基于MST1探究电针“胰俞”、“脾俞”、“肾俞”干预T2DM的机制

发布时间：2018-04-22 04:31

本文选题：电针 + 2型糖尿病　；参考：《北京中医药大学》2017年博士论文

【摘要】：背景:2型糖尿病(T2DM)发病率、死亡率、经济消耗均较高,给社会和家庭带来了沉重的负担。既往关于T2DM的研究多重视胰岛素抵抗,口服药物也主要以胰岛素增敏剂和促胰岛素分泌剂为主。而在T2DM的发生、发展过程中β细胞功能失调和细胞凋亡起着至关重要的作用,目前尚没有药物能抑制β细胞凋亡和功能衰竭。而抑制MST1可能就是改善β细胞功能失调、抑制β细胞凋亡从而治疗T2DM的新靶点。临床及实验研究均证明针灸可有效干预T2DM。胰俞(胃脘下俞)、脾俞、肾俞是针灸治疗T2DM的常用穴,且三穴均位于胰腺同神经节段。本研究以此为出发点设计实验,观察电针"胰俞"、"脾俞"、"肾俞"干预T2DM动物模型,是否存在疗效差异,并探索电针三背俞穴是否通过MST1相关通路影响β细胞功能和凋亡。本实验聚焦于β细胞功能和β细胞凋亡,吸引大家对于其在T2DM发生发展中作用的重视;同时也更能体现出针灸相较于现有药物通过多途径干预T2DM的优势;MST1是调节β细胞功能和凋亡的多途径通路的上游指标,将研究聚焦于MST1,为针灸干预T2DM的研究提出新的着眼点,同时可从更宏观角度对针刺改善T2DM进行探究。此外,验证了针刺对于MST1的良性调节,可推动针刺对于T1DM疗效和干预机制的研究,从而扩大针灸的应用范围。目的:1.通过检测电针"胰俞"、"脾俞"、"肾俞"对T2DM模型大鼠体质量、空腹血糖(FBG)、口服葡萄糖耐量试验(OGTT)、糖化血清蛋白(GSP)、血脂、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、胰岛β细胞形态的影响,观察电针"胰俞"、"脾俞"、"肾俞"对T2DM的干预效果及疗效差异。2.通过检测电针"胰俞"、"脾俞"、"肾俞"对T2DM模型大鼠胰岛β细胞中MST1基因及蛋白表达、MST1裂解蛋白(clMST1)和磷酸化蛋白(pMST1)、BIM和caspase-3活化、PDX-1蛋白、INS基因和蛋白表达的影响,观察电针"胰俞"、"脾俞"、"肾俞"对T2DM模型大鼠胰岛β细胞凋亡和合成INS功能的影响,探究电针"胰俞"、"脾俞"、"肾俞"干预T2DM的作用机制及差异。方法:将48只雄性SD大鼠随机分为正常组8只和2型糖尿病造模组(简称"造模组")40只。造模组高糖高脂饲料喂养50天的基础上以35mg/kg.BW腹腔注射新鲜配制的链脲佐菌素(STZ)溶液以制造2型糖尿病模型。注射后72h尾静脉采血,以罗氏血糖仪检测随机血糖;注射STZ2周后对大鼠行OGTT。以随机血糖≥16.7mmol/L,OGTT2h后血糖≥11.1mmol/L的大鼠为成功造模大鼠,纳入后续试验。造模成功32只,根据血糖水平随机区组分组,分为模型组8只,电针胰俞组8只,电针脾俞组8只,电针肾俞组8只。各电针组每日干预1次,每次20min,6次/周,连续干预4周。每日观察记录大鼠的一般状况;每周末检测各组大鼠体质量和FBG;干预前后进行OGTT试验,计算曲线下面积。4周后麻醉处死,腹主动脉取血,离心取血清,以自动生化分析仪检测血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、GSP水平,以放免法检测血清INS水平。取部分胰腺组织,一部分多聚甲醛固定,以HE染色观察胰岛形态,并以免疫组化方法检测胰岛β细胞中MST1、clMST1、pMST1、BIM、caspase-3活化、PDX1、INS蛋白的表达水平;一部分液氮冻存,以Real-time PCR法检测大鼠胰腺组织中目的基因MST1及INS的表达水平。结果:1.血糖代谢相关指标:本实验结果显示:在干预4周期间,模型组大鼠体质量基本呈下降态势,4周后较干预前明显降低;而FBG一直维持在较高水平,4周后较干预前显著升高;GSP水平也显示其在过去2-3周内血糖维持在较正常组明显升高的状态;OGTT结果显示其在干预前后均较正常组葡萄糖耐量显著受损,且干预4周后OGTT曲线下面积较自身干预前显著升高,与FBG变化趋势一致;干预4周后HOMA-IR也显著高于正常组;总体而言,说明了干预期4周后模型组大鼠血糖升高,葡萄糖耐量受损和胰岛素抵抗情况恶化,T2DM有所进展。而电针胰俞组大鼠,在4周干预期间,体质量变化趋势同模型组一致,同时间点比较均没有显著差异;FBG较自身而言,在干预第1周末有显著下降,但在干预第2周末又有所回升,此后一直维持在较高水平;但与同时期模型组相比,电针胰俞组在干预第3、4周末FBG水平显著下降;GSP水平也显示其在过去2-3周内血糖平均水平较模型组是显著下降的;OGTT结果同FBG变化趋势一样,干预前后自身无明显变化,但在干预4周后曲线下面积显著低于模型组;干预4周后,电针胰俞组HOMA-IR较模型组显著下降;总体而言,电针胰俞穴干预T2DM模型大鼠4周,可显著抑制血糖升高,改善葡萄糖耐量受损和胰岛素抵抗状态的恶化,抑制T2DM的发展。电针脾俞组大鼠,在4周干预期间,体质量变化趋势同模型组、电针胰俞组一致,同时间点比较均没有显著差异;FBG较自身而言,在干预第1、2周末较干预前显著下降,但干预第3、4周又显著回升;与同时期模型组相比,干预每周末血糖水平均有下降,但差异不显著;GSP水平显示其在过去2-3周内血糖平均水平较模型组是显著下降的;OGTT结果同FBG变化趋势一样,干预前后自身无明显变化,干预4周后曲线下面积比模型组有所缩减,但差异不显著;干预4周后,电针脾俞组HOMA-IR较模型组显著下降;总体而言,电针脾俞穴干预T2DM模型大鼠4周,还是可以改善大鼠血糖升高和葡萄糖耐量受损、胰岛素抵抗状态恶化趋势的,只是不如电针胰俞穴显著。电针肾俞组大鼠,在4周干预期间,体质量变化趋势同模型组、电针胰俞组及电针脾俞组一致,同时间点比较均没有显著差异;FBG较自身而言,在干预第1周末有显著下降,但在干预第2周末又有所回升,此后一直维持在较高水平;与同时期模型组相比,电针肾俞组在干预第3、4周末FBG水平下降,但差异不显著;其在第2周末的FBG水平显著高于电针脾俞组,说明在第2周末时电针脾俞穴降血糖作用优于电针肾俞穴;GSP水平显示其在过去2-3周内血糖平均水平也较模型组是显著下降的;OGTT结果同FBG变化趋势一样,干预前后自身无明显变化,干预4周后曲线下面积比模型组有所缩减,但差异不显著;干预4周后,电针肾俞组HOMA-IR较模型组显著下降;总体而言,电针肾俞穴干预T2DM模型大鼠4周,同电针脾俞穴相似,还是可以改善大鼠血糖升高和葡萄糖耐量受损、胰岛素抵抗状态恶化趋势的,只是不如电针胰俞穴显著。2.血脂相关指标:本实验结果显示:T2DM模型组大鼠TC、TG、LDL水平均较正常组大鼠显著升高,而HDL水平与正常组大鼠无明显变化。这符合HFD/STZ造模大鼠的血脂代谢紊乱特点。电针胰俞穴干预T2DM模型大鼠4周,可降低其TC、TG和LDL水平,改善脂质代谢。电针脾俞穴干预T2DM模型大鼠4周,可降低其LDL水平,而对TC、TG、HDL水平无显著影响。电针肾俞穴干预T2DM模型大鼠4周,可降低TC和LDL水平,而对TG、HDL水平无显著影响。电针胰俞穴调节TG的能力优于电针脾俞穴;电针胰俞调节血脂紊乱的能力比电针脾俞穴和电针肾俞穴更为显著;这也可能是电针胰俞穴调节血糖作用比电针脾俞穴和电针肾俞穴更为显著的原因之一。3.胰岛素水平:本实验结果显示:模型组大鼠胰岛素水平与正常组无显著差异,而电针胰俞穴可显著降低T2DM模型大鼠胰岛素水平,电针脾俞穴、电针肾俞穴有降低胰岛素水平的趋势,但效果不显著。4.MST1相关机制指标:本实验结果显示:与正常组相比,T2DM模型组大鼠MST1基因和蛋白的表达增多,MST1蛋白的裂解和磷酸化水平升高,即MST1蛋白的活化增多;线粒体凋亡途径中促凋亡因子BIM蛋白和caspase-3裂解蛋白表达增多,即BIM和caspase-3的活化增强,细胞凋亡增多;PDX1蛋白的表达减少,INS基因和蛋白表达减少,即胰岛β细胞合成胰岛素的功能降低。电针胰俞穴、电针脾俞穴均可减少T2DM模型大鼠MST1基因和蛋白的表达,并显著抑制MST1蛋白的裂解和磷酸化,即抑制MST1蛋白的活化;从而进一步显著抑制线粒体凋亡途径中促凋亡因子BIM和caspase-3的活化,产生抑制细胞凋亡的效应。同时电针胰俞穴、电针脾俞穴均可显著促进PDX1蛋白的表达,并促进PDX1启动INS基因的转录表达,促进INS蛋白的表达,即促进胰岛β细胞合成胰岛素的功能。而电针肾俞穴可抑制MST1基因和蛋白的表达,并显著抑制MST1蛋白的裂解,对其磷酸化的影响效果不明显;但也可以进一步显著抑制BIM和caspase-3的活化,产生抑制细胞凋亡的效应。同时电针肾俞穴可显著促进PDX1蛋白的表达,但对于INS基因转录的影响不明显,却可以显著促进INS蛋白的表达,由此也可说电针肾俞穴亦可以促进胰岛β细胞合成胰岛素的功能。由此可见,电针胰俞穴、电针脾俞穴、电针肾俞穴均可以通过调节MST1相关基因和蛋白表达抑制β细胞的凋亡,促进β细胞合成胰岛素的功能。结论:1.电针胰俞穴、电针脾俞穴、电针肾俞穴干预T2DM模型大鼠4周,均可以抑制T2DM大鼠血糖升高、葡萄糖耐量受损、胰岛素抵抗状态恶化的趋势,均可从一定程度上改善血脂代谢;但电针脾俞穴和电针肾俞穴调节血糖、血脂代谢的能力不如电针胰俞穴显著。由此可见,胰俞在干预治疗T2DM上具有一定的优势和特异性。鉴于三穴均位于胰腺同神经节段内,但又与胰腺有不同节段联系,还不能确定电针胰俞、脾俞、肾俞干预T2DM起效的机制及疗效的差异与神经节段无关,但具体关系还有待进一步的探究。2.电针胰俞穴、电针脾俞穴、电针肾俞穴干预T2DM模型大鼠4周,均可通过抑制MST1基因和蛋白的表达,以及抑制MST1的活化,抑制β细胞凋亡,促进β细胞合成INS能力,进而发挥调节血糖、血脂代谢的作用。电针胰俞穴、电针脾俞穴、电针肾俞穴对机制指标影响无明显差异。胰俞干预T2DM的特异性机制还有待进一步探索。
[Abstract]:Background: the incidence, mortality and economic consumption of type 2 diabetes mellitus (T2DM) are all high, bringing heavy burden to society and family. Previous studies on T2DM pay more attention to insulin resistance. Oral drugs are mainly insulin sensitizer and insulin secretant. In the development of T2DM, the development process of beta cell dysfunction and cells Apoptosis plays a vital role. At present, there is no drug to inhibit apoptosis and failure of beta cells. Inhibition of MST1 may be a new target for the improvement of beta cell dysfunction, inhibition of beta cell apoptosis and the treatment of T2DM. Clinical and experimental studies have proved that acupuncture can effectively interfere with T2DM. Yishu (xiishu), spleen Yu, and Shenshu is the treatment of acupuncture and moxibustion. The common acupoints for the treatment of T2DM were located in the same ganglion segment of the pancreas. This study was designed as the starting point for the experiment to observe the effect of Electroacupuncture on "Yishu", "spleen Yu" and "Shenshu" in the T2DM animal model, whether there was a difference in effect and whether the three back Shu Points of electroacupuncture could affect the function and apoptosis of beta cells through the MST1 related pathway. The experiment focused on the beta cells. Function and beta cell apoptosis attract people's attention to its role in the development of T2DM; meanwhile, it can also reflect the advantages of acupuncture and moxibustion compared with existing drugs to intervene in T2DM through multiple pathways; MST1 is the upstream index for regulating the function and apoptosis of beta cells, focusing on MST1, and putting forward a new study on the intervention of acupuncture and moxibustion in T2DM. At the same time, we can explore the improvement of T2DM by acupuncture at a more macro point of view. Furthermore, it proves that acupuncture has a good regulation of MST1, which can promote the effect of acupuncture on the effect of T1DM and the mechanism of intervention, so as to enlarge the application scope of acupuncture and moxibustion. Objective: 1. the body mass of T2DM model rats was detected by the detection of electroacupuncture "Yu Yu", "spleen Yu" and "Shenshu". Blood glucose (FBG), oral glucose tolerance test (OGTT), glycosylated serum protein (GSP), blood lipid, fasting insulin (FINS), insulin resistance index (HOMA-IR), the morphology of islet beta cells, observation of the effect and effect difference of Electroacupuncture of "Yu Yu", "spleen Yu", "Shenshu" on T2DM and the difference of curative effect.2. through the detection of electroacupuncture "Yu Yu", "spleen Yu", "Shenshu" "T2DM model big. The effects of MST1 gene and protein expression, MST1 lysis protein (clMST1) and phosphorylated protein (pMST1), BIM and caspase-3 activation, PDX-1 protein, INS gene and protein expression, and the effect of Electroacupuncture on the apoptosis of pancreatic beta cells and the function of INS in T2DM model rats were observed, and the Electroacupuncture of "Yu Yu" and "spleen Yu" were investigated. The mechanism and difference of the effect of "Shenshu" on the intervention of T2DM. Methods: 48 male SD rats were randomly divided into 8 rats in the normal group and 40 in type 2 diabetes model ("model group"). On the basis of high sugar and high fat feed for 50 days, a new type of streptozotocin (STZ) solution was injected into 35mg/kg.BW to produce type 2 diabetes model. After 72h tail vein blood sampling, random blood sugar was detected by Roche blood glucose meter. Rats with OGTT. with random blood glucose of more than 16.7mmol/L and OGTT2h after OGTT2h after STZ2 weeks were successful model rats, and the model group was successfully established. The model group was divided into 8 rats in the model group and 8 rats in the electroacupuncture and the Yu group. Acupuncture and spleen Yu Group 8, electroacupuncture Shenshu Group 8. Each acupuncture group intervention 1 times a day, each time 20min, 6 times per week, continuous intervention for 4 weeks. Observe and record the general condition of rats daily; test each group of rats' body mass and FBG every weekend; before and after the intervention, the OGTT test, the calculation of the area under the curve for.4 weeks after anesthesia, the abdominal aorta blood, centrifugation serum, in order to take the serum, in order to centrifuge the serum. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), GSP level, serum INS level were detected by radioimmunoassay analyzer. Partial pancreatic tissue, partial paraformaldehyde was fixed, HE staining was used to observe the form of islet, and MST1, clMST1, pMST in islet beta cells were detected by immunohistochemical method. 1, BIM, caspase-3 activation, PDX1, INS protein expression level; a part of liquid nitrogen cryopreservation, Real-time PCR method to detect the expression level of MST1 and INS in rat pancreas tissue. Results: 1. blood glucose metabolism related indicators: the results of this experiment showed that during the 4 week intervention, the body mass of the model group decreased basically, 4 weeks later than before the intervention. The level of FBG maintained at a higher level and increased significantly after 4 weeks than before the intervention; the GSP level also showed that the blood glucose level in the last 2-3 weeks was significantly higher than that in the normal group; the OGTT results showed that the glucose tolerance was significantly impaired before and after the intervention, and the area under the OGTT curve was more than before the intervention for 4 weeks. In 4 weeks, HOMA-IR was significantly higher than that in the normal group, and after 4 weeks of intervention, HOMA-IR was also significantly higher than that of the normal group. In general, it was indicated that the blood glucose of the rats in the model group, the impaired glucose tolerance and the deterioration of insulin resistance, and the progress of T2DM in the model group after the dry expectation, and the body mass change during the 4 week intervention of the electroacupuncture and pancreas Yu group were the same as that of the model group. There was no significant difference at the same time point; FBG was significantly lower at the first weekend than the intervention, but it rose again at the end of the second week, and then maintained at a higher level, but compared with the same period model group, the level of FBG decreased significantly at the end of the intervention on the weekend of 3,4; the GSP level also showed that it was in the past 2-3 weeks. The average blood sugar level was significantly lower than that in the model group; the OGTT result was the same as the FBG change trend, but the area under the curve was significantly lower than that in the model group after 4 weeks of intervention, and after the intervention for 4 weeks, the HOMA-IR of the electroacupuncture and pancreas Yu group was significantly lower than that of the model group. In general, the electroacupuncture and Yishu acupoint intervention for the T2DM model rats was significant for 4 weeks. Inhibit the increase of blood glucose, improve the deterioration of glucose tolerance and insulin resistance, and inhibit the development of T2DM. The body mass changes during the 4 week intervention were the same as that in the model group, the electroacupuncture and the Yishu group, and there was no significant difference at the same time point; FBG was significantly lower than the intervention at the end of the intervention for the week 1,2. In the same period, the average blood glucose water decreased, but the difference was not significant compared with the same period model group, but the GSP level showed that the blood glucose level in the last 2-3 weeks was significantly lower than that in the model group; the OGTT result was the same with the FBG, and there was no obvious change before and after the intervention, and the curve after the intervention was 4 weeks after the intervention. The lower area was less than that of the model group, but the difference was not significant. After 4 weeks of intervention, the HOMA-IR was significantly lower than that of the model group. As a whole, the intervention of the T2DM model rats by the acupuncture at the acupuncture point of the spleen and the spleen can improve the increase of blood glucose and glucose tolerance in rats, and the tendency of insulin resistance to deteriorate is not as good as that of the electroacupuncture Yu Xuexian. In the 4 week intervention, the trend of body mass change was the same as that of the model group, the Electroacupuncture of the Yu Yu group and the electroacupuncture spleen Yu group, and there was no significant difference at the same time point. FBG compared with itself, there was a significant decrease at the end of the intervention for the first weekend, but after the intervention of the second weekend, it remained at a higher level; and the same period. Compared with the model group, the level of FBG in the electroacupuncture Shenshu group decreased at the end of the 3,4 weekend, but the difference was not significant. The level of FBG in the second weekend was significantly higher than that of the electroacupuncture spleen Yu group. It showed that the hypoglycemic effect of the acupuncture point at the second weekend was better than that of the electroacupuncture on the Shenshu Point. The level of GSP showed that the average level of blood glucose in the past 2-3 weeks was significantly lower than that of the model group. The OGTT results were the same as the FBG change trend, and there was no obvious change before and after intervention. After 4 weeks, the area under the curve was reduced, but the difference was not significant. After 4 weeks, the HOMA-IR of the electroacupuncture Shenshu group was significantly lower than that of the model group; in general, the electroacupuncture Shenshu acupoint was similar to the Electroacupuncture of the splenic Shu point for 4 weeks. In order to improve the increase of blood glucose and impaired glucose tolerance in rats, the tendency of insulin resistance to deteriorate is not as good as the significant.2..2. blood lipid related indexes of electroacupuncture and pancreas: the results of this experiment show that the level of TC, TG and LDL in the T2DM model group is significantly higher than that of the normal group, but the HDL level has no obvious change with the normal group. This conforms to the HFD/STZ made. The intervention of T2DM model rats with electroacupuncture at 4 weeks can reduce the level of TC, TG and LDL and improve the lipid metabolism. The interference of the T2DM model rats by the acupuncture of the electroacupuncture spleen Yu point for 4 weeks can reduce the level of LDL, but has no significant influence on the level of TC, TG and HDL. The intervention of the Shenshu Point in the T2DM model rats for 4 weeks can reduce TC and LDL levels. There was no significant influence on the level of TG and HDL. The ability of electroacupuncture at Yishu point to regulate TG was better than that of the Electroacupuncture of spleen Yu Xue, and the ability of electroacupuncture and pancreas Yu to regulate blood lipid disorders was more significant than that of the acupuncture point of the electroacupuncture spleen and the electroacupuncture of Shenshu. It may also be one of the more significant reasons for the regulation of blood glucose in the Electroacupuncture of the pancreas and the pancreas, which is one of the more significant reasons for the.3. insulin water than the electroacupuncture at the spleen and the electroacupuncture at the Shenshu Point. The results of this experiment showed that the insulin level in the model group was not significantly different from that in the normal group, but the electroacupuncture and pancreatic Yu Xueke significantly reduced the insulin level in the T2DM model rats, the electroacupuncture at the spleen Yu point and the electroacupuncture at Shenshu Point had a tendency to reduce the insulin level, but the effect was not significant in.4.MST1 phase. The results of this experiment showed that compared with the normal group, the results of this experiment showed that the results of this experiment were compared with that of the normal group. The expression of MST1 gene and protein in the T2DM model group increased, the MST1 protein lysis and phosphorylation level increased, that is, the activation of MST1 protein increased, the apoptosis factor BIM protein and the caspase-3 lysis protein expression increased in mitochondrial apoptosis pathway, that is, the activation of BIM and Caspase-3, the increase of apoptosis, the decrease of PDX1 protein, INS based The decrease of the protein expression and the decrease in the expression of insulin in islet beta cells. The electroacupuncture at the Yishu point and the Electroacupuncture of spleen Yu Xue can reduce the expression of MST1 gene and protein in the T2DM model rats, and significantly inhibit the cracking and phosphorylation of MST1 protein, that is, to inhibit the activation of MST1 protein, and thus further inhibit the apoptosis in mitochondrial apoptosis pathway to further inhibit apoptosis. The activation of factor BIM and caspase-3 produced the effect of inhibiting apoptosis. At the same time, electroacupuncture at Yishu point can promote the expression of PDX1 protein, promote the transcription of INS gene and promote the expression of INS protein, promote the function of islet beta cells in the islet beta cell synthesis, and the electroacupuncture at Shenshu point can inhibit the MST1 gene and the function of the MST1 gene and the electroacupuncture at Shenshu Point. Protein expression, and significantly inhibit the fragmentation of MST1 protein, has no obvious effect on its phosphorylation, but it can further inhibit the activation of BIM and Caspase-3 and inhibit the apoptosis of cells. At the same time, the electroacupuncture at Shenshu Point can significantly promote the expression of PDX1 protein, but the effect on INS gene transcription is not obvious, but it can be significant. The expression of INS protein can be promoted, and it can also be said that electroacupuncture at Shenshu Point can also promote the function of insulin synthesis in islet beta cells. Thus, it can be seen that electroacupuncture at Yishu acupoint, electroacupuncture spleen Yu point, and electroacupuncture at Shenshu Point can inhibit the apoptosis of beta cells by regulating MST1 related genes and protein expression, and promote the function of insulin synthesis in beta cells. Conclusion: 1. electroacupuncture. Yishu acupoint, electroacupuncture spleen Shu point, electroacupuncture at Shenshu Point intervention T2DM model rats for 4 weeks, can inhibit the increase of blood glucose in T2DM rats, the impaired glucose tolerance and the deterioration of insulin resistance, which can improve blood lipid metabolism to a certain extent, but the ability of electroacupuncture at spleen Yu point and Electroacupuncture at Shenshu acupoint to regulate blood glucose and blood lipid metabolism is not as good as that of electroacupuncture Yu Xue It can be seen that Yishu has some advantages and specificity in the intervention treatment of T2DM. Given that three points are all located in the same ganglion segment of the pancreas, but there are different segments associated with the pancreas, the mechanism and the difference of the effect of the intervention of T2DM in the electroacupuncture, the spleen Yu, the Shenshu intervention are not related to the ganglion segment, but the specific relationship remains to be entered. One step to explore.2. electroacupuncture at Yishu point, electroacupuncture spleen Yu point, electroacupuncture at Shenshu Point intervention T2DM model rats for 4 weeks, can inhibit the expression of MST1 gene and protein, and inhibit the activation of MST1, inhibit the apoptosis of beta cells, promote the synthesis of INS in beta cells, and then play the role of regulating blood glucose and lipid metabolism. Electroacupuncture at Yishu point, electric acupuncture spleen Yu Xue, electricity There is no significant difference in the effect of acupuncture Shenshu Point on the mechanism index. The specific mechanism of Yishu intervention on T2DM needs further exploration.

【学位授予单位】：北京中医药大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R245

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