长期大强度运动对大鼠肠道机能的影响及鼠李糖乳杆菌干预效果

发布时间：2018-10-08 08:35

【摘要】：目的:通过对大鼠进行长期大强度运动和鼠李糖乳杆菌的干预,观察小肠部分益生菌的定植情况,以及反映消化、吸收、屏障等功能的部分指标,发现运动应激与小肠之间的联系,并且探讨益生菌的补充在运动领域中应用的问题。方法:SD大鼠40只随机分为4组。安静营养组和运动营养组每日灌胃鼠李糖乳杆菌溶液2m L。运动对照组和运动营养组在跑台上进行9周的大强度训练。训练最后一周采集大鼠粪便及尿液,测定粪便细菌含量、尿液D-木糖浓度。最后一次训练结束后24小时麻醉处死,测定血清、小肠组织中指标变化。统计学分析采用双因素方差分析。结果:运动对照组血清ACTH水平高于安静对照组(P0.01),安静营养组低于运动营养组(P0.05);运动对照组、安静营养组双歧杆菌低于安静对照组(P0.01);运动对照组小肠组织5-HT水平高于安静对照组(P0.05),血清5-HIAA安静对照组均低于安静营养组和运动对照组(P0.01);安静营养组SERT蛋白表达水平低于安静对照组(P0.05)和运动营养组(P0.05);小肠s Ig A方面,运动营养组和运动对照组均高于安静对照组(P0.01);小肠IL-8方面,运动对照组高于安静对照组(P0.05);血清LPS安静营养组高于安静对照组(P0.05)和运动对照组(P0.01),运动对照组低于运动营养组(P0.05);与运动对照组相比,运动营养组MUC2表达增加(P0.05);安静对照组小肠固有层厚度均小于安静营养组和运动对照组(P0.01);运动对照组小肠绒毛高度小于安静对照组(P0.01)、运动营养组(P0.05),安静营养组的绒毛宽度高于安静对照组(P0.05)。结论:1.本研究采用的9周大强度运动对大鼠造成了一定程度的肠道功能紊乱,体现为定植的双歧杆菌减少,小肠5-HT代谢异常,预示肠道消化功能存在潜在风险。2.大鼠小肠组织的糖转运机制和吸收效率未受到本研究中运动应激的影响,粘膜屏障无明显破坏,未出现病理性变化,但仍存在一定炎症风险。3.研究中所采用的鼠李糖乳杆菌并不能显著改善所出现的功能紊乱,也未影响糖吸收效率和转运机制,并且与运动应激、SERT蛋白表达之间存在相互影响的复杂关系。长期补充会影响小肠粘膜,细菌移位增加,需辩证考虑益生菌的使用。
[Abstract]:Objective: to observe the colonization of some probiotics in the small intestine and some indexes reflecting the function of digestion, absorption and barrier through the long-term intensive exercise and the intervention of Lactobacillus rhamnoides in rats. To explore the relationship between exercise stress and small intestine and to explore the application of probiotic supplement in sports field. Methods 40 Sprague-Dawley rats were randomly divided into 4 groups. Lactobacillus rhamnoides solution was administered daily to rest nutrition group and exercise nutrition group (2m L). The exercise control group and the exercise nutrition group were trained for 9 weeks on the platform. The feces and urine of rats were collected at the end of the training week, and the fecal bacterial content and urine D-xylose concentration were determined. The indexes in serum and small intestine were measured 24 hours after the last training. Two-factor ANOVA was used for statistical analysis. Results: the level of serum ACTH in exercise control group was higher than that in quiet control group (P0.01), and that in quiet nutrition group was lower than that in exercise nutrition group (P0.05). The levels of 5-HT in small intestine in the exercise control group were higher than those in the quiet control group (P0.05), and the levels of serum 5-HIAA in the quiet control group were lower than those in the quiet nutrition group and the exercise control group (P0.01); the expression of SERT protein in the quiet nutrition group was higher than that in the rest nutrition group (P0.01). The level of small intestine s Ig A was lower than that of quiet control group (P0.05) and exercise nutrition group (P0.05). Exercise nutrition group and exercise control group were higher than quiet control group (P0.01), small intestine IL-8, Exercise control group was higher than quiet control group (P0.05), serum LPS quiet nutrition group was higher than quiet control group (P0.05) and exercise control group (P0.01), exercise control group was lower than exercise nutrition group (P0.05); The expression of MUC2 was increased in exercise nutrition group (P0.05), the thickness of lamina propria of small intestine in quiet control group was lower than that in quiet nutrition group and exercise control group (P0.01), the villi height of small intestine in exercise control group was lower than that in quiet control group (P0.01), and that in exercise nutrition group (P0.05). The hair width was higher than that in the quiet control group (P0.05). Conclusion 1. In this study, 9 weeks of intensive exercise caused a certain degree of intestinal dysfunction in rats, which was reflected in the reduction of bifidobacterium colonization and the abnormal metabolism of 5-HT in the small intestine, which indicated that there was a potential risk of intestinal digestive function. The mechanism of glucose transport and absorption efficiency of small intestine in rats were not affected by the exercise stress in this study, the mucosal barrier was not obviously damaged, and no pathological changes occurred, but there was still a certain inflammatory risk. 3. Lactobacillus rhamnoides used in the study did not significantly improve the functional disorder, nor did it affect the glucose absorption efficiency and transport mechanism, and there was a complex relationship between the effects of rhamnobacterium rhamnoides and the expression of SERT protein under exercise stress. Long-term supplementation will affect intestinal mucosa and increase bacterial translocation. The use of probiotics should be considered dialectically.
【学位授予单位】：北京体育大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：G804.2

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