包被植物精油替代硫酸粘杆菌素对断奶仔猪生长性能和肠道功能的影响

发布时间：2018-04-14 17:38

本文选题：包被植物精油 + 断奶仔猪　；参考：《华中农业大学》2017年硕士论文

【摘要】：试验以不同水平包被植物精油代替日粮中硫酸粘杆菌素,研究对断奶仔猪生长性能、肠道菌群、炎症反应、抗氧化性及肠道屏障功能的影响。试验选用200头,25日龄断奶,体重为7.23±0.75 kg的三元杂断奶仔猪,随机分成5组,每组5栏,每栏8头仔猪,公母各半。对照组饲喂不含硫酸粘杆菌素的基础日粮,抗生素组在基础日粮中添加60 mg/kg硫酸粘杆菌素,精油处理组在基础日粮中添加100、200或300mg/kg包被植物精油,分两阶段进行共35天的饲养试验。在断奶后第7天,从抗生素组、对照组、200 mg/kg包被植物精油组每栏选取1头,进行屠宰和取样。结果如下:(1)日粮中添加100 mg/kg或200 mg/kg包被植物精油可以维持与添加60 mg/kg硫酸粘杆菌素组相当的平均日采食量和日增重,300 mg/kg包被植物精油替代硫粘菌素会显著降低仔猪平均日采食量和日增重(P0.05);(2)日粮中添加包被植物精油或硫酸粘杆菌素能极显著降低仔猪腹泻率(P0.01),添加200、300 mg/kg包被植物精油或60 mg/kg硫酸粘杆菌素能显著降低腹泻评分(P0.05)。(3)与对照组比,添加200 mg/kg包被植物精油或硫酸粘杆菌素能显著降低结肠内容物中大肠杆菌、肠球菌相对表达量(P0.05),对乳酸杆菌相对表达量无显著影响(P0.05)。(4)与对照组比,日粮中添加200 mg/kg包被植物精油能显著提高仔猪绒毛高度和绒腺比(P0.05),升高仔猪空肠粘膜中咬合蛋白Occludin mRNA相对表达量(P0.01),极显著降低断奶后7 d和14 d血浆中D-乳酸含量(P0.01),显著降低断奶后7 d血浆DAO活性(P0.05),极显著降低断奶后14 d血浆DAO活性(P0.01)。(5)与对照组比,日粮中添加200 mg/kg包被植物精油能极显著降低空肠促炎症因子IL-1βmRNA相对表达量(P0.01),显著降低TNF-αmRNA相对表达量(P0.05),显著增加空肠粘膜s IgA浓度(P0.05)。(6)与对照组比,日粮中添加200 mg/kg包被植物精油能显著增加空肠组织匀浆中SOD、GSH-px酶活性,显著降低MDA含量(P0.05)。同时,添加200 mg/kg包被植物精油能极显著降低血浆ROS含量(P0.01)。综上所述,断奶仔猪日粮中添加100或200 mg/kg包被植物精油,可以维持与添加60 mg/kg硫酸粘杆菌素相当的生长性能。添加200 mg/kg包被植物精油可以有效减少肠道病原菌,改善机体的氧化应激和炎症状态,支持肠道屏障功能,减少仔猪腹泻。
[Abstract]:The effects of different levels of coated essential oil on growth performance, intestinal flora, inflammatory reaction, antioxidant activity and intestinal barrier function of weaned piglets were studied.Two hundred weaned piglets of 25 days old, weighing 7.23 卤0.75 kg, were randomly divided into 5 groups with 8 piglets per column and half male and female piglets.The control group was fed a basal diet without mucin sulfate, the antibiotic group added 60 mg/kg mucin sulfate to the basal diet, and the essential oil treatment group added 100200 or 300mg/kg coated plant essential oil to the basic diet.The feeding experiment was conducted in two stages for 35 days.On the 7th day after weaning, one head was selected from each column in the antibiotic group and the control group, which was coated with vegetable essential oil for 200 mg/kg, and then slaughtered and sampled.The results were as follows: (1) supplementation of 100 mg/kg or 200 mg/kg coated essential oils could maintain the average daily intake and daily gain of 100 mg/kg or 200 mg/kg coated plant essential oils instead of thiomycin significantly lower than that of the 60 mg/kg mucin sulfate group.Compared with the control group, the average daily intake and daily gain of pigs were significantly decreased by adding plant essential oil or mucin sulfate to the diet, and the diarrhea rate of piglets was significantly decreased by adding 200300 mg/kg coated vegetable essential oil or 60 mg/kg mucin sulfate.The relative expression of Escherichia coli in colon was significantly decreased by adding 200 mg/kg coated plant essential oil or mucin sulfate. The relative expression of Escherichia coli in Escherichia coli was not affected by the relative expression of Lactobacillus.Dietary supplementation of 200 mg/kg coated plant essential oil significantly increased villus height and chorionic gland ratio (P0.05N), increased the relative expression of Occludin mRNA in jejunal mucosa of piglets and significantly reduced plasma D-lactic acid content at 7 and 14 days after weaning.The plasma DAO activity was significantly decreased at 7 days after weaning, and the plasma DAO activity was significantly decreased at 14 days after weaning compared with the control group.The relative expression of IL-1 尾 mRNA in jejunum and the relative expression of TNF- 伪 mRNA were significantly decreased in diet supplemented with 200 mg/kg coated vegetable essential oil. Compared with the control group, the relative expression of TNF- 伪 mRNA and the concentration of s IgA in jejunum were significantly increased.Dietary supplementation of 200 mg/kg coated essential oil significantly increased the activity of SOD GSH-px in jejunum homogenate and significantly decreased the content of MDA.At the same time, the addition of 200 mg/kg coated plant essential oil could significantly reduce plasma ROS content (P 0.01).In conclusion, the growth performance of weaned piglets fed with 100 or 200 mg/kg coated plant essential oil was comparable to that of 60 mg/kg mucin sulfate supplementation.The addition of 200 mg/kg coated plant essential oil can effectively reduce intestinal pathogens, improve oxidative stress and inflammation, support intestinal barrier function, and reduce diarrhea in piglets.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S828.5

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