ZD制剂对马肢蹄软组织损伤作用及对VEGF和bFGF表达的影响

发布时间：2018-05-17 09:42

本文选题：ZD制剂 + 急性软组织损伤马模型　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：ZD制剂是治疗马非开放性软组织损伤的经典验方,为探索其作用机制,本试验建立马前肢急性软组织损伤模型,应用血清学检测,超声方法,红外热成像技术,组织形态学观察,综合评价ZD制剂对马急性软组织损伤的作用效果,采用免疫组织化学方法检测ZD制剂对VEGF、bFGF在肌肉中的表达,研究结果如下:(1)用10匹健康蒙古马随机分成2组,选取左前肢肩部臂部肌肉,用重物砸击,制造急性软组织损伤模型。(2)于造模前、后2h、1d、3d、5 d、7 d、15 d、21 d分别用超声检查评估皮下肿胀、肌肉灰度,用红外热成像仪检测损伤部位局部温度。试验结果表明,早期试验药物组局部温度低于阴性对照组,后期试验药物组的局部温度显著高于阴性对照组,表明ZD制剂对急性软组织损伤早期能够降低局部温度;后期能改善局部温度;ZD制剂对肌肉灰度恢复能力显著优于阳性药物组和阴性对照组,表明ZD制剂对肌肉损伤恢复有良好促进作用;ZD制剂对皮下消肿显著优于阴性对照组;(3)用20匹蒙古马随机分为4组,选取左前肢肩部臂部肌肉,用重物砸击,制造急性软组织损伤模型。于造模前、后12 h、1d、3d、5 d、7 d、15 d、21 d用取样器进行组织取样,制作成石蜡切片,观察其形态学变化。结果表明,试验药物组在损伤后的修复能力优于阳性药物组和阴性对照组。表明ZD制剂能通过促进成纤维细胞、毛细血管新生和肌细胞的生成来进行组织的修复再生。(4)用免疫组织化学方法对阳性细胞分析VEGF、bFGF的表达,结果表明,试验药物组在损伤后VEGF、bFGF的表达优于阴性对照组和阳性药物组,ZD制剂可能通过提高VEGF、bFGF的表达进行损伤的修复。结论:ZD制剂在马急性软组织损伤模型中能够调节受损部位的局部温度,促进皮下组织消肿,调控VEGF、bFGF的表达,促进成纤维细胞、毛细血管,肌细胞再生来进行组织修复。
[Abstract]:ZD preparation is a classical prescription for the treatment of non-open soft tissue injury in horses. In order to explore its mechanism, a model of acute soft tissue injury in horse forelimb was established, and serological examination, ultrasonic method and infrared thermal imaging were used. The effect of ZD preparation on acute soft tissue injury of horse was evaluated synthetically. The expression of VEGF bFGF in muscle was detected by immunohistochemical method. The results were as follows: 1) 10 healthy Mongolian horses were randomly divided into 2 groups. The left forelimb shoulder brachial muscle was selected, and the acute soft tissue injury model was made with heavy object. The model of acute soft tissue injury was made before the model was made, and after 2 h, 1 hour, 1 day, 3 days, 5 days, 15 days and 21 days, respectively, subcutaneous swelling and muscle gray scale were evaluated by ultrasonic examination. The local temperature of the injured area was detected by infrared thermography. The results showed that the local temperature in the early test group was lower than that in the negative control group, and the local temperature in the late trial group was significantly higher than that in the negative control group, indicating that ZD preparation could lower the local temperature in the early stage of acute soft tissue injury. ZD preparation can improve the local temperature significantly better than the positive drug group and the negative control group in the recovery of muscle grayscale. The results showed that ZD had a good promoting effect on the recovery of muscle injury. ZD preparation was significantly better than that of negative control group in treating subcutaneous swelling. 20 Mongolian horses were randomly divided into 4 groups. The left forelimb and shoulder brachial muscles were randomly divided into 4 groups, which were smashed with heavy objects. A model of acute soft tissue injury was established. Before and after 12 h, 1 d, 3 d, 5 d, 7 d, 15 d and 21 d, the tissue was sampled with a sampler to make paraffin sections, and the morphological changes were observed. The results showed that the repair ability of the experimental drug group was better than that of the positive drug group and the negative control group. The results showed that ZD could promote the regeneration of tissue by promoting fibroblast, capillary neogenesis and myocyte formation.) Immunohistochemical method was used to analyze the expression of VEGF bFGF in positive cells. The expression of VEGF bFGF in the experimental group was better than that in the negative control group and the positive drug group, which may be repaired by increasing the expression of VEGF bFGF. Conclusion in the model of acute soft tissue injury of horse, the weight ZD preparation can regulate the local temperature, promote subcutaneous tissue swelling, regulate the expression of VEGF bFGF, and promote the regeneration of fibroblasts, capillaries and myocytes for tissue repair.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.21

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