The Molecular Ecological Mechanism of Stereoselective Behavi
发布时间：2021-08-05 23:20
在环境化学领域,手性农药的环境行为是研究的热点。超过30%的农药属于手性化合物。研究手性农药的环境行为有助于我们理解手性农药在环境中的进程以及更加全面的评价。本研究选取来自中国不同地区的三种土壤（北京的碱性土壤,长春的中性土壤,武汉的酸性土壤）来研究手性农药三唑酮在这三种不同类型土壤中对映体转化及选择性降解的分子生态机制。主要结果如下:1)手性农药三唑酮对土壤微生物群落的影响。本文将手性农药三唑酮的外消旋体（Rac）,光学纯异构体R（R）和S（S）三种不同形式的化合物添加于三种土壤中并评价微生物群体结构的变化。利用宏基因组学（高通量测序技术）检查和确定微生物群落结构的变化。结果表明三种空白处理的土壤微生物群落结构不同,土壤微生物的物种丰富度和多样性大小为:北京>长春>武汉。此外,长春土壤的主要微生物属于Firmicutes,北京土壤的优势微生物种群是Actinobacteria,Proteobacteria和Acidobacteria是武汉土壤的优势微生物种群。经过R,S和Rac处理的土壤中鉴定到的大部分优势细菌种群（从门到属）表现出明显的降低。大部分Proteobacte...
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：104 页
【学位级别】：博士
【文章目录】：
Abstract
摘要
Chapter 1 LITERATURE REVIEW
1.1 Background
1.2 Triazole Fungicide
1.2.1 Triadimefon
1.2.2 Ecotoxicity of triadimefon
1.3 Chiral resolution of triazole pesticides
1.3.1 Chiral HPLC separation of triazole pesticides
1.3.2 The chiral CE separation of triazole pesticides
1.3.3 Chiral SFC and GC separation of triazole pesticides
1.4 Study on environmental behavior of triazole pesticides
1.4.1 Half-life study
1.4.2 Study on the selective degradation of enantiomers
1.4.3 Photochemical and optical physics research and others
1.5 Soil micro-flora
1.5.1 Distribution of the microbial communities in the surface and subsurface soil
1.5.2 The bacterial communities in the soil
1.6 Measuring the impact of the residual pesticides on the different microbial communities in the soil
1.6.1 Metagenomics
1.6.2 DNA sequences analysis
1.7 Objectives and significances of the study
1.7.1 The main objectives of this study
1.7.2 The significances of this study
Chapter 2 Materials and methods
2.1 Materials
2.1.1 Chemicals and reagents
2.1.2 Soils collection and preparation
2.2 Methods
2.2.1 Experimental design
2.2.2 Enantiomerization and degradation method
2.2.3 High throughput sequences technology
Chapter 3 The impact of the chiral triadimefon compounds on bacterial communities in different types of soils
3.1 Introduction
3.2 Material and Method
3.2.1 Chemical and soils collection and preparation
3.2.2 Experimental design
3.2.3 High throughput sequences technology
3.3 Results and discussion
3.3.1 Sequencing and bacterial diversity indices
3.3.2 Microbial community comparisons
3.3.3 OTU distribution under different TF compounds treatments
3.3.4 Bacteria community structure in blank soils
3.3.5 Bacteria community structure in TFs contaminated soils
3.4 The relationship between microbial communities and environmental Parameters
3.5 Conclusions
Chapter 4 The influence of microbial communities for triadimefon enantiomerization in Soils
4.1 Introduction
4.2 Material and Methods
4.2.1 Chemical and soils collection and preparation
4.2.2 Experimental design
4.2.3 Extraction methods
4.2.4 Analytical procedure
4.2.5 Statistical analysis
4.2.6 High-throughput sequencing
4.3 Results and discussion
4.3.1 Enantiomerization of Triadimefon in different soils
4.3.2 OTUs and Bacterial Community Species Diversity
4.3.3 Variation in Bacterial Community Composition
4.3.4 Correlations between major bacterial groups and triadimefon enantiomerization
4.3.5 Correlations between Major Bacterial Groups and Environmental Factors
4.4 Conclusion
Chapter 5 The influence of microbial communities on the degradation of triadimefon racemate compound in soils
5.1 Introduction
5.2 Material and methods
5.2.1 Chemical and soils collection and preparation
5.2.2 Experimental design
5.2.3 Extraction methods
5.2.4 Analytical procedure
5.2.5 Statistical analysis
5.2.6 High-throughput sequencing
5.3 Results and discussion
5.3.1 Enantioselective and Kinetics Degradation of racemic triadimefon in different soils
5.3.2 Richness and diversity of microbial communities
5.3.3 Variation in bacterial community composition
5.3.4 OTU distribution under different TF compounds treatments
5.3.5 Certain bacterial taxa related to TF-Rac degradation
5.3.6 Correlations between major bacterial groups and environmental factors
5.4 Conclusion
Chapter 6 Summary and perspective work
References
Funding supports
Appendices
Acknowledgement
【参考文献】：
期刊论文
[1]三唑农药的手性拆分及对映体的转化[J]. 李朝阳,张艳川,李巧玲,王未肖,李景印. 分析化学. 2010(02)
[2]Pesticide soil contamination mainly affects earthworm male reproductive parameters[J]. Eduardo Bustos-Obregón,RogerIziga Goicochea. Asian Journal of Andrology. 2002(03)
本文编号：3324625
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：104 页
【学位级别】：博士
【文章目录】：
Abstract
摘要
Chapter 1 LITERATURE REVIEW
1.1 Background
1.2 Triazole Fungicide
1.2.1 Triadimefon
1.2.2 Ecotoxicity of triadimefon
1.3 Chiral resolution of triazole pesticides
1.3.1 Chiral HPLC separation of triazole pesticides
1.3.2 The chiral CE separation of triazole pesticides
1.3.3 Chiral SFC and GC separation of triazole pesticides
1.4 Study on environmental behavior of triazole pesticides
1.4.1 Half-life study
1.4.2 Study on the selective degradation of enantiomers
1.4.3 Photochemical and optical physics research and others
1.5 Soil micro-flora
1.5.1 Distribution of the microbial communities in the surface and subsurface soil
1.5.2 The bacterial communities in the soil
1.6 Measuring the impact of the residual pesticides on the different microbial communities in the soil
1.6.1 Metagenomics
1.6.2 DNA sequences analysis
1.7 Objectives and significances of the study
1.7.1 The main objectives of this study
1.7.2 The significances of this study
Chapter 2 Materials and methods
2.1 Materials
2.1.1 Chemicals and reagents
2.1.2 Soils collection and preparation
2.2 Methods
2.2.1 Experimental design
2.2.2 Enantiomerization and degradation method
2.2.3 High throughput sequences technology
Chapter 3 The impact of the chiral triadimefon compounds on bacterial communities in different types of soils
3.1 Introduction
3.2 Material and Method
3.2.1 Chemical and soils collection and preparation
3.2.2 Experimental design
3.2.3 High throughput sequences technology
3.3 Results and discussion
3.3.1 Sequencing and bacterial diversity indices
3.3.2 Microbial community comparisons
3.3.3 OTU distribution under different TF compounds treatments
3.3.4 Bacteria community structure in blank soils
3.3.5 Bacteria community structure in TFs contaminated soils
3.4 The relationship between microbial communities and environmental Parameters
3.5 Conclusions
Chapter 4 The influence of microbial communities for triadimefon enantiomerization in Soils
4.1 Introduction
4.2 Material and Methods
4.2.1 Chemical and soils collection and preparation
4.2.2 Experimental design
4.2.3 Extraction methods
4.2.4 Analytical procedure
4.2.5 Statistical analysis
4.2.6 High-throughput sequencing
4.3 Results and discussion
4.3.1 Enantiomerization of Triadimefon in different soils
4.3.2 OTUs and Bacterial Community Species Diversity
4.3.3 Variation in Bacterial Community Composition
4.3.4 Correlations between major bacterial groups and triadimefon enantiomerization
4.3.5 Correlations between Major Bacterial Groups and Environmental Factors
4.4 Conclusion
Chapter 5 The influence of microbial communities on the degradation of triadimefon racemate compound in soils
5.1 Introduction
5.2 Material and methods
5.2.1 Chemical and soils collection and preparation
5.2.2 Experimental design
5.2.3 Extraction methods
5.2.4 Analytical procedure
5.2.5 Statistical analysis
5.2.6 High-throughput sequencing
5.3 Results and discussion
5.3.1 Enantioselective and Kinetics Degradation of racemic triadimefon in different soils
5.3.2 Richness and diversity of microbial communities
5.3.3 Variation in bacterial community composition
5.3.4 OTU distribution under different TF compounds treatments
5.3.5 Certain bacterial taxa related to TF-Rac degradation
5.3.6 Correlations between major bacterial groups and environmental factors
5.4 Conclusion
Chapter 6 Summary and perspective work
References
Funding supports
Appendices
Acknowledgement
【参考文献】：
期刊论文
[1]三唑农药的手性拆分及对映体的转化[J]. 李朝阳,张艳川,李巧玲,王未肖,李景印. 分析化学. 2010(02)
[2]Pesticide soil contamination mainly affects earthworm male reproductive parameters[J]. Eduardo Bustos-Obregón,RogerIziga Goicochea. Asian Journal of Andrology. 2002(03)
本文编号：3324625
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