基于生物絮凝技术的花鳗鲡养殖效果研究
发布时间：2018-07-27 15:04
【摘要】：目前针对生物絮凝的研究主要是环境因素、碳源、对养殖对象的影响等方面,其中C/N在生物絮凝中的影响已经做了大量的研究。不同的碳源和C/N对生物絮凝有着影响差异较大,对生物絮凝养殖系统的稳定性和可控性提出了更高的要求。生物絮凝技术在养殖上的研究对象主要是滤食性的鱼虾类水产动物。花鳗鲡作为一种营养丰富、经济价值高的肉食性鱼类,具有较高的研究价值。针对这个问题,本文提出了将生物絮凝技术应用于花鳗鲡养殖上,研究了成熟的生物絮凝系统零碳源添加对花鳗鲡养殖效果的影响,以及生物絮体替代部分饲料中对花鳗鲡养殖效果的影响。试验内容分为以下三部分:1.生物絮凝养殖模式对花鳗鲡养殖效果的影响试验研究了生物絮凝养殖模式对花鳗鲡生长性能、体组成、消化酶和血液指标等方面的影响,本次试验周期为60天。本次试验设置循环水养殖模式作为对照组、生物絮凝养殖模式作为实验组。每组设置三个平行缸。每缸的养殖密度是10 kg/m3。花鳗鲡初始体重为393.59±11.83 g。实验结果显示,实验组水中总氨氮(TAN)、亚硝氮(NO2--N)和硝氮(NO3--N)含量均显著高于对照组,分别为1.12±0.2 mg/L、0.16±0.05 mg/L和228.44±28.48 mg/L,其中硝态氮在实验期间持续升高。实验结束时实验组和对照组的最终密度(FD)、增重率(WGR)、特定生长率(SCR),饲料系数(FCR)无显著差异(P0.05)。实验组第60天肝脏超氧化酶歧化物(T-SOD)显著高于第30天,较对照组无显著差异。实验组血清碱性磷酸酶(ALP)、谷丙转氨酶(ALT)、甘油三酯(TG)、血糖(GLU)和总蛋白(TP)较对照组无显著差异。实验期间实验组和对照组的胃蛋白酶均降低,其中对照组下降显著。实验组肠蛋白酶活性较对照组无显著差异。2.饲料中添加生物絮体对花鳗鲡养殖效果的影响试验研究了用花鳗鲡粪便培养的生物絮体添加到饲料中,对花鳗鲡生长性能、体组成、消化酶和非特异性免疫方面的影响。本次试验周期为60天。试验分为配合饲料组(对照组)和生物絮体混合饲料组(实验组)。每组设置三个平行缸。每缸的初始密度为12.03±0.23 kg/m3。花鳗鲡初始体重为400.96±6.84 g。试验结果显示,试验结束时,实验组花鳗鲡的体重显著低于对照组(P0.05);试验初期,实验组花鳗鲡增重率为2.19±1.06%,较对照组低(P0.05),试验中后期,实验组增重率与对照组无显著性差异(P0.005);实验组胃蛋白酶较对照组无显著性差异,肠蛋白酶为1723.51±255.35 U/gprot,显著低于对照组;实验组粗蛋白含量显著高于对照组,实验组的粗脂肪、灰分含量较对照组无显著性差异。3.不同C/N条件下生物絮凝水处理效果研究试验研究了不同C/N条件下生物絮凝游离菌群和絮体菌群的水处理效果。试验发现,当C/N为8、12、16时,生物絮凝游离菌群和絮体菌群在12 h内有效去除氨氮,12 h后游离菌群组的氨氮会逐渐升高,絮体菌群组氨氮保持较低含量;当C/N为0、4时,游离菌群组的氨氮最大去除率分别为37.75±7.10%、53.07±2.35%,试验期间,絮体菌的氨氮逐渐降低;
[Abstract]:At present, the research on biological flocculation is mainly environmental factors, carbon sources, and the influence on the breeding objects. The influence of C/N on biological flocculation has been studied much. Different carbon sources and C/N have great influence on biological flocculation, and higher requirements for the stability and controllability of biological flocculation culture system are put forward. The main research object of biological flocculation technology in aquaculture is fish and shrimp aquatic animals with filter feeding. As a kind of carnivorous fish with rich nutrition and high economic value, the flower eel has high research value. In this paper, the biological flocculation technology is applied to the culture of Anguilla japonica, and the mature biological flocculation is studied. The effects of the system zero carbon source on the culture effect of Anguilla anguilla and the effect of biological floc on the culture effect of a part of the eel on the culture of Anguilla japonica. The experimental contents were divided into three parts: 1. the effects of biological flocculation culture model on the culture effect of Anguilla anguilla. The growth performance, body composition and elimination of the biological flocculation and breeding model on the flower eel were studied. The effects of chemical enzyme and blood index were 60 days. This experiment set up the circulation water culture model as the control group and the biological flocculation culture mode as the experimental group. Each group set up three parallel cylinders. The culture density of each cylinder was 10 kg/m3. eel's initial weight of 393.59 + 11.83 g., and the experimental group was in the water. The contents of total ammonia nitrogen (TAN), nitrite (NO2--N) and nitrate (NO3--N) were significantly higher than those of the control group, which were 1.12 + 0.2 mg/L, 0.16 + 0.05 mg/L and 228.44 + 28.48 mg/L respectively. The nitrate nitrogen was increased continuously during the experiment. The final density (FD), weight gain (WGR), specific growth rate (SCR) and feed coefficient (FCR) of the experimental group and the control group were increased at the end of the experiment. The difference (P0.05). The liver Superoxidase dismutase (T-SOD) in the experimental group was significantly higher than that of the control group at sixtieth days. There was no significant difference between the experimental group and the control group. The serum alkaline phosphatase (ALP), the triglyceride (ALT), the triglyceride (TG), the blood glucose (GLU) and the total protein (TP) were not significantly different in the experimental group. The pepsin of the experimental group and the control group was all in the experimental group. There was no significant difference between the control group and the control group. There was no significant difference in the activity of intestinal protease from the control group in the experimental group. The effects of the bio floc on the culture effect of the flower eel were not significantly different from the control group. The biological floc cultured from the feces of the flower eel was added to the feed, and the growth performance, body composition, digestive enzymes and non specific immune aspects of the flower eel were studied in the.2. feed. The test period was 60 days. The test was divided into two groups (the control group) and the biological floc mixed feed group (experimental group). Each group set up three parallel cylinders. The initial weight of each cylinder was 12.03 + 0.23 kg/m3., and the initial weight of Anguilla japonica was 400.96 + 6.84 g. test. The test group was significantly lower than the experimental group. In the control group (P0.05), the weight gain rate of the Anguilla japonica was 2.19 + 1.06% in the experimental group, which was lower than the control group (P0.05). The weight gain rate of the experimental group was not significantly different from the control group (P0.005). The pepsin in the experimental group had no significant difference compared with the control group, and the intestinal protease was 1723.51 + 255.35 U/gprot, significantly lower than the control group; the crude protein in the experimental group was significantly lower than that of the control group. The content of the crude fat and ash content in the experimental group had no significant difference compared with the control group. The effect of biological flocculation water treatment under different C/N conditions was studied under different C/N conditions. The effect of biological flocculation free flora and floc bacteria group under different C/N conditions was studied. The experiment found that when C/N was 8,12,16, biological flocculation of free flora and free bacteria group were found. The ammonia nitrogen in the floc group was effectively removed in 12 h, and the ammonia nitrogen in the free bacteria group increased gradually after 12 h, and the ammonia nitrogen of the floc group kept a low content. When C/N was 0,4, the maximum removal rate of ammonia nitrogen in the free bacteria group was 37.75 + 7.10% and 53.07 + 2.35%, and the ammonia nitrogen of the floc bacteria gradually decreased during the test.
【学位授予单位】：上海海洋大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S965.223
本文编号：2148207
[Abstract]:At present, the research on biological flocculation is mainly environmental factors, carbon sources, and the influence on the breeding objects. The influence of C/N on biological flocculation has been studied much. Different carbon sources and C/N have great influence on biological flocculation, and higher requirements for the stability and controllability of biological flocculation culture system are put forward. The main research object of biological flocculation technology in aquaculture is fish and shrimp aquatic animals with filter feeding. As a kind of carnivorous fish with rich nutrition and high economic value, the flower eel has high research value. In this paper, the biological flocculation technology is applied to the culture of Anguilla japonica, and the mature biological flocculation is studied. The effects of the system zero carbon source on the culture effect of Anguilla anguilla and the effect of biological floc on the culture effect of a part of the eel on the culture of Anguilla japonica. The experimental contents were divided into three parts: 1. the effects of biological flocculation culture model on the culture effect of Anguilla anguilla. The growth performance, body composition and elimination of the biological flocculation and breeding model on the flower eel were studied. The effects of chemical enzyme and blood index were 60 days. This experiment set up the circulation water culture model as the control group and the biological flocculation culture mode as the experimental group. Each group set up three parallel cylinders. The culture density of each cylinder was 10 kg/m3. eel's initial weight of 393.59 + 11.83 g., and the experimental group was in the water. The contents of total ammonia nitrogen (TAN), nitrite (NO2--N) and nitrate (NO3--N) were significantly higher than those of the control group, which were 1.12 + 0.2 mg/L, 0.16 + 0.05 mg/L and 228.44 + 28.48 mg/L respectively. The nitrate nitrogen was increased continuously during the experiment. The final density (FD), weight gain (WGR), specific growth rate (SCR) and feed coefficient (FCR) of the experimental group and the control group were increased at the end of the experiment. The difference (P0.05). The liver Superoxidase dismutase (T-SOD) in the experimental group was significantly higher than that of the control group at sixtieth days. There was no significant difference between the experimental group and the control group. The serum alkaline phosphatase (ALP), the triglyceride (ALT), the triglyceride (TG), the blood glucose (GLU) and the total protein (TP) were not significantly different in the experimental group. The pepsin of the experimental group and the control group was all in the experimental group. There was no significant difference between the control group and the control group. There was no significant difference in the activity of intestinal protease from the control group in the experimental group. The effects of the bio floc on the culture effect of the flower eel were not significantly different from the control group. The biological floc cultured from the feces of the flower eel was added to the feed, and the growth performance, body composition, digestive enzymes and non specific immune aspects of the flower eel were studied in the.2. feed. The test period was 60 days. The test was divided into two groups (the control group) and the biological floc mixed feed group (experimental group). Each group set up three parallel cylinders. The initial weight of each cylinder was 12.03 + 0.23 kg/m3., and the initial weight of Anguilla japonica was 400.96 + 6.84 g. test. The test group was significantly lower than the experimental group. In the control group (P0.05), the weight gain rate of the Anguilla japonica was 2.19 + 1.06% in the experimental group, which was lower than the control group (P0.05). The weight gain rate of the experimental group was not significantly different from the control group (P0.005). The pepsin in the experimental group had no significant difference compared with the control group, and the intestinal protease was 1723.51 + 255.35 U/gprot, significantly lower than the control group; the crude protein in the experimental group was significantly lower than that of the control group. The content of the crude fat and ash content in the experimental group had no significant difference compared with the control group. The effect of biological flocculation water treatment under different C/N conditions was studied under different C/N conditions. The effect of biological flocculation free flora and floc bacteria group under different C/N conditions was studied. The experiment found that when C/N was 8,12,16, biological flocculation of free flora and free bacteria group were found. The ammonia nitrogen in the floc group was effectively removed in 12 h, and the ammonia nitrogen in the free bacteria group increased gradually after 12 h, and the ammonia nitrogen of the floc group kept a low content. When C/N was 0,4, the maximum removal rate of ammonia nitrogen in the free bacteria group was 37.75 + 7.10% and 53.07 + 2.35%, and the ammonia nitrogen of the floc bacteria gradually decreased during the test.
【学位授予单位】：上海海洋大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S965.223
【相似文献】
相关期刊论文 前5条
1 鲁璐;罗国芝;谭洪新;孙大川;;生物絮凝技术处理水产养殖用水效果的初步研究[J];渔业现代化;2010年06期
2 赵宇华;;消化器中的生物絮凝[J];中国沼气;1986年03期
3 唐华钟;罗国芝;谭洪新;鲁璐;梁文艳;;生物絮凝对半咸水养殖水体中固体废弃物的处理效果[J];江苏农业科学;2013年02期
4 张楠;罗国芝;;生物絮凝技术在罗非鱼养殖中的应用[J];渔业现代化;2014年03期
5 张楠;罗国芝;谭洪新;马念念;刘文畅;;生物絮凝技术中碳源的添加策略——以养虾为例[J];水产科技情报;2013年06期
相关博士学位论文 前1条
1 刘绍根;城市污水生物絮凝吸附工艺的特性及模拟研究[D];中国科学技术大学;2010年
相关硕士学位论文 前10条
1 安玉;基于生物絮凝技术的花鳗鲡养殖效果研究[D];上海海洋大学;2017年
2 李文清;不同盐度条件下生物絮凝—罗非鱼养殖效果的研究[D];上海海洋大学;2015年
3 吴盛凯;异位式生物絮凝系统吉富罗非鱼养殖效果研究[D];上海海洋大学;2016年
4 李贵敏;生物絮凝—前置反硝化曝气生物滤池组合工艺处理城市污水效能研究[D];安徽建筑大学;2016年
5 江娟;化学—生物絮凝提高二沉池出水水质实验研究[D];华中科技大学;2011年
6 唐华钟;序批式生物絮凝技术处理水产养殖固体废弃物的初步研究[D];上海海洋大学;2012年
7 刘娟;生物絮凝—再生工艺研究及特性参数的测定[D];安徽建筑工业学院;2010年
8 赵二燕;生物絮凝—再生工艺处理生活污水的数学模拟[D];安徽建筑工业学院;2010年
9 薛二军;洗毛废水生物絮凝中试处理及后继处理可行性的研究[D];天津科技大学;2002年
10 倪丙杰;城市生活污水生物絮凝吸附工艺处理的实验研究[D];合肥工业大学;2005年
，本文编号：2148207
本文链接：https://www.wllwen.com/shoufeilunwen/zaizhiyanjiusheng/2148207.html
