玉米抗禾谷镰刀菌的转录组分析

发布时间：2018-12-13 18:41

【摘要】：赤霉菌茎腐病是由禾谷镰刀菌(Fusarium graminearum,有性态,Gibberella zeae)引起的一类土传性病害,严重危害玉米的产量和品质。本研究依据玉米第10和第1染色体上的2个抗茎腐病QTL,q Rfg1和q Rfg2、培育近等基因系NIL-R(2个QTL位点均为抗病等位基因)和NIL-S(2个QTL位点均为感病等位基因)。在成株期和幼苗期接种禾谷镰刀菌,两近等基因系的抗性差异均显著。用2个近等基因系的幼根接种禾谷镰刀菌,进行转录组分析研究。结果表明,与NIL-S相比,NIL-R在接种禾谷镰刀菌后,乙烯(ethylene,ET)合成、信号途径基因,病程相关蛋白、脱氧雪腐镰刀菌烯醇毒素(deoxynivalenol,DON)解毒基因等呈现特异上调表达。与NIL-S相比,有1170个基因在NIL-R对照组中表达量较高,其中水杨酸(salicylic acid,SA)、茉莉酸(jasmonic acid,JA)和乙烯合成和信号介导途径以及苯丙烷合成途径中的基因显著富集;接种禾谷镰刀菌6 h或18 h后,病程相关蛋白、激素JA和ET合成基因、DON解毒基因在NIL-R中表达量较高。
[Abstract]:Gibberellosis is a kind of soil-borne disease caused by (Fusarium graminearum, sexual, Gibberella zeae) of Fusarium graminearum, which seriously harms the yield and quality of maize. In this study, the near isogenic lines NIL-R (two QTL loci are disease-resistant alleles) and NIL-S (two QTL loci are susceptible alleles) were cultured on maize chromosomes 10 and 1 by QTL,q Rfg1 and Q Rfg2,. When inoculated with Fusarium graminearum at the adult stage and seedling stage, the resistance of the two isogenic lines was significantly different. Two near isogenic lines of young roots were inoculated with Fusarium graminearum for transcriptome analysis. The results showed that compared with NIL-S, NIL-R inoculated with Fusarium graminearum resulted in ethylene (ethylene,ET) synthesis, signal pathway gene, pathogenesis-related protein, and deoxynivalenol, (deoxynivalenol toxin). DON) detoxification genes and other genes showed a specific up-regulation. Compared with NIL-S, 1170 genes were highly expressed in NIL-R control group, including salicylic acid (salicylic acid,SA), jasmonic acid (jasmonic acid,JA), ethylene biosynthesis and signaling pathway, and phenylpropane synthesis pathway. After inoculation with Fusarium graminearum for 6 h or 18 h, the synthesis genes of pathogenesis-related protein, hormone JA and ET, and DON detoxification gene were highly expressed in NIL-R.
【作者单位】：中国农业大学国家玉米改良中心;
【基金】：引进国际先进农业科学技术计划(948计划)项目(2003-Q04)资助~~
【分类号】：S435.131.4

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