四川部分地区猪呼吸综合征细菌性病原的调查与分析

发布时间：2018-06-06 00:30

本文选题：猪呼吸道疾病综合征 + 四川省　；参考：《四川农业大学》2015年硕士论文

【摘要】：猪呼吸道疾病综合征(PRDC),是一种由多种病原混合感染而引起发病猪只呼吸系统损伤的疾病,严重危害养猪业的发展。其发病率在35%-80%,死亡率在5%-30%及以上,发病机理复杂,难于防制,是影响当今养猪业的主要问题。引起猪呼吸系统疾病的病原种类繁多,发病日龄覆盖各个阶段,包括哺乳仔猪、保育猪、育肥猪及母猪等,危害面广,给规模化养猪场的防制带来巨大的困难。引起PRDC的细菌性病原包括副猪嗜血杆菌、猪传染性胸膜肺炎放线杆菌、猪多杀性巴氏杆菌、肺炎链球菌等,病毒性病原包括猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪伪狂犬病病毒、猪瘟病毒,猪肺炎支原体也是一种重要致病因素,多种病原之间常以混合感染的方式存在,加强对机体的损伤作用,提高PRDC发病率和死亡率。试验选取了四川省乐山、雅安、新津、天全、宝兴、南充、成都、眉山、内江、遂宁等地区41个规模化养猪厂的53份具有典型呼吸系统疾病症状的样品,对其中的细菌性病原进行了检测,旨在了解四川省部分地区猪呼吸系统疾病的发病情况以及细菌性病原的感染率,为制定科学合理的防制计划提供理论依据。通过对分离菌株统计分析,猪肺炎链球菌的感染率最高,达到69.81%(37/53),多杀巴氏杆菌、副猪嗜血杆菌、传染性胸膜肺炎放线杆菌、李氏杆菌、绿脓杆菌、支气管败血波士杆菌按照感染率从高到低的顺序分别为54.72%(29/53)、47.17%(25/53)、20.75%(11/53)、16.98%(9/53)、13.21%(7/53)、9.43%(5/53)。在病原菌的二重感染病例中,副猪嗜血杆菌与猪肺炎链球菌混合感染率最高,达到32.07%(17/53)；猪肺炎链球菌与多杀巴氏杆菌的混合感染感染率达到30.19%(16/53)；多杀巴氏杆菌与副猪嗜血杆菌的混合感染率达22.64%(12/53)；传染性胸膜肺炎放线杆菌与猪肺炎链球菌混合感染率为13.21%(7/53)；猪肺炎链球菌与李氏杆菌混合感染率为11.32%(6/53)；猪肺炎链球菌与绿脓杆菌混合感染率为9.43%(5/53)；多杀巴氏杆菌与绿脓杆菌混合感染率为7.55%(4/53)；多杀巴氏杆菌与李氏杆菌混合感染率为7.55%(4/53)；副猪嗜血杆菌与绿脓杆菌混合感染率为5.66%(3/53)；副猪嗜血杆菌与李氏杆菌混合感染率为5.66%(3/53)；传染性胸膜肺炎放线杆菌与副猪嗜血杆菌混合感染为5.66%(3/53)；支气管败血波士杆菌与多杀巴氏杆菌感染率为5.66%(3/53)；猪肺炎链球菌与支气管败血波士杆菌混合感染率为5.66%(3/53);绿脓杆菌与李氏杆菌混合感染率为3.77%(2/53);副猪嗜血杆菌与支气管败血波士杆菌混合感染率为1.89%(1/53);支气管败血波士杆菌与李氏杆菌混合感染率为1.89%(1/53)。在病原菌的三重感染病例中,副猪嗜血杆菌、多杀巴氏杆菌及猪肺炎链球菌混合感染率达到11.32%(6/53)；副猪嗜血杆菌、猪肺炎链球菌与绿脓杆菌混合感染率达到5.66%(3/53)；猪肺炎链球菌、传染性胸膜肺炎放线杆菌与多杀巴氏杆菌感染率达到5.66(3/53)；猪肺炎链球菌、绿脓杆菌与多杀巴氏杆菌混合感染率达到3.77%(2/53)；多杀巴氏杆菌、猪肺炎链球菌与李氏杆菌混合感染率达到3.77%(2/53)；多杀巴氏杆菌、猪肺炎链球菌与支气管败血波士杆菌混合感染率达到3.77%(2/53)；副猪嗜血杆菌与、杀巴氏杆菌与绿脓杆菌混合感染率达到1.89%(1/53);副猪嗜血杆菌、支气管败血波士杆菌与李氏杆菌混合感染率达到1.89%(1/53);传染性胸膜肺炎放线杆菌、副猪嗜血杆菌与猪肺炎链球菌混合感染率达到1.89%(1/53)；李氏杆菌、副猪嗜血杆菌与传染性胸膜肺炎放线杆菌混合感染率达到1.89%(1/53)；传染性胸膜肺炎放线杆菌、多杀巴氏杆菌与绿脓杆菌混合感染率达到1.89%(1/53);传染性胸膜肺炎放线杆菌、多杀巴氏杆菌与李氏杆菌混合感染率达到1.89%(1/53)；传染性胸膜肺炎放线杆菌、猪肺炎链球菌与绿脓杆菌混合感染率达到1.89%(1/53)。在病原菌的四重感染病例中,猪肺炎链球菌、副猪嗜血杆菌、绿脓杆菌与多杀巴氏杆菌混合感染率达到1.89%(1/53)；李氏杆菌、副猪嗜血杆菌、猪肺炎链球菌与多杀巴氏杆菌混合感染率达到1.89%(1/53);副猪嗜血杆菌、猪肺炎链球菌、绿脓杆菌与李氏杆菌混合感染率达到1.89%(1/53)；多杀巴氏杆菌、副猪嗜血杆菌、传染性胸膜肺炎放线杆菌与猪肺炎链球菌混合感染率达到1.89%(1/53)：李氏杆菌、多杀巴氏杆菌、绿脓杆菌与猪肺炎链球菌混合感染率达到1.89%(1/53)。
[Abstract]:Porcine respiratory disease syndrome (PRDC) is a disease caused by respiratory system damage caused by mixed infection of various pathogens, which seriously endangering the development of pig industry. Its incidence is in 35%-80%, the mortality rate is 5%-30% and above, the pathogenesis is complex and difficult to prevent. It is the main problem of pig industry. It causes the respiratory disease of pigs. The pathogeny of the disease is wide, and the onset of the disease is covered by various stages, including suckling piglets, pig breeding, fattening pigs and sows, which have a great danger to the prevention and control of large-scale pig farms. The bacterial pathogens of PRDC include Haemophilus accessory pigs, Actinobacillus contagialis, porcine Pasteurella pasteuricus, pneumonia chain. The viral pathogens include porcine reproductive and respiratory syndrome virus, porcine circovirus 2, porcine pseudorabies virus, swine fever virus and Mycoplasma pneumoniae are also an important pathogenic factor. A variety of pathogens often exist in a mixed infection mode, strengthen the damage to the body, increase the incidence of PRDC and mortality. The test selected four In Leshan, Ya'an, Xinjin, Xinjin, Tianquan, Baoxing, Nanchong, Chengdu, Meishan, Neijiang and Suining, 53 samples with typical symptoms of respiratory diseases were tested, and the bacterial pathogens were tested to understand the incidence of respiratory diseases and bacterial pathogens in parts of Sichuan province. The infection rate provides a theoretical basis for the formulation of a scientific and reasonable control plan. By statistical analysis of isolated strains, the infection rate of Streptococcus pneumoniae is the highest, reaching 69.81% (37/53), Pasteurella multocida, Haemophilus vice, contagious Actinobacillus pleuropneumoniae, lelestrus leeniformis, Pseudomonas aeruginosa, and Boss bacilli, in accordance with the infection rate The order of high to low was 54.72% (29/53), 47.17% (25/53), 20.75% (11/53), 16.98% (9/53), 13.21% (7/53), 9.43% (5/53). In the double infection cases of pathogenic bacteria, the mixed infection rate of Haemophilus accessory swine and Streptococcus pneumoniae was the highest, 32.07% (17/53), and the infection rate of mixed infection of Streptococcus pneumoniae and Pasteurella multocida reached 30.. 19% (16/53); the mixed infection rate of Pasteurella multocida and Haemophilus accessory swine was 22.64% (12/53); the mixed infection rate of Actinobacillus pleuropneumoniae and Streptococcus pneumoniae was 13.21% (7/53); the mixed infection rate of Streptococcus pneumoniae and Listeria was 11.32% (6/53), and the rate of mixed infection of Streptococcus pneumoniae and Pseudomonas aeruginosa was 9.43% (6/53). 5/53): the mixed infection rate of Pasteurella multocida and Pseudomonas aeruginosa was 7.55% (4/53); the mixed infection rate of Pasteurella multocida and Listeria was 7.55% (4/53); the mixed infection rate of Haemophilus accessory swine and Pseudomonas aeruginosa was 5.66% (3/53); the mixed infection rate of Haemophilus Haemophilus and Listeria was 5.66% (3/53); the infectious pleuropneumonia caving rod The mixed infection of the bacteria and the Haemophilus accessory swine was 5.66% (3/53); the infection rate of Boss and Pasteurella multiple (3/53) was 5.66% (3/53); the mixed infection rate of Streptococcus pneumoniae and bronchoalveolar septicus was 5.66% (3/53); the mixed infection rate of Pseudomonas aeruginosa and Listeria was 3.77% (2/53), and Haemophilus accessory Haemophilus and bronchial septicus The mixed infection rate of Boss bacilli was 1.89% (1/53), and the mixed infection rate of Boss and Listeria was 1.89% (1/53). In the three cases of pathogen infection, the mixed infection rate of Haemophilus accessory swine, Pasteurella multiple and Streptococcus pneumoniae was 11.32% (6/ 53), Haemophilus accessory swine, Streptococcus pneumoniae and Pseudomonas aeruginosa. The rate of mixed infection reached 5.66% (3/53), the infection rate of Streptococcus pneumoniae, Actinobacillus pleuropneumoniae and Pasteurella multocida reached 5.66 (3/53), and the mixed infection rate of Streptococcus pneumoniae, Pseudomonas aeruginosa and Pasteurella multocida reached 3.77% (2/53), and the mixed infection rate of Streptococcus pneumoniae, Streptococcus pneumoniae and Listeria strains reached 3.7. 7% (2/53); the mixed infection rate of Pasteurella Pasteurella, Streptococcus pneumoniae and Boss bacilli reached 3.77% (2/53); the mixed infection rate of Haemophilus accessory swine and Pasteurella and Pseudomonas aeruginosa reached 1.89% (1/53); the mixed infection rate of Haemophilus accessory swine, Boss and Listeria infection reached 1.89% (1/53); The infection rate of Actinobacillus pleuropneumoniae, Haemophilus accessory swine Haemophilus and Streptococcus pneumoniae reached 1.89% (1/53); the mixed infection rate of Listeria, Haemophilus accessory swine and Actinobacillus pleuropneumoniae was 1.89% (1/53), and the infection rate of contagious Actinobacillus pleuropneumoniae, Pasteurella multiple and Pseudomonas aeruginosa was 1.89% (1). /53); contagious Actinobacillus pleuropneumoniae, the mixed infection rate of Pasteurella multocida and Listeria was 1.89% (1/53); infectious Actinobacillus pleuropneumoniae, Streptococcus pneumoniae and Pseudomonas aeruginosa mixed infection rate reached 1.89% (1/53). In the four infectious diseases of pathogenic bacteria, Streptococcus pneumoniae, Haemophilus vice, Pseudomonas aeruginosa, and Pseudomonas aeruginosa The mixed infection rate of Pasteurella multocida reached 1.89% (1/53); the mixed infection rate of Listeria bacillus, Haemophilus porcine, Streptococcus pneumoniae and Pasteurella Mulus reached 1.89% (1/53); the mixed infection rate of Haemophilus Haemophilus, Streptococcus pneumoniae, Pseudomonas aeruginosa and Listeria was 1.89% (1/53), and Pasteurella multocida, Haemophilus vice and transmission of Bacillus subtilis The mixed infection rate of Actinobacillus pleuropneumoniae and Streptococcus pneumoniae reached 1.89% (1/53): Listeria, Pasteurella multocida, Pseudomonas aeruginosa and Streptococcus pneumoniae reached 1.89% (1/53).
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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