白音敖包沙地云杉内生真菌多样性及优势菌特性研究

发布时间：2018-06-03 04:52

  本文选题：沙地云杉 + 内生真菌　； 参考：《内蒙古农业大学》2017年硕士论文

【摘要】：本文以内蒙古克什克腾旗白音敖包自然保护区的沙地云杉(Picea mongolica)为研究对象,通过对沙地云杉不同生态型(红果型、紫果型、绿果型)、不同龄级(幼龄、中龄、老龄)和不同部位(枝部、针叶、球果、树皮、根部)内生真菌的分离、纯化和鉴定,确定出优势菌。根据优势菌的生物学特性,判断其适宜的生长环境。同时,通过对优势菌的抑菌性研究,了解内生真菌与病原菌之间的相互关系。结果表明:1.从沙地云杉中共分离出内生真菌890株(其中未产孢37株),经形态显微观察鉴定853株分属于4目5科22属,其中交链孢属和青霉属为优势菌。2.不同龄级的沙地云杉菌株分布不同,虽然菌株数在3个龄级之间相差不大,但是内生真菌的种类随着树龄的增长在逐渐增加。沙地云杉不同部位的内生真菌株数变化顺序排列为枝部树皮部球果针叶根部。3.红果型沙地云杉和紫果型沙地云杉之间的内生真菌种类相似性高。红果型和绿果型沙地云杉之间相似性低。在多样性分布上绿果型沙地云杉中分离得到的内生真菌种群Shannon-Wiener多样性指数和均匀度指数均为最高。红果型沙地云杉获得的内生真菌种群Shannon-Wiener指数和均匀度指数均为最低。4.交链孢菌和青霉菌均在24h的光照下生长更为适宜;交链孢菌最适生长温度为30℃,青霉菌的最适生长温度为25℃;交链孢菌和青霉菌的最适的pH值分别为5.5和6;交链孢菌适宜的氮源和碳源分别是蛋白胨与麦芽糖,青霉菌适宜的氮源和碳源分别是酵母浸膏和蔗糖。5.交链孢菌对丁香褐斑病,杨树褐斑病和杨树烂皮病的抑菌效果较好;而青霉菌对沙枣褐斑病,柳树黑斑病和杨树灰斑病的抑菌效果好。
[Abstract]:In this paper, Picea mongolica (Picea mongolica) from Baiyinaobo Nature Reserve of Keshiketeng Banner in Inner Mongolia was studied. Different ecotypes (red fruit type, purple fruit type, green fruit type, different age class (young, middle age) of Picea mongolicia) were studied. Isolation, purification and identification of endophytic fungi from different parts (branches, needles, cones, bark and roots), and identification of dominant fungi. According to the biological characteristics of dominant bacteria, the suitable growth environment was judged. At the same time, the relationship between endophytic fungi and pathogens was studied by studying the bacteriostasis of dominant bacteria. The results show that: 1. A total of 890 strains of endophytic fungi were isolated from Picea spruce. Among them, 37 strains did not produce spore. By morphological and microscopic observation, 853 strains were identified as belonging to 22 genera, 5 families and 4 orders, among which Alternaria and Penicillium were the dominant bacteria. The distribution of spruce strains in different age classes was different. Although the number of spruce strains was not different among the three age classes, the species of endophytic fungi increased gradually with the increase of tree age. The number of endophytic fungi in different parts of Picea spruce was arranged in the order of branch bark cone coniferous root. The similarity of endophytic fungi between red fruit spruce and purple fruit spruce is high. The similarity between red fruit type and green fruit type spruce is low. The Shannon-Wiener diversity index and evenness index of endophytic fungi isolated from spruce were the highest in diversity distribution. The Shannon-Wiener index and evenness index of endophytic fungi obtained from Picea rubra were the lowest. 4. The optimum growth temperature was 30 鈩，

本文编号：1971538