四周低氧训练对Nrf2敲除鼠抗氧化作用的影响

发布时间：2018-05-11 11:59

本文选题：Nrf2敲除鼠 + 低氧运动　；参考：《北京体育大学》2016年博士论文

【摘要】：目的:低氧训练已被广泛用来提高运动员运动能力的训练方法。它试图通过低氧环境和运动双重因素刺激,使机体出现强烈的应激反应,最大程度地调动机体的潜能,从而产生一系列有助于提高运动能力的生理生化反应,以达到提高运动能力的目的。然而,低氧训练对骨骼肌的抗氧化作用分子机制,目前并不十分清楚,低氧训练介导Nrf2对骨骼肌抗氧化作用的影响尚未见报道。因此,本研究通过对Nrf2基因敲除和野生型小鼠进行为期4周的低氧运动训练,观察小鼠骨骼肌ROS含量、SOD和CAT抗氧化酶蛋白表达、GSH/GSSG比值的变化,以及对运动能力的影响,从而为低氧训练的训练效果机制提供一定的理论依据。实验方法:采用Nrf2敲除(KO)和野生(WT)小鼠,分别随机分为两组:常氧安静组和低氧训练组,每组10只。常氧安静组:小鼠在常氧状态下,正常笼中生活,不施加运动负荷;低氧训练组:小鼠每天在常压低氧环境(11.2%的氧浓度)中暴露8小时,并在低氧环境进行坡度为0,运动速度为12米/分跑台运动1小时,每周6天,连续四周。最后一次低氧训练后休息48小时,进行运动能力测定。而后48小时,颈椎脱臼处死,取小鼠骨骼肌,快速置于液氮中,再于-80度冻存。测定骨骼肌ROS、Nrf2mRNA、SOD1、SOD2和CAT蛋白表达、GSH/GSSG比值和运动至力竭的跑距。结果表明(1)野生鼠和Nrf2敲除鼠四周低氧训练后骨骼肌ROS均显著性增加;(2)安静Nrf2敲除鼠Nrf2mRNA、SOD2和CAT蛋白表达量以及运动能力显著低于野生鼠;(3)四周低氧训练后,与野生鼠相比,Nrf2敲除鼠GSH/GSSG比值显著性下降和运动能力非常显著性降低。结论:低氧训练可以提高野生小鼠的抗氧化能力,在Nrf2缺失环境下,低氧训练对Nrf2敲除鼠其无明显改善作用。
[Abstract]:Objective: hypoxia training has been widely used to improve the sports ability of athletes. It tries to stimulate the body by the dual factors of low oxygen environment and movement, and make the body have a strong stress response and maximize the potential of the body, thus producing a series of physiological and biochemical reactions that can help to improve the exercise ability, so as to improve the transportation of the body. However, the molecular mechanism of the antioxidant activity of hypoxia training on skeletal muscle is not very clear. The effect of hypoxia training on the antioxidant effect of Nrf2 on skeletal muscle has not yet been reported. Therefore, this study was conducted to observe the R of skeletal muscle in mice by 4 weeks of hypoxia exercise training in Nrf2 knockout and wild type mice. OS content, the expression of SOD and CAT anti oxidase protein, the change of the GSH/GSSG ratio and the effect on the exercise ability, thus providing a certain theoretical basis for the training effect mechanism of hypoxia training. The experimental method: using Nrf2 knockout (KO) and the wild (WT) mice, they were randomly divided into two groups: normoxic quiet group and hypoxia training group, 10 rats in each group. Quiet group: mice in normal oxygen state, normal cage life, no exercise load, low oxygen training group: mice were exposed to 8 hours in the normal pressure and hypoxia environment (11.2% oxygen concentration) every day, and in the low oxygen environment, the slope was 0, and the speed of movement was 12 meters / sub run 1, 6 days a week, four weeks. After the last hypoxia training, 48 rest 48 rest after 48 rest. For hours, exercise ability was measured. After 48 hours, the cervical dislocations were killed, the skeletal muscles were taken, and the mice were quickly placed in the liquid nitrogen and then stored at -80 degrees. The expression of ROS, Nrf2mRNA, SOD1, SOD2 and CAT protein, the GSH/GSSG ratio and the distance of movement to exhaustion were measured. The results showed (1) the skeletal muscle ROS after the hypoxia training of wild and Nrf2 knockout mice. (2) the Nrf2mRNA, SOD2 and CAT protein expression and exercise ability of quiet Nrf2 knockout mice were significantly lower than those of wild mice; (3) after the four weeks of hypoxia training, the GSH/GSSG ratio of Nrf2 knockout mice decreased significantly and the exercise ability was significantly reduced. Conclusion: hypoxia training can improve the antioxidant capacity of wild mice. In the absence of Nrf2, hypoxic training had no significant effect on Nrf2 knockout mice.

【学位授予单位】：北京体育大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：G804.7

【参考文献】