复方中草药提纯剂对宁夏鸡滑液囊支原体病治疗效果的研究

发布时间：2021-07-08 23:50

　　鸡滑液囊支原体病是一种急慢性传染病,其特点是造成鸡、鸭、鹅与野生鸟类渗出性滑膜囊炎和腱鞘炎。根据中国相关法律法规规定对动物的疾病防治过程中不能随意滥用抗生素。而中草药是天然草药制成的添加剂且它的应用为人们提供了更高质量的畜产品。鉴于鸡滑液囊支原体的风险和中草药应用的重要性,本研究旨在探讨不同中草药对宁夏株鸡滑液囊支原体的影响。为测定相关中草药的抑菌效果,选取12种中药提取物对MS（鸡滑液囊支原体）进行了药敏试验,根据结果选取其中五种（大黄、黄连、金银花、党参、甘草）并组合成中药复方制剂。本实验采用微量稀释法（最小抑菌浓度）用无菌蜗牛试管法和牛津杯法进行验证。结果表明,采用微量稀释法,MS对大黄和黄连的敏感性最高,抑菌范围为15.89～16.98 mg/ml,其次是金银花抑菌范围为25.86 mg/ml,党参和甘草抑菌范围为27.69-33.48 mg/ml。采用牛津杯法时,抑菌圈直径为24 mm用S均值表示,14～24 mm的抑菌直径用I均值表示,14 mm以下的抑菌直径用不敏感或抗性的R表示,得到结果大黄、黄连、金银花、党参、甘草抑菌圈均为S。对选取的五种中药大黄、黄连、金银花、党参... 

【文章来源】：宁夏大学宁夏回族自治区 211工程院校

【文章页数】：164 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Main Abbreviation
CHAPTER Ⅰ Introduction
    1. REVEW OF LITRATURE
        1.1 Medicinal plants
        1.2 History and evolution of Class Mollicutes
        1.3 Evolution and phylogeny
        1.4 Biology of Class Mollicutes
            1.4.1 Lack of cell wall
            1.4.2. Small genome
            1.4.3 Scavenging metabolism
        1.5 Avian Mycoplasmosis
            1.5.1 Etiology
        1.6 Mycoplasma Synoviae
            1.6.1 Morphology
            1.6.2 Isolation and Culture
            1.6.3 Physiological and Biochemical Characteristics
            1.6.4 Pathogenic mechanism of Mycoplasma Synoviae
            1.6.5 Resistance
            1.6.6 Epidemiology
        1.7 Clinical symptoms, pathological changes and histological changes
            1.7.1     Pathological changes
            1.7.2 Histological changes
            1.7.3 Diagnosis
            1.7.4 Molecular Biological Detection
        1.8 Prevention and control
            1.8.1 Drug Treatment
            1.8.2 Immunization
CHAPTER Ⅱ Extraction and sensitivity test of Chinese herbs
    2.1.Materials and methods of Chinese herbs extraction
        2.1.1 Reflux extraction method
        2.1.2 Impregnation method
        2.1.3 Reflux heat extraction method
        2.1.4 Advantages and disadvantages of Soxhlet extraction method
    2.2 Drug sensitivity test
    2.3 Results
        2.3.1 Drug sensitivity test of MIC
        2.3.2 Oxford cup method sensitivity test results
    2.4 Discussion
CHAPTER Ⅲ High performance liquid chromatography of Chinese herbs
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Chinese rhubarb(大黄)
        3.2.2 Codonopsis pilosula(党参)
        3.2.3 Honeysuckle(金银花)
        3.2.4 Licorice(甘草)
        3.2.5 Coptis chinensis(黄连)
    3.3 Results
        3.3.1 Calibration results of common peaks in five herbs
    3.4 Discussion
CHAPTER Ⅳ Herbal extracts safety test in mice
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Drug preparation for safety test
        4.2.2 Acute toxicity test of mice by intragastric administration
        4.2.3     Acute toxicity test in mice by intraperitoneal injection(pre-experimental dosagecalculation)
        4.2.4 Acute toxicity test of mice
    4.3 Drug safety results
        4.3.1 Acute toxicity test mice by intragastric administration
        4.3.2 Acute Toxicity Test Of Intraperitoneal Injection Of Mice
        4.3.3 Results of acute toxicity test of intraperitoneal injection of mice
    4.4 Discussion
CHAPTER Ⅴ Establishment of pathological model of MS and exploration of infection pathway
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Main Instruments
        5.2.2 Main Reagents
        5.2.3 Strain
        5.2.4 Preparation of Medium
        5.2.5 Bacterial Solution
        5.2.6 Grouping Treatment of birds chicken
        5.2.7 Treatment of Disease Material Samples and Identification of Mycoplasma in ChickenSynovial Sap
        5.2.8 Detection of serum antibodies against Mycoplasma Synoviae
    5.3 Result and Analysis
        5.3.1 Clinical symptoms
        5.3.2 Pathological examination
        5.3.3 Colony growth of Mycoplasma Synoviae
        5.3.4 Body Temperature Trend Map
        5.3.5 Identification of Mycoplasma in Chicken Synovial Sap by Specific Pohmerase ChainReaction
        5.3.6 Serum Antibody Test
    5.4 Discussion
CHAPTER Ⅵ Chicken pathological model
    6.1 Materials and Methods
        6.1.1 PCR procedure
        6.1.2 Amplification and identification of ms vlha specific gene pcr
    6.2 Protocol of ELISA
        6.2.1 Materials
        6.2.2 Procedure
    6.3 Results
        6.3.1 Chicken model infection results
        6.3.2 Chicken model result after supplementation of herbal extracts
        6.3.3 ELISA results
    6.4 Discussion
CHAPTER Ⅶ Intestinal Histomorphology of chicken after supplementation of compoundherbal medicine extracts
    7.1 Introduction
    7.2 Materials and Methods
    7.3 Results
    7.4 Duodenum
        7.4.1 Villus height(μm)
        7.4.2 Crypt depth(μm)
        7.4.3 Villus surface area(mm~2)
    7.5 Jejunum
        7.5.1 Villus height(μm)
        7.5.2 Crypt depth(μm)
        7.5.3 Villus surface area(mm~2)
    7.6 Ileum
        7.6.1 Villus height(μm)
        7.6.2 Crypt depth(μm)
        7.6.3 Villus surface area(mm~2)
    7.7 Discussion
CHAPTER Ⅷ Haematology and digestibility of chicken after supplementation ofcompound Chinese herbal medicine
    8.1 Methdology
    8.2 Hematology Material and Methods
        8.2.1 Packed cell volume(PCV)
        8.2.2 Hemoglobin (Hb) determination
        8.2.3 Total Leukocyte (WBC) count
        8.2.4 Red Cell (RBC) Count
    8.3 Digestibility trial materials and methods
        8.3.1 Proximate composition of feed and droppings
        8.3.2 Moisture and dry matter contents
        8.3.3 Crude protein
        8.3.4 Ether extract
        8.3.5 Crude fiber
    8.4 Results
        8.4.1 Result of hematology
        8.4.2 Result of digestibility trial
    8.5 Discussion
CHAPTER Ⅸ Effects of Compound Chinese Herbal extracts on Immune organs and Performance of chicken
    9.1 Introduction
    9.2 Materials and methods
    9.3 Results
    9.4 Discussion
Conclusion
References
ACKNOWLEDGEMENTS
DEDICATION



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