KAI1基因敲低表达对肺腺癌细胞增殖、侵袭及迁移的影响
发布时间:2021-12-31 13:32
目的:探讨KAI1基因敲低表达后对肺腺癌细胞生物学行为和功能的影响以及和肿瘤信号相关通路分子之间的关系。方法:构建目的基因KAI1基因敲低表达组和阴性对照组干扰片段siRNA,利用瞬时转染技术通过转染试剂Lpofectamin2000作用于两种肺腺癌细胞株A549和H1299,利用RT-PCR技术和Western blot技术在基因和蛋白水平分别检测目的基因在KAI1空白组、阴性对照组、敲低表达组的表达;MTT试剂盒法以及Transwell小室侵袭和迁移技术检测KAI1基因敲低表达后肺腺癌细胞功能变化情况;利用Western blot检测KAI1基因敲低表达后Axin1、GSK3β、vimentin和Slug的蛋白表达水平在各组中的变化情况;将慢病毒介导的KAI1过表达基因转染到人肺腺癌细胞A549后注射到裸鼠体内观测其对裸鼠移植瘤生长的影响,最后收集每组数据计算移植瘤的平均体积,连续观察5周后脱颈处死裸鼠并剥离瘤组织,并称取瘤组织的重量、测量瘤体体积。结果:两株细胞KAI1敲低表达组中的KAI1基因mRNA表达水平均比空白组和阴性对照组低,数据差异具有统计学意义(p<0.05)...
【文章来源】:蚌埠医学院安徽省
【文章页数】:62 页
【学位级别】:硕士
【部分图文】:
荧光定量PCR检测两株细胞KAI1mRNA的表达Figure1:FluorescencequantitativePCRdetectionofKAI1mRNAexpressionintwocells
22图1:荧光定量PCR检测两株细胞KAI1mRNA的表达Figure1:FluorescencequantitativePCRdetectionofKAI1mRNAexpressionintwocells图2:两株细胞干扰组中KAI1mRNA的相对表达量明显低于空白组和NC组(p<0.05)Figure2:TherelativeexpressionofKAI1mRNAintwocellinterferencegroupswassignificantlylowerthanthatinblankgroupandNCgroup(p<0.05)
KAI1 KAI1 Tubulin Tubulin 图 3:两株细胞各实验组蛋白表达 Figure 3: Protein expression in each experimental group of two cells
【参考文献】:
期刊论文
[1]Expression and clinical significance of p53,JunB and KAI1/CD82 in human hepatocellular carcinoma[J]. Cheng Guo,Qing-Guang Liu,Lei Zhang,Tao Song and Xue YangDepartment of Hepatobiliary Surgery,First Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710061, China. Hepatobiliary & Pancreatic Diseases International. 2009(04)
[2]KAI1 gene expression in colonic carcinoma and its clinical significances[J]. De-Hua Wu Li Liu Long-Hua Chen Yan-Qing Ding Department of Radiation Oncology,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaDepartment of Pathology,First Military Medical University,Guangzhou 510515,Guangdong Province,China. World Journal of Gastroenterology. 2004(15)
本文编号:3560347
【文章来源】:蚌埠医学院安徽省
【文章页数】:62 页
【学位级别】:硕士
【部分图文】:
荧光定量PCR检测两株细胞KAI1mRNA的表达Figure1:FluorescencequantitativePCRdetectionofKAI1mRNAexpressionintwocells
22图1:荧光定量PCR检测两株细胞KAI1mRNA的表达Figure1:FluorescencequantitativePCRdetectionofKAI1mRNAexpressionintwocells图2:两株细胞干扰组中KAI1mRNA的相对表达量明显低于空白组和NC组(p<0.05)Figure2:TherelativeexpressionofKAI1mRNAintwocellinterferencegroupswassignificantlylowerthanthatinblankgroupandNCgroup(p<0.05)
KAI1 KAI1 Tubulin Tubulin 图 3:两株细胞各实验组蛋白表达 Figure 3: Protein expression in each experimental group of two cells
【参考文献】:
期刊论文
[1]Expression and clinical significance of p53,JunB and KAI1/CD82 in human hepatocellular carcinoma[J]. Cheng Guo,Qing-Guang Liu,Lei Zhang,Tao Song and Xue YangDepartment of Hepatobiliary Surgery,First Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710061, China. Hepatobiliary & Pancreatic Diseases International. 2009(04)
[2]KAI1 gene expression in colonic carcinoma and its clinical significances[J]. De-Hua Wu Li Liu Long-Hua Chen Yan-Qing Ding Department of Radiation Oncology,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaDepartment of Pathology,First Military Medical University,Guangzhou 510515,Guangdong Province,China. World Journal of Gastroenterology. 2004(15)
本文编号:3560347
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