利用选择性基因分型技术和残余杂合系群体定位大豆生育期和分枝数相关QTL

发布时间：2018-04-15 01:17

本文选题：大豆 + 生育期　；参考：《聊城大学》2017年硕士论文

【摘要】：生育期和分枝数都是影响大豆栽培措施和产量的重要农艺性状,对影响这两个性状的QTL座位进行定位具有重要的理论和现实意义。为定位生育期相关QTL座位,本研究以栽培大豆菏豆14为母本,野生大豆ZYD02810为父本,构建生育期分离群体。在F_2代,利用选择基因型方法进行生育期初步QTL座位定位,结果表明C2连锁群上Satt307(121.27 cM)和C1连锁群上Satt718(73.79cM)座位与生育期相关。进一步利用F_2群体中筛选出的F_(2:3)晚熟残余杂合株系继续种植分离群体对上述定位结果进行验证。结果表明,对于Satt307座位,无论哪种方法,均能得到验证;而对于Satt718座位只在F_2单尾中得到验证,F_(2:3)晚熟残余杂合株系没有得到验证,其结论尚需进一步验证。另外,通过对已克隆成熟期基因E1~E4测序结果表明,这些基因在亲本间没有差异,表明本研究中的生育期性状与这些基因没有关系。为定位大豆分枝数相关QTL,本研究以寡分枝栽培大豆冀黄13为母本,多分枝地方品种小黑豆为父本配制杂交组合,在2012年徐艳萍利用F_(2:3)群体通过寡分枝单尾法选择性基因分型方法定位分枝数QTL的基础上,在2014,我们又利用F_2:4群体通过双尾法选择性基因分型方法对大豆分枝数进行QTL定位。研究表明2014年,在F_2:4分离群体中的双尾群体中共检测到2个与分枝数QTL座位连锁的座位,分别是F连锁群上的BARC13-1845座位和B2连锁群上的BARC14-1214座位;而在2012年利用寡分枝单尾群体检测到的L连锁群上BARC19-1222(71.32cM)座位,在2014年并没有被检测到。在2014年检测到的两个分枝数QTL座位附近,没有其他分枝数相关QTL座位的报道,这两个座位可能是新的。本研究将为进一步进行分枝数QTL座位的精细定位和分子标记辅助选择育种奠定基础。
[Abstract]:The growth period and branching number are important agronomic characters that affect soybean cultivation measures and yield. It is of great theoretical and practical significance to map the QTL loci which affect the two traits.In order to localize the QTL loci related to growth period, a segregated population was constructed by using cultivated soybean Headou 14 as female parent and wild soybean ZYD02810 as male parent.The preliminary QTL loci were mapped by genotypic selection in the second generation of FSCL. The results showed that Satt307(121.27 cM) on C2 linkage group and Satt7181873.79cM) locus on C1 linkage group were correlated with growth period.The results were further verified by using the late mature residual heterozygous lines, which were selected from the F _ (2) population, and continued to be planted and segregated.The results show that the Satt307 locus can be verified by either method, but the Satt718 locus can only be verified in the single tail of FSC2. The results show that the residual heterozygous lines of late maturing can not be verified, and the conclusion needs further verification.In addition, E1~E4 sequencing of cloned mature genes showed that there was no difference between parents, indicating that the growth stage traits in this study were not related to these genes.In order to locate the QTL-based branch number correlation of soybean, the hybrid combination was prepared by using oligobranched soybean Jihuang 13 as female parent and multi-branched local variety Xiaotou bean as male parent.In 2012, Xu Yanping used Fsta2: 3) to locate the number of branches (QTL) by the method of selective genotyping with oligobranching single tail method. In 2014, we used F2: 4 population to localize the number of branches of soybean by using the method of two-tail selective genotyping.The results showed that in 2014, two loci linked to branched QTL loci were detected in the two-tailed population of FSCL, namely BARC13-1845 locus in F linkage group and BARC14-1214 locus in B2 linkage group.However, no BARC19-122222 71.32cM locus was detected in the L linkage group detected in 2012 using oligobranched single-tailed populations, but was not detected in 2014.There were no reports of other branched QTL loci near the two branched QTL loci detected in 2014, which may be new.This study will lay a foundation for further fine mapping of branch number QTL loci and molecular marker-assisted selection breeding.
【学位授予单位】：聊城大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S565.1

【参考文献】