池蝶蚌多糖的生物功能活性分析

发布时间：2018-04-05 14:03

本文选题：多糖　切入点：池蝶蚌　出处：《南昌大学》2017年硕士论文

【摘要】：池蝶蚌(Hyriopsis schlegelii)属于软体动物们,瓣鳃纲,蚌科,帆蚌属,原产于日本,是优质淡水育珠蚌,所产珍珠个体大、圆、光,品质十分优良。1997年从日本引进我国,由于其育珠性能良好、抗病能力强,现被作为优良的经济水产品种推广应用。本实验室对池蝶蚌持续研究20年,在遗传育种、优化培养等方面取得了一定成果,但对其生物学活性物质等方面的研究还较少。池蝶蚌多糖类物质的提取及相关功能活性的研究刚刚起步,本研究旨在对池蝶蚌多糖的生物活性做初步的研究,为池蝶蚌多糖的开发利用提供一定的依据。针对以前的多糖提取方法,本研究调整了池蝶蚌多糖的提取技术,使提取更加方便,得到了池蝶蚌多糖组分HSP-1和HSP-2,提取率分别为1.9%和0.95%;对池蝶蚌多糖的抗肿瘤、免疫及抗氧化相关的活性做了初步的分析探讨。在抗氧化实验中,分别选取DPPH、羟自由基和超氧自由基的清除活性以及还原力作为体外指标;体内抗氧化测定,给D-半乳糖小鼠衰老模型定期灌胃池蝶蚌多糖,发现在小鼠血清中,池蝶蚌多糖(HSP-1)高剂量组的T-SOD酶活力为155U/ml,而阴性对照组血清中则是122 U/ml,MDA阴性对照组含量为13.64nmol/ml,高剂量组的含量为9.37 nmol/ml,总抗氧化活性高剂量组为11.22U/ml,阴性对照组仅为6.48;在肝脏中,MDA阴性对照组含量为21.15 nmol/ml,高剂量组仅为11.30 nmol/ml,阴性对照组的T-SOD活力为71.55U/mg,高剂量组为134.33 U/mg,总抗氧化能力阴性对照组为4.43 U/mg,高剂量组为13.64 U/mg。经过实验结果数据分析比较可以看出HSP-1具有提高抗氧化酶活力及降低氧化产物MDA含量的能力,抗氧化实验结果表明池蝶蚌多糖HSP-1具有较好的抗氧化功能。选取SMMC-7721和SGC-7901两株肿瘤细胞株,用HSP-1和HSP-2进行了抗肿瘤生物活性实验,100 mg/L时HSP-1、HSP-2及可溶性淀粉对SMMC-7721细胞增殖影响效果分别为1.11±0.07、0.98±0.13和1.06±0.06,对于SGC-7901细胞影响为0.94±0.03、1.09±0.08和0.85±0.07,结果显示,两种多糖组分在体外均未表现出明显抑制肿瘤的结果;而另一方面,将池蝶蚌多糖体外作用于小鼠脾脏淋巴细胞时,结果显示其能够使脾脏淋巴细胞增殖,脾脏显著增大,高剂量组脾重指数约为0.358,而阴性对照组的脾重指数约为0.099,因此我们推测池蝶蚌多糖具有提高机体免疫功能的活性。
[Abstract]:Hyriopsis Schlegelii) is a kind of mollusk. It is native to Japan. It is a kind of high-quality freshwater oyster. The pearls produced by Hyriopsis schlegelii are of great size, round, light and excellent quality. In 1997, China was introduced from Japan because of its good bead breeding performance.Strong resistance to disease, is being used as an excellent economic aquatic varieties to promote and use.In our laboratory, we have made some achievements in genetics and breeding, optimization of culture and so on, but there are few studies on biological active substances in this laboratory.The extraction of polysaccharides and their related functional activities have just started. The purpose of this study is to make a preliminary study on the biological activities of polysaccharides from the mussels, and to provide some basis for the development and utilization of polysaccharides.In view of the previous polysaccharide extraction methods, this study adjusted the extraction technology of Polysaccharide from Phellodendron ponyrii to make the extraction more convenient, and obtained polysaccharide components HSP-1 and HSP-2, the extraction rates were 1.9% and 0.95%, respectively.The activities related to immunity and anti-oxidation were preliminarily analyzed and discussed.The scavenging activity and reductive capacity of DPPH, hydroxyl radical and superoxide free radical were selected as the in vitro indexes respectively.It was found that in mouse serum,The activity of T-SOD enzyme was 155U / ml in high dose group, 13.64nmol / ml in negative control group, 9.37nmol / ml in high dose group, 11.22Umlml in high dose group and 6.48nmol / ml in negative control group.The content of T-SOD in liver was 21.15 nmol / ml in negative control group, 11.30 nmol / ml in high dose group, 71.55 U / mg in negative control group, 134.33 U / mg in high dose group, 4.43 U / mg in total antioxidant capacity negative control group and 13.64 U / mg in high dose group.The results showed that HSP-1 could increase the activity of antioxidant enzymes and decrease the content of MDA. The results of antioxidant experiments showed that HSP-1 had better antioxidant function.On the other hand, when the polysaccharides were treated in vitro with the mouse spleen lymphocytes, the results showed that the two polysaccharides could make the spleen lymphocytes proliferate and the spleen increase significantly.The spleen weight index of the high dose group was about 0.358, while that of the negative control group was about 0.099. Therefore, we speculated that the polysaccharide from the mussel had the activity of improving the immune function of the body.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S917.4

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