四种转基因大豆的毒理学评价

发布时间：2018-04-29 10:04

本文选题：转基因 + 大豆　；参考：《延边大学》2015年硕士论文

【摘要】：转基因大豆是世界上最早引入并商业化种植的转基因农作物之一,已成为世界大豆生产的主流品种,而自转基因大豆问世以来,它给社会带来了巨大的经济效益和社会效益,同时其安全性问题也成为人们探讨的焦点,其焦点主要围绕着是否会危害人类健康及破坏生态环境,这就迫切要求我们加快开展关于转基因大豆安全性评价的研究。本文通过毒理学试验评价了转基因大豆的食用安全性。为确定四种转基因大豆对人体的潜在隐患,以灌胃的方式对雌雄小鼠进行了急性毒性试验、以染色体完整性改变和染色体分离改变为遗传学终点的微核试验、反映潜在的致突变性和生殖毒性的精子畸形试验。比较了两种非转基因亲本大豆和四种转基因大豆的临床表现和组织病理学参数。实验结果表明：(1)4种转基因大豆的急性毒性试验使用固定剂量法替代传统霍恩氏法进行研究,认为4种转基因大豆的LD505000mg/kg-BW,属实际无毒级别。各项指标也显示,转基因大豆组未对小鼠生长发育产生不良影响,体重、主要器官及生殖器官质量均未见变化,其中高油酸转基因大豆"EB8011”组的小鼠心脏/体重比与高抗疫霉根腐病转基因大豆"B3W1009"组有显著性差异(p0.05),但判断结果是差异不具有生物学意义。(2)4种转基因大豆的精子畸形试验结果显示,与空白对照组、受体亲本大豆组相比,转基因大豆组精子畸形率无显著性差异,即4种转基因大豆均未对雄鼠生殖细胞产生致突变作用。(3)4种转基因大豆的骨髓细胞微核试验结果显示,与空白对照组、受体亲本大豆组相比,转基因大豆组小鼠的骨髓细胞微核率无显著性差异,即4种转基因大豆均未对小鼠骨髓细胞产生致突变作用。
[Abstract]:Transgenic soybean is one of the earliest transgenic crops introduced and commercially planted in the world and has become the mainstream variety of soybean production in the world. Since the advent of transgenic soybean, it has brought enormous economic and social benefits to the society. At the same time, its safety has also become the focus of discussion, its focus is mainly on whether it will harm human health and destroy the ecological environment, which urgently requires us to speed up the research on the safety evaluation of transgenic soybeans. The edible safety of transgenic soybean was evaluated by toxicological test. In order to determine the potential danger to human body caused by four kinds of transgenic soybean, acute toxicity tests were carried out in male and female mice by gavage, and micronucleus test with chromosome integrity change and chromosome separation change as genetic endpoints was carried out. Sperm abnormality test reflecting potential mutagenicity and reproductive toxicity. The clinical manifestations and histopathological parameters of two kinds of non-transgenic soybean and four kinds of transgenic soybean were compared. The results showed that the acute toxicity test of four transgenic soybeans was carried out by using fixed dose method instead of the traditional Horne method. The LD505000mg / kg-BWs of the four transgenic soybeans were considered to belong to the actual non-toxic grade. The indexes also showed that the transgenic soybean group had no adverse effect on the growth and development of mice, and the body weight, the quality of main organs and reproductive organs did not change. The heart / body weight ratio of the transgenic soybean "EB8011" with high oleic acid was significantly different from that of the transgenic soybean "B3W1009" with high resistance to Phytophthora root rot, but the result was that the difference had no biological significance. The results of the deformity test showed that There was no significant difference in sperm deformity rate in transgenic soybean group compared with blank control group and recipient parent soybean group. The results of micronucleus test of bone marrow cells of four kinds of transgenic soybeans were as follows: compared with blank control group and recipient parent soybean group, four transgenic soybeans had no mutagenic effect on germ cells of male mice. There was no significant difference in micronucleus rate of bone marrow cells in transgenic soybean group, that is, none of the four transgenic soybeans had mutagenicity on bone marrow cells of mice.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S188

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