胞、CD86+细胞、CD83~+细胞及(CD80~++CD86++CD80~+)/CD14+比值。2选取2016年6月到2017年2月于青海大学附属医院肝胆胰二科初诊并经病理证实为AE患者及随机抽取同期年龄相仿健康体检者30例,加入细胞因子GM-CSF,IL-4及TNF-α进行体外培养诱导,获取大量DC,利用流式细胞仪检测DC表面CD83、CD86、CD80表达率。结果1 AE组与对照组外周血检测DC表面的共刺激分子(CD80~+CD83~+CD86)/CD14无统计学差异(P0.05),CD86细胞表达较对照组低(P0.05),且表达有统计学意义。2 AE组外周血单核源性培养DC表面的共刺激分子CD80、CD83、CD86的表达水平(41.71±8.16、39.69±8.05、50.79±7.72)均低于对照组CD80、CD83、CD86的表达水平(62.27±5.94、56.37±7.33、72.00±7.36),差异均有统计学意义(P0.05)。结论本研究显示AE患者外周血DC成熟度比对照组低,存在成熟障碍,可能会导致宿主抗原提呈功能下降,机体抗感染能力降低,从而促进了多房棘球蚴感染的进展。
[Abstract]:Objective to study the maturity of DC in peripheral blood of patients with AE, to explore the role of DC in the pathogenesis of AE and to analyze the relationship between DC and the occurrence and development of AE. Methods from September 2015 to June 2016, 20 patients with AE and 20 healthy people of the same age were selected from the second Department of Hepatopancreatology, affiliated Hospital of Qinghai University, from September 2015 to June 2016. The peripheral fasting anticoagulant whole blood (2ml) was collected. CD80~ cells and CD86 cells in peripheral blood were detected by flow cytometry. CD83~ cells and (CD80~ CD86 CD80~) / CD14 ratio were selected from June 2016 to February 2017 in the second Department of liver, Bile and pancreas, affiliated Hospital of Qinghai University and pathologically proved to be AE patients, and 30 healthy persons of similar age were randomly selected for the same period. A large number of DC, was obtained by the addition of cytokines GM-CSF,IL-4 and TNF- 伪 in vitro. The expression rate of CD83,CD86,CD80 on DC was detected by flow cytometry. Results 1 there was no significant difference in CD80~ CD83~ CD86 / CD14 on DC surface between AE group and control group (P0.05), but the expression of CD86 cells was lower in AE group than in control group (P0.05). 2 the expression of CD80,CD83,CD86 on the surface of mononuclear culture DC in AE group was significantly lower than that in control group (41.71 卤8.1659.69 卤8.05 卤50.79 卤7.72). The expression level of CD86 (62.27 卤5.94) was 56.37 卤7.33 卤72.00 卤7.36, the difference was statistically significant (P0.05). Conclusion this study shows that the maturity of peripheral blood DC in patients with AE is lower than that in the control group, which may lead to the decline of host antigen presentation function and the decrease of body anti-infection ability, thus promoting the progress of infection of echinococcus multilocularis.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R532.32

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