盐胁迫对忍冬光合作用及金银花品质的影响

发布时间：2018-05-02 21:23

本文选题：忍冬 + 盐胁迫　；参考：《山东中医药大学》2015年硕士论文

【摘要】：目的:分析盐胁迫对红金银花和忍冬光合参数包括净光合速率、气孔导度、胞间二氧化碳浓度、蒸腾速率及金银花品质的影响,进而探讨影响忍冬光合作用的决定性胁迫因子,为盐渍土壤引种忍冬提供一定的理论依据。方法:采用NaCl溶液、中性盐(NaCl:Na2SO4)、碱性盐(NaHCO3:Na2CO3)、混合盐(NaCl、Na2SO4、NaHCO3、Na2CO3)处理红金银花及忍冬扦插苗。用光合仪测定光合参数,采用方差分析、相关性分析及逐步回归分析评价盐胁迫对红金银花和忍冬光合作用的影响,采用高效液相色谱法测定金银花中马钱苷、芦丁、新绿原酸、绿原酸、隐绿原酸、木犀草苷、咖啡酸、异绿原酸B、异绿原酸A和异绿原酸C等10种有效成分含量。结果:①0.1%和0.3%NaCl溶液处理后的红金银花净光合速率均比对照组高,而0.3%NaCl溶液处理下忍冬的净光合速率在处理第四天比对照组低;0.5%NaCl胁迫处理后的金银花中有效成分含量最高。②中性盐胁迫、碱性盐胁迫下,忍冬叶片净光合速率随盐浓度的增加均呈先上升后下降的趋势;在中性盐浓度为15 mmol·L-1,碱性盐浓度为5 mmol·L-1时,忍冬植株光合作用最强,金银花中黄酮类成分的含量随着中性盐胁迫浓度的增加先升高后下降;中性盐和碱性盐胁迫均不利于黄酮类成分的合成;在12.5、25、50 mmol·L-1中性盐胁迫下,环烯醚萜苷成分的含量明显高于对照组,其余各浓度处理组的环烯醚萜苷成分含量均低于对照组;酚酸类成分的含量随着中性盐浓度的增加先下降后上升,而在碱性盐胁迫下随着盐浓度的增加先上升后下降,且各处理组的含量均比对照组低。相关性分析及回归分析结果表明,盐度、缓冲量、pH为三个主要胁迫因子,且盐度是抑制忍冬植株光合作用的主导因素,随着胁迫时间的延长,pH成为影响植株光合作用的次要因素。结论:NaCl胁迫条件下,红金银花的抗盐性高于忍冬,NaCl浓度为0.5%时金银花中有效成分的含量最高;中性盐胁迫和碱性盐胁迫均不利于金银花中黄酮类成分的积累,适宜的盐浓度有利于环烯醚萜苷成分的积累,高浓度中性盐胁迫有利于酚酸类成分的积累,碱性盐胁迫不利于酚酸类成分的积累;混合盐胁迫下,盐度为影响忍冬植株光合作用的决定性因素。
[Abstract]:Objective: to analyze the effects of salt stress on the photosynthetic parameters of Honeysuckle and Lonicera japonica, including net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration, transpiration rate and quality of honeysuckle. It provides some theoretical basis for the introduction of Lonicera japonica in saline soil. Methods: Honeysuckle and Lonicera japonica cuttings were treated with NaCl solution, NaClO _ 3: Na _ 2SO _ 4, NaHCO _ 3: Na _ 2CO _ 3 and NaHCO _ 3H _ 2SO _ 4 + NaHCO _ 3H _ 2CO _ 3. Photosynthetic parameters were measured by photosynthetic apparatus, and the effects of salt stress on photosynthesis of Honeysuckle and Lonicera japonica were evaluated by variance analysis, correlation analysis and stepwise regression analysis. The contents of 10 active components, such as neo- acid, Lv Yuan acid, crypto- acid, luteolin, caffeic acid, isobali_person2# acid B, isob_person2# acid A and iso acid C, etc. Results the net photosynthetic rate of Honeysuckle treated with 10. 1% and 10. 1% 0.3%NaCl solution was higher than that of the control group. However, the net photosynthetic rate of Lonicera japonica in 0.3%NaCl solution was lower than that in control group on the fourth day. The content of active components in honeysuckle treated with 0.3%NaCl solution was the highest under neutral salt stress and alkaline salt stress. The net photosynthetic rate of Lonicera japonica leaves increased first and then decreased with the increase of salt concentration, and the photosynthesis of Lonicera japonica was the strongest when the neutral salt concentration was 15 mmol L -1 and the alkaline salt concentration was 5 mmol L -1. The content of flavonoids in Flos Lonicerae increased first and then decreased with the increase of neutral salt stress concentration, and both neutral salt and alkaline salt stress were not conducive to the synthesis of flavonoids, and under the neutral salt stress of 12.5 ~ 2550 mmol 路L ~ (-1), the content of flavonoids in Flos Lonicerae increased and then decreased with the increase of concentration of neutral salt. The contents of iridoid glycosides were significantly higher than those of the control group, the contents of iridoid glycosides in the other groups were lower than those in the control group, and the contents of phenolic acids decreased first and then increased with the increase of neutral salt concentration. Under alkaline salt stress, the concentration of salt increased first and then decreased, and the content of each treatment group was lower than that of the control group. The results of correlation analysis and regression analysis showed that salinity, buffer amount and pH were the three main stress factors, and salinity was the dominant factor to inhibit photosynthesis of Lonicera japonica. With the extension of stress time, pH was the secondary factor affecting plant photosynthesis. Conclusion the salt resistance of Honeysuckle is higher than that of Lonicera japonicus when the concentration of NaCl is 0.5, while neutral salt stress and alkaline salt stress are not conducive to the accumulation of flavonoids in honeysuckle. The suitable salt concentration is favorable to the accumulation of iridoid glycosides, the high concentration of neutral salt is favorable to the accumulation of phenolic acids, and the alkaline salt stress is not conducive to the accumulation of phenolic acids. Salinity is the decisive factor affecting photosynthesis of Lonicera japonica.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S567.79

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