稻瘟病菌中HOPS复合体的亚基MoVps41的功能研究

发布时间：2018-06-06 13:09

  本文选题：稻瘟病菌 + MoVps41　； 参考：《福建农林大学》2017年硕士论文

【摘要】：稻瘟病菌侵染以水稻为主的众多重要农作物,同时也是研究丝状真菌的生长、发育和发病机理的重要模式生物。内吞途径对稻瘟病菌致病性的影响逐渐被证明,在真核生物中,从真菌到植物和动物,HOPS 复合体(Vps11、Vps16、Vps18、Vps33、Vps39 和 Vps41)对同型液泡融合、液泡生成和胞吐运输具有重要作用。然而,它在稻瘟病菌中的功能尚未被研究。本研究搜索到酵母Vps41在稻瘟病菌中的同源蛋白,并对该基因(MGG_03313.7)进行敲除,实验结果证明它是稻瘟病菌液泡分选蛋白41(MoVps41),也是HOPS复合体的一个亚基。MoVps41蛋白的缺失导致稻瘟病菌的有性生殖和无性生殖受阻,生长速率减慢,对水稻叶片、大麦叶片和水稻根部丧失了致病性;MoVps41蛋白缺失导致液泡碎片化,丧失了对胞内过量金属离子的解毒作用;胞外抑制剂测试试验表明MoVps41蛋白的缺失对细胞壁的敏感性、细胞膜的渗透压和囊泡运输造成了严重的影响。
[Abstract]:Magnaporthe grisea infects many important crops, mainly rice, and is also an important model organism to study the growth, development and pathogenesis of filamentous fungi. The effects of endocytosis pathway on pathogenicity of rice blast fungus have been gradually demonstrated. In eukaryotes, the VPS11 / VPS16 / VPS3VPS39 and VPS41 play an important role in the syndromic vacuolar fusion, vacuolar formation and emetic transport from fungi to plants and animals. However, its function in rice blast fungus has not been studied. In this study, the homologous protein of yeast Vps41 in Magnaporthe oryzae was found, and the gene MGG03313.7) was knocked out. The results showed that it was a vacuolar sorting protein of Magnaporthe oryzae, and it was also a subunit of HOPS complex. The deletion of MoVPS41 protein resulted in the inhibition of sexual reproduction and asexual reproduction, and the decrease of growth rate in rice leaves. The loss of pathogenicity of MoVps41 protein in leaves and roots of barley resulted in vacuolar fragmentation and detoxification of intracellular excess metal ions, and the extracellular inhibitor test showed that the deletion of MoVps41 protein was sensitive to cell wall. Membrane osmotic pressure and vesicle transport have serious effects.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S435.111.41
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本文编号：1986639