脱酚棉籽蛋白对鸡蛋品质的影响及其机理
发布时间：2018-08-06 13:24
【摘要】：近年来,随着大豆对外依存度的增加,价格稳定性变差,营养学家试图通过各种营养调控手段,加大对杂粕资源的利用。作为一种新型粕类蛋白,脱酚棉籽蛋白(Low Gossypol Cottonseed Meal,LCSM)具有低棉酚和高蛋白的特点,质量好于传统棉粕,但其在蛋鸡日粮中适宜使用比例、对蛋鸡和鸡蛋的影响及其机理鲜有报道。本文采用饲养试验、蛋品质和血液生化测定、i TRAQ蛋白质组学、组织学、双向凝胶电泳结合基质辅助激光解析电离飞行时间质谱分析等方法,研究了LCSM对产蛋鸡生产性能、蛋品质、、器官组织学、血液生化和蛋清蛋白相对含量等的影响。开展本项目研究,可以厘清棉籽蛋白的特点,有利于充分利用我国丰富的棉籽资源,拓宽棉籽蛋白的应用领域。试验一不同日粮蛋白水平脱酚棉籽蛋白对蛋鸡生产性能及蛋品质的影响本试验观察了不同蛋白水平下,日粮LCSM对蛋鸡生产性能及其蛋品质的影响,确定添加LCSM时,适宜的饲粮粗蛋白水平。选择40 WK健康海兰褐产蛋鸡432只,随机分为6个处理,每个处理6个重复,每重复12羽,分别采食以等能、相同理想氨基酸模式配制的3个蛋白水平(CP 17.5%、16%和14.5%)的玉米-豆粕(SBM)或玉米-脱酚棉籽蛋白日粮,预饲期1周,正饲期12周。试验期间,每天记录蛋重、耗料量,计算料蛋比等生产性能指标。每隔6周,每重复收集5枚鸡蛋,测定蛋品质(蛋白高度、哈氏单位(HU)、蛋壳强度、厚度、蛋黄重和蛋黄颜色)。结果表明,试验全期,LCSM未显著影响蛋壳厚度、强度、蛋黄颜色和蛋黄重;提示,LCSM不影响蛋壳和蛋黄品质。与SBM组相比,在40~45 WK没有显著影响,但46~51 WK,不同蛋白水平下,LCSM对蛋鸡采食量、蛋重和蛋清品质影响显著(P0.05)。日粮CP 14.5%,全棉籽蛋白和全豆粕日粮,未见显著影响蛋鸡生产性能;日粮CP 16%和17.5%时,与全豆粕组相比,在46~51 WK,LCSM组的蛋鸡采食量和蛋重均显著降低(P0.05)。日粮CP 16%,LCSM组鸡蛋蛋白高度显著降低(P0.05)。全豆粕型日粮,与CP 17.5%组相比,CP 14.5%组的蛋重和蛋白高度显著降低(P0.05)。全棉籽蛋白组和全豆粕组中,与CP 17.5%组相比,CP 16%和14.5%组的饲料/鸡蛋最低,但CP 14.5%组中蛋白高度和HU显著降低。因此,在使用脱酚棉籽蛋白时,日粮蛋白水平适宜采用16%;LCSM日粮饲喂蛋鸡6周未见显著影响生产性能,12周引起了生产性能和蛋清品质的下降,提示LCSM对产蛋鸡生产性能有累积致毒作用。试验二脱酚棉籽蛋白对蛋鸡生产性能及蛋品质的影响本试验研究了日粮中添加的LCSM,对蛋鸡生产性能及蛋品质的影响,以确定LCSM的适宜添加水平。选择432只40 WK健康海兰褐产蛋鸡,随机分为6个处理,每个处理6个重复,每重复12羽鸡,分别采食等能、相同理想氨基酸模式的6种日粮(CP 16%)。对照组为玉米豆粕基础日粮,4个组(LCSM25、LCSM50、LCSM75和LCSM_100)分别按25%、50%、75%和_100%替代对照组中由豆粕提供的蛋白(添加50、98.3、144.2和189 g/kg LCSM),第6组在对照组日粮中添加和LCSM_100组含量相同的游离棉酚(28.35 mg/kg)。预饲期1周,正饲期12周。试验期间,每天记录蛋重、耗料量,计算料蛋比等生产性能指标。每隔6周,每个重复收集5枚鸡蛋,测定蛋品质及蛋清蛋白含量。研究表明,LCSM_100组46~51 WK蛋鸡采食量和蛋重显著降低(P0.05),全期,LCSM对蛋鸡产蛋率和料蛋比没有显著影响(P0.05)。试验全期,各处理组蛋黄颜色没有显著变化,但与豆粕组相比,LCSM_100组46~51 WK鸡蛋HU、蛋白高度和蛋清蛋白含量显著降低(P0.05)。与对照组相比,LCSM组的血浆总蛋白、尿素氮、尿酸、血清蛋白、丙氨酸转氨酶和天冬氨酸转氨酶没有显著变化;肝肾切片组织学未见病变。LCSM_100显著降低了蛋鸡采食量、蛋重及蛋清指标;然而,与豆粕组相比,添加棉酚组中各指标未见显著降低。因此,这种负面影响并非由其中棉酚引起;蛋鸡日粮中LCSM适宜添加98.3 g/kg。试验三脱酚棉籽蛋白对蛋清中蛋白质组成的影响本试验研究了LCSM引起的蛋清品质下降与蛋清中蛋白质相对含量之间的关系。将40 WK、216只海兰褐产蛋鸡分为3个组,对照组饲喂玉米-豆粕基础日粮(SBM组),另两组分别由LCSM替代对照组日粮中豆粕提供的50%(LCSM50)和_100%蛋白(LCSM_100)。试验期12周。试验期间,观测和记录蛋清、血液激素,观察输卵管组织学,采用i TRAQ技术研究饲喂LCSM后蛋清蛋白相对含量的差异。与SBM组相比,LCSM_100组蛋清中有15种蛋白相对含量显著降低,分别为:卵清蛋白(Ovalbumin)、卵转运铁蛋白(Ovotransferrin)、卵粘蛋白(Ovomucin)、溶菌酶蛋白(Lysozyme)、卵抑制剂(Ovoinhibitor)和簇蛋白(Clusterin)等。这些降低的蛋白占蛋清总蛋白的75%,均具有抗菌、抗病毒性,在蛋清的保护机制上发挥重要作用。卵粘蛋白影响蛋清粘度系数和抗蛋清稀化;簇蛋白影响蛋清中折叠蛋白的稳定和抗蛋白沉淀。饲喂LCSM后引起的这些蛋白的降低,造成了蛋白高度和HU的降低。LCSM_100组血浆孕酮水平的下降在一定程度抑制了输卵管中管状腺细胞和上皮细胞的生长,降低了该部位分泌蛋清蛋白的功能。分子水平上蛋清各蛋白的相对含量的下降阐释了其生化水平上各指标的下降,从分子水平说明了LCSM在蛋鸡日粮中不能_100%替代豆粕。试验四脱酚棉籽蛋白对储存期鸡蛋蛋品质及蛋清中蛋白质组成的影响本试验研究了LCSM对储存期蛋品质的影响及其机理。在饲养试验的12周末的连续三天内采集SBM、LCSM50和LCSM_100组蛋样总共540枚,分别储存于4℃,相对湿度65%下4和8周,测定0、4和8周的HU、蛋白p H、蛋黄指数;采用二维双向凝胶电泳(2-DE)结合基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF/TOF MS),分析储存8周后SBM组和LCSM_100组其蛋清蛋白的相对含量差异。结果显示,储存时间显著影响鸡蛋HU,随储存时间的延长HU显著降低(P0.05)。与SBM组相比,LCSM_100组HU低于SBM和LCSM50组(P0.05),鸡蛋蛋清p H显著高于SBM和LCSM50组的鸡蛋蛋清p H(P0.05)。储存期内,饲粮蛋白源显著影响鸡蛋蛋黄指数(P0.05),LCSM50组的蛋黄指数与SBM组无显著差异,但LCSM_100组低于LCSM50和SBM组(P0.05)。LCSM50组的蛋清品质与SBM组的蛋清品质相当。2-DE鉴定结果显示储存8周后SBM组由于储存时间的蛋清稀化作用有18个蛋白相对含量下降,而LCSM_100组中有36个蛋白相对含量上升,相比于上一章中i TRAQ鉴定的LCSM_100组中下降的15个蛋白数相比,其储存期下降蛋白增加了140%,说明LCSM_100组的鸡蛋在储存期其蛋品质更易趋于降低。LCSM_100组的鸡蛋由于其蛋清蛋白模式的改变在储存期不仅蛋清品质进一步降低,且蛋黄指数也显著下降,在整个试验期内,LCSM50组鸡蛋的蛋品质较LCSM_100更加稳定。
[Abstract]:In recent years, with the increase of the degree of dependence on soybean, the stability of the price has become worse and the nutritionist tries to use a variety of nutritional control methods to increase the use of mixed meal. As a new type of meal protein, the Low Gossypol Cottonseed Meal (LCSM) has the characteristics of low gossypol and high protein, and the quality is better than the traditional cottonseed, but the quality is better than that of the traditional cottonseed. In this paper, the effect of LCSM on laying hens and eggs and their mechanism were rarely reported. In this paper, the breeding experiments, egg quality and blood biochemical determination, I TRAQ proteomics, histology, bi-directional gel electrophoresis combined with matrix assisted laser analytical ionization time-of-flight mass spectrometry analysis were used to study the production of laying hens production by LCSM. Performance, egg quality, organ histology, blood biochemistry and the relative content of egg white protein. The study of this project can clarify the characteristics of cottonseed protein, make full use of the rich cottonseed resources in our country and broaden the application field of cottonseed protein. And the effect of egg quality on the production performance and egg quality of laying hens under different protein levels, the effects of dietary LCSM on egg quality and egg quality were determined. When adding LCSM, the appropriate dietary crude protein level was determined. 40 WK healthy sea blue brown laying hens were selected, and 432 eggs were randomly divided into 6 treatments, each treatment was repeated with 12 feathers, and the same energy was equal. Corn soybean meal (SBM) or corn - Dephenol cottonseed protein diet of 3 protein levels (CP 17.5%, 16% and 14.5%) prepared by the ideal amino acid pattern was pre fed for 1 weeks and 12 weeks in positive feeding period. During the test, the egg weight, material consumption, and the ratio of egg to egg were recorded every day. Every 6 weeks, 5 eggs were collected repeatedly and the egg quality (protein height was measured). Ha unit (HU), eggshell strength, thickness, yolk weight and yolk color. The results showed that LCSM did not significantly affect eggshell thickness, strength, yolk color and yolk weight, suggesting that LCSM did not affect the quality of eggshell and yolk. Compared with the SBM group, there was no significant effect on 40~45 WK, but 46~51 WK, at different protein levels, LCSM to laying feed on laying hens, Egg weight and egg white quality were significantly affected (P0.05). Dietary CP 14.5%, whole cotton seed protein and whole soybean meal had no significant effect on egg production performance. When dietary CP 16% and 17.5%, the feed intake and egg weight of egg chickens in group 46~51 WK and LCSM group decreased significantly (P0.05). Diet CP 16%, LCSM group egg protein significantly decreased (P0.05). Compared with the CP 17.5% group, the egg weight and protein height of the CP 14.5% groups decreased significantly (P0.05). In the whole cotton seed protein group and the whole soybean meal group, the diet / eggs of CP 16% and 14.5% groups were the lowest compared with the group CP 17.5%, but the protein height and HU in the CP 14.5% groups were significantly reduced. Therefore, the dietary protein level was used when the cottonseed protein was used. It is suitable to use 16%; LCSM diet feeding laying hens for 6 weeks has not significantly affected the production performance, the production performance and the quality of egg white have been reduced in 12 weeks, suggesting that LCSM has a cumulative toxic effect on the production performance of laying hens. Experiment two the effect of Dephenol cottonseed protein on the production performance and egg quality of laying hens in this experiment was to study the LCSM added in the diet and to the laying hens. The effects of production performance and egg quality were determined to determine the suitable addition level of LCSM. 432 40 WK healthy sea blue brown laying hens were selected and randomly divided into 6 treatments, each treated with 6 repetitions, each repeated 12 feathered chickens, 6 diets with the same ideal amino acid pattern (CP 16%). The control group was the basal diet of corn soybean meal, 4 groups (LCSM25, LCSM). 50, LCSM75 and LCSM_100) replaced the protein (adding 50,98.3144.2 and 189 g/kg LCSM) in the control group by 25%, 50%, 75% and _100% respectively. The sixth groups added the free gossypol (28.35 mg/kg) with the same content of the LCSM_100 group in the control group. The pre feeding period was 1 weeks and the positive feeding period was 12 weeks. During the test, the egg weight, material consumption, and calculation material were recorded daily. Egg ratio and other production performance indexes. Every 6 weeks, 5 eggs were collected repeatedly, and egg quality and egg white protein content were measured. The study showed that the feed intake and egg weight of 46~51 WK laying hens in LCSM_100 group decreased significantly (P0.05). LCSM had no significant influence on laying rate and egg ratio (P0.05) in the whole period (P0.05). The color of egg yolk in each treatment group was not significant during the whole period of the experiment. But compared with the soybean meal group, the LCSM_100 group 46~51 WK egg HU, the protein height and the egg white protein content decreased significantly (P0.05). Compared with the control group, the total plasma protein, urea nitrogen, uric acid, serum protein, alanine aminotransferase and aspartate aminotransferase in the LCSM group did not change significantly, and the liver and kidney section histology did not significantly reduce the pathological changes of.LCSM_100. The feed intake, egg weight and egg white index were lower, however, compared with the soybean meal group, the indexes in the addition of gossypol were not significantly reduced. Therefore, this negative effect was not caused by gossypol. The effect of LCSM on the protein composition of egg white in the diet of 98.3 g/kg. was suitable for the 98.3 g/kg. test. The relationship between the decrease of egg white quality and the relative content of protein in egg white. 40 WK, 216 blue brown laying hens were divided into 3 groups, the control group was fed with corn soybean meal base diet (SBM group), and the other two groups were replaced by 50% (LCSM50) and _100% protein (LCSM_100) from the diet of the control group, respectively. The test period was 12 weeks. During the test period, the observation period Test and record egg white, blood hormone, observe oviduct histology, use I TRAQ technique to study the difference of the relative content of egg white protein after feeding LCSM. Compared with group SBM, the relative content of 15 kinds of protein in group LCSM_100 was significantly reduced, respectively: ovalbumin (Ovalbumin), egg transporter ferritin (Ovotransferrin), ovalbumin (Ovomucin) and soluble protein, respectively. Bacteria enzyme protein (Lysozyme), egg inhibitor (Ovoinhibitor) and cluster protein (Clusterin). These reduced proteins account for 75% of the total egg white protein. They all have antibacterial, disease resistance, and play an important role in the protection mechanism of egg whites. Ooprotein affects the viscosity coefficient of egg white and the dilute of egg white. And anti protein precipitation. The decrease of these proteins caused by LCSM, resulting in the decrease of protein height and HU, decreased the growth of tubular and epithelial cells in the fallopian tubes to a certain extent, and reduced the function of the secreted egg white protein in this part of the oviduct. At molecular level, the relative proteins of egg white were relative to the molecular level. The decrease in content explained the decline of the indexes on the biochemical level. The effect of LCSM on the quality of egg and egg in storage period and the protein composition of egg white in the storage period was tested by the molecular level. The effect of LCSM on the quality of stored eggs and its mechanism were studied in the experiment. The effect of LCSM on the quality of stored eggs and its mechanism were studied. SBM, LCSM50 and LCSM_100 egg samples were collected at 12 weekends for three days, and 540 eggs were stored at 4, relative humidity 65% 4 and 8 weeks respectively. HU, protein P H, yolk index were measured for 0,4 and 8 weeks, and two dimensional two-dimensional gel electrophoresis (2-DE) combined with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used to analyze storage 8. The relative contents of egg white protein in group SBM and LCSM_100 group were significantly different. The results showed that the storage time significantly affected the egg HU, and the HU decreased significantly with the prolongation of storage time (P0.05). Compared with the SBM group, the HU was lower than the SBM and LCSM50 groups (P0.05), and the egg egg white P was significantly higher than that of the egg white group. The egg yolk index (P0.05) was significantly affected by the dietary protein source. The egg yolk index in group LCSM50 was not significantly different from that in SBM group, but the LCSM_100 group was lower than the LCSM50 and SBM group (P0.05).LCSM50 group and the quality of the egg white in the SBM group was equivalent to the.2-DE identification results showed that the SBM group had 18 proteins relative to the storage time after 8 weeks of storage. The content of 36 proteins in the LCSM_100 group increased. Compared to the 15 proteins in the LCSM_100 group identified in the previous chapter, the decrease in the storage period increased by 140%, indicating that the egg quality of the LCSM_100 group was more likely to tend to lower the egg quality of the.LCSM_100 group in the storage period of the LCSM_100 group because of its egg white protein pattern. In the storage period, the egg white quality was further reduced and the yolk index decreased significantly. In the whole period of the test, the egg quality of the LCSM50 group was more stable than that of LCSM_100.
【学位授予单位】：中国农业科学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S831.5
本文编号：2167864
[Abstract]:In recent years, with the increase of the degree of dependence on soybean, the stability of the price has become worse and the nutritionist tries to use a variety of nutritional control methods to increase the use of mixed meal. As a new type of meal protein, the Low Gossypol Cottonseed Meal (LCSM) has the characteristics of low gossypol and high protein, and the quality is better than the traditional cottonseed, but the quality is better than that of the traditional cottonseed. In this paper, the effect of LCSM on laying hens and eggs and their mechanism were rarely reported. In this paper, the breeding experiments, egg quality and blood biochemical determination, I TRAQ proteomics, histology, bi-directional gel electrophoresis combined with matrix assisted laser analytical ionization time-of-flight mass spectrometry analysis were used to study the production of laying hens production by LCSM. Performance, egg quality, organ histology, blood biochemistry and the relative content of egg white protein. The study of this project can clarify the characteristics of cottonseed protein, make full use of the rich cottonseed resources in our country and broaden the application field of cottonseed protein. And the effect of egg quality on the production performance and egg quality of laying hens under different protein levels, the effects of dietary LCSM on egg quality and egg quality were determined. When adding LCSM, the appropriate dietary crude protein level was determined. 40 WK healthy sea blue brown laying hens were selected, and 432 eggs were randomly divided into 6 treatments, each treatment was repeated with 12 feathers, and the same energy was equal. Corn soybean meal (SBM) or corn - Dephenol cottonseed protein diet of 3 protein levels (CP 17.5%, 16% and 14.5%) prepared by the ideal amino acid pattern was pre fed for 1 weeks and 12 weeks in positive feeding period. During the test, the egg weight, material consumption, and the ratio of egg to egg were recorded every day. Every 6 weeks, 5 eggs were collected repeatedly and the egg quality (protein height was measured). Ha unit (HU), eggshell strength, thickness, yolk weight and yolk color. The results showed that LCSM did not significantly affect eggshell thickness, strength, yolk color and yolk weight, suggesting that LCSM did not affect the quality of eggshell and yolk. Compared with the SBM group, there was no significant effect on 40~45 WK, but 46~51 WK, at different protein levels, LCSM to laying feed on laying hens, Egg weight and egg white quality were significantly affected (P0.05). Dietary CP 14.5%, whole cotton seed protein and whole soybean meal had no significant effect on egg production performance. When dietary CP 16% and 17.5%, the feed intake and egg weight of egg chickens in group 46~51 WK and LCSM group decreased significantly (P0.05). Diet CP 16%, LCSM group egg protein significantly decreased (P0.05). Compared with the CP 17.5% group, the egg weight and protein height of the CP 14.5% groups decreased significantly (P0.05). In the whole cotton seed protein group and the whole soybean meal group, the diet / eggs of CP 16% and 14.5% groups were the lowest compared with the group CP 17.5%, but the protein height and HU in the CP 14.5% groups were significantly reduced. Therefore, the dietary protein level was used when the cottonseed protein was used. It is suitable to use 16%; LCSM diet feeding laying hens for 6 weeks has not significantly affected the production performance, the production performance and the quality of egg white have been reduced in 12 weeks, suggesting that LCSM has a cumulative toxic effect on the production performance of laying hens. Experiment two the effect of Dephenol cottonseed protein on the production performance and egg quality of laying hens in this experiment was to study the LCSM added in the diet and to the laying hens. The effects of production performance and egg quality were determined to determine the suitable addition level of LCSM. 432 40 WK healthy sea blue brown laying hens were selected and randomly divided into 6 treatments, each treated with 6 repetitions, each repeated 12 feathered chickens, 6 diets with the same ideal amino acid pattern (CP 16%). The control group was the basal diet of corn soybean meal, 4 groups (LCSM25, LCSM). 50, LCSM75 and LCSM_100) replaced the protein (adding 50,98.3144.2 and 189 g/kg LCSM) in the control group by 25%, 50%, 75% and _100% respectively. The sixth groups added the free gossypol (28.35 mg/kg) with the same content of the LCSM_100 group in the control group. The pre feeding period was 1 weeks and the positive feeding period was 12 weeks. During the test, the egg weight, material consumption, and calculation material were recorded daily. Egg ratio and other production performance indexes. Every 6 weeks, 5 eggs were collected repeatedly, and egg quality and egg white protein content were measured. The study showed that the feed intake and egg weight of 46~51 WK laying hens in LCSM_100 group decreased significantly (P0.05). LCSM had no significant influence on laying rate and egg ratio (P0.05) in the whole period (P0.05). The color of egg yolk in each treatment group was not significant during the whole period of the experiment. But compared with the soybean meal group, the LCSM_100 group 46~51 WK egg HU, the protein height and the egg white protein content decreased significantly (P0.05). Compared with the control group, the total plasma protein, urea nitrogen, uric acid, serum protein, alanine aminotransferase and aspartate aminotransferase in the LCSM group did not change significantly, and the liver and kidney section histology did not significantly reduce the pathological changes of.LCSM_100. The feed intake, egg weight and egg white index were lower, however, compared with the soybean meal group, the indexes in the addition of gossypol were not significantly reduced. Therefore, this negative effect was not caused by gossypol. The effect of LCSM on the protein composition of egg white in the diet of 98.3 g/kg. was suitable for the 98.3 g/kg. test. The relationship between the decrease of egg white quality and the relative content of protein in egg white. 40 WK, 216 blue brown laying hens were divided into 3 groups, the control group was fed with corn soybean meal base diet (SBM group), and the other two groups were replaced by 50% (LCSM50) and _100% protein (LCSM_100) from the diet of the control group, respectively. The test period was 12 weeks. During the test period, the observation period Test and record egg white, blood hormone, observe oviduct histology, use I TRAQ technique to study the difference of the relative content of egg white protein after feeding LCSM. Compared with group SBM, the relative content of 15 kinds of protein in group LCSM_100 was significantly reduced, respectively: ovalbumin (Ovalbumin), egg transporter ferritin (Ovotransferrin), ovalbumin (Ovomucin) and soluble protein, respectively. Bacteria enzyme protein (Lysozyme), egg inhibitor (Ovoinhibitor) and cluster protein (Clusterin). These reduced proteins account for 75% of the total egg white protein. They all have antibacterial, disease resistance, and play an important role in the protection mechanism of egg whites. Ooprotein affects the viscosity coefficient of egg white and the dilute of egg white. And anti protein precipitation. The decrease of these proteins caused by LCSM, resulting in the decrease of protein height and HU, decreased the growth of tubular and epithelial cells in the fallopian tubes to a certain extent, and reduced the function of the secreted egg white protein in this part of the oviduct. At molecular level, the relative proteins of egg white were relative to the molecular level. The decrease in content explained the decline of the indexes on the biochemical level. The effect of LCSM on the quality of egg and egg in storage period and the protein composition of egg white in the storage period was tested by the molecular level. The effect of LCSM on the quality of stored eggs and its mechanism were studied in the experiment. The effect of LCSM on the quality of stored eggs and its mechanism were studied. SBM, LCSM50 and LCSM_100 egg samples were collected at 12 weekends for three days, and 540 eggs were stored at 4, relative humidity 65% 4 and 8 weeks respectively. HU, protein P H, yolk index were measured for 0,4 and 8 weeks, and two dimensional two-dimensional gel electrophoresis (2-DE) combined with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used to analyze storage 8. The relative contents of egg white protein in group SBM and LCSM_100 group were significantly different. The results showed that the storage time significantly affected the egg HU, and the HU decreased significantly with the prolongation of storage time (P0.05). Compared with the SBM group, the HU was lower than the SBM and LCSM50 groups (P0.05), and the egg egg white P was significantly higher than that of the egg white group. The egg yolk index (P0.05) was significantly affected by the dietary protein source. The egg yolk index in group LCSM50 was not significantly different from that in SBM group, but the LCSM_100 group was lower than the LCSM50 and SBM group (P0.05).LCSM50 group and the quality of the egg white in the SBM group was equivalent to the.2-DE identification results showed that the SBM group had 18 proteins relative to the storage time after 8 weeks of storage. The content of 36 proteins in the LCSM_100 group increased. Compared to the 15 proteins in the LCSM_100 group identified in the previous chapter, the decrease in the storage period increased by 140%, indicating that the egg quality of the LCSM_100 group was more likely to tend to lower the egg quality of the.LCSM_100 group in the storage period of the LCSM_100 group because of its egg white protein pattern. In the storage period, the egg white quality was further reduced and the yolk index decreased significantly. In the whole period of the test, the egg quality of the LCSM50 group was more stable than that of LCSM_100.
【学位授予单位】：中国农业科学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S831.5
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