水杨酸和谷胱甘肽调控铬胁迫下油菜不同耐性品种生理生化和基因组变化的作用机理

发布时间：2021-01-18 12:49

　　土壤中重金属的毒害是制约油菜产量的主要因素之一。筛选对重金属抗性高的基因型是最佳解决方法之一,使用不同的植物生长调节剂提高非生物胁迫下长势也有显著效果。本研究包括四部分试验内容,取得结果如下：（1）试验以四个不同甘蓝型油菜品种（浙双758、浙大619、浙油50、浙大622）的6天幼苗为试验材料,研究铬（cr）的毒害作用。结果表明,随着铬浓度的升高,所有品种的幼苗生长均受到抑制,光合色素含量和抗氧化酶活性显著降低,丙二醛（MDA）和活性氧（ROS）的含量上升。相对于其他品种,浙大622的各个部位的铬含量均较高。电镜结果表明,400μM铬处理下,叶肉和根尖细胞超微结构被完全破坏,浙大622的破坏效果最显著。因此,浙大622相对于其他3个油菜品种表现更敏感。（2）选取了水培生长下的四个不同甘蓝型油菜品种来研究铬的毒性。结果表明,随着铬浓度的升高,油菜的生物量有所降低。而浙大622下降幅度最为显著,其光合作用降低,而浙双758在铬的毒害下相对表现较好。不同浓度的铬诱导了活性氧和丙二醛的积累。然而,为了清除活性氧和丙二醛的积累,相关的酶活性在叶片和根中均升高。电镜结果表明,400μM铬处理下浙大... 

【文章来源】：浙江大学浙江省 211工程院校 985工程院校 教育部直属院校

【文章页数】：160 页

【学位级别】：博士

【文章目录】：
Thesis Release Certificate
Certification
ACKNOWLEDGEMENTS
LIST OF TABLES
LIST OF FIGURES
ABBREVIATIONS
Abstract
摘要
CHAPTER 1
    1.1 What is oilseed rape
    1.2 Economic importance
    1.3.Promising heavy metals and their concentration in the soil of ZhejiangProvince
    1.4 Interaction of chromium and plants
    1.5. Interaction of Cr and plants
    1.6. Possible way outs
    1.7. Aims of the study
    1.8. Study outlines
CHAPTER 2
    2.1 What is stress
    2.2 Cr as a toxic and non essential element
        2.2.1 Occurrence
        2.2.2 Cr species
    2.3 Human health and Cr toxicity
    2.4 Hazardous effects of Cr on plants
    2.5 Cr and Brassica napus interaction
    2.6 SA as an elevator
    2.7 Role of GSH to improve the stress impacts on plants
CHAPTER 3
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Plant material and experimental conditions
        3.2.2 Measurement of Plant physio-morphic attributes
        3.2.3 Determination of MDA and singlet oxygen species
        3.2.4 Assessment of enzyme based plant immune system
        3.2.5 Ultrastructural observations
        3.2.6 Statistical analysis
    3.3 Results
        3.3.1 Analyses of physiological parameters
        3.3.2 Determination of Cr and chlorophyll contents
        3.3.3 MDA and ROS conten estimation
        3.3.4 Evaluation of defense related enzymes and TSP contents
        3.3.5 TEM ultrastructural observations of leaf
        3.3.6 TEM ultrastructural observations of root
    3.4 Discussion
    3.5 Conclusions
CHAPTER 4
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Plant material and Growth conditions
        4.2.2 Morphological parameters
        4.2.3 Analysis of lipid peroxidation and reactive oxygen species（ROS）
        4.2.4 Biochemical analysis
        4.2.5 Analyses of detoxification related antioxidants
        4.2.6 Transmission electron microscopy
        4.2.7 Statistical analysis
    4.3 Results
        4.3.1. Morphological attributes
        4.3.2 Photosynthetic gas exchange capacity
        4.3.3. Estimation of MDA and ROS
        4.3.4. Analysis of enzymatic antioxidants and TSP
        4.3.5. Analysis of detoxification related defense mechanism
        4.3.6. TEM cell structural observations
    4.4 Discussion
    4.5 Conclusions
CHAPTER 5
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Plant material and growth conditions
        5.2.2 Chlorophyll and Cr content determination
        5.2.3 Extraction and estimation of total SA contents
        5.2.4 Determination of MDA contents and ROS activity
        5.2.5 Determin ation of antioxidant machinery
        5.2.6 Total RNA extraction,cDNA synthesis,and quantitative real-time PCR assay
        5.2.7 Ultrastructural observations
        5.2.8 Statistical analysis
    5.3 Results
        5.3.1. Morpho-physiological attributes
        5.3.2. Lipid peroxidation and ROS determination
        5.3.3. Antioxidants enzyme activities
        5.3.4. Enzymatic antioxidants transcript level
        5.3.5. Chromium and total salicylic acid contents
        5.3.6. Detoxification and secondary metabolite related gene expressions
        5.3.7. Cell ultra-structural observations
    5.4 Discussion
    5.5 Conclusions
CHAPTER 6
    6.1 Introduction
    6.2 Materials and methods
        6.2.1 Plant material and growth conditions
        6.2.2. Cr and GSH content determination
        6.2.3 Total RNA extraction,reliability assessment and RNA-sequence analyses
        6.2.4 Establishment of de-novo assembly
        6.2.5. Screening of differentially expressed genes（DEGs） and group differentially expressed genes
        6.2.6. Expression pattern analysis of DEGs
        6.2.7. Gene Ontology（GO）functional enrichment analysis（WEGO）of DEGs
        6.2.8. KEGG pathway enrichment analysis
        6.2.9. Satistical analysis
    6.3 Results
        6.3.1. Plant biomass,Cr and GSH contents determination
        6.3.2. RNA sequencing and denovo assembly
        6.3.3. Screening,expression pattern analysis and clustering of differentially expressed genes（DEGs）
        6.3.4. Gene ontology functional classification（WEGO）of DEGs and cluster of orthologous groups（COGs）classification
        6.3.5. KEGG metabolic pathway enrichment analysis
        6.3.6. Analysis of top 10 up-regulated stress response-related DEGs
    6.4 Discussion
    6.5 Conclusions
CHAPTER 7
    7.1 Major findings
    7.2 Future perspectives
References
List of Publications



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