亚麻耐盐突变体的筛选及种质耐盐性的评价
发布时间：2019-01-28 21:06
【摘要】：本研究以甲基磺酸乙酯(EMS)处理亚麻萌动的种子。根据M1代的成株率,在纤用亚麻‘范尼’中筛选出最佳的EMS诱变体系,并筛选出具有耐盐性的突变体以及茎部(株高、茎粗)突变体。并且以来自国内外的97份亚麻种质为研究对象,运用150mmol·L-1NaCl溶液培养,以萌发期种子的发芽率、发芽势、根长、苗长4种生理指标变化为依据,利用隶属函数法评价亚麻萌发期的耐盐性,同时根据不同种质千粒重的变化,分析种子大小与耐盐性的相关性。研究结果如下:1、亚麻突变体库的创建及耐盐突变体的筛选结果表明:(1)0.6%EMS处理萌动亚麻种子,处理时间为4h;在M1代获得叶型、叶色、茎型、茎色和株高变化等26种明显可见性状突变体,其中M2代能稳定遗传的有17种。在该M2群体中,对与纤维产量相关的茎部性状突变体进行了M3代验证,获得M3代茎部突变体种子。(2)以150mmol·L-1 NaCl溶液筛选M2代,获得一株耐盐突变体,其M3代经耐盐性验证获得M4代。2、97份种质耐盐性鉴定结果表明:(1)亚麻耐盐性的评价中,各生理指标权重如下:苗长发芽势根长发芽率;(2)相同欧式距离情况下,分配权重法把97份亚麻种质分为5级,算数平均数法分为4级;在评价作物耐盐性强弱时,加权隶属函数法能更准确的衡量品种的耐盐性;(3)千粒重与亚麻耐盐性(D值)呈正相关,但相关系数较较小;(4)加权隶属函数法把亚麻材料耐盐性分为Ⅰ—Ⅴ级:Ⅰ级-2份材料;Ⅱ级-33份材料;Ⅲ级-16份材料;Ⅳ级-28份材料;Ⅴ级-18份材料。研究补充了前人对国内外亚麻种质耐盐性评价数据库,为今后培育耐盐性较强,农艺性状优异亚麻育种工作提供依据。
[Abstract]:In this study, the germinating seeds of flax were treated with ethyl methanesulfonate (EMS). According to the adult plant rate of M1 generation, the best EMS mutagenesis system was screened from flax 'Fanny', and the salt tolerant mutants and stem (plant height, stem diameter) mutants were screened. 97 flax germplasms from home and abroad were studied and cultured with 150mmol L-1NaCl solution. The changes of germination rate, germination potential, root length and seedling length were taken as the basis. The method of membership function was used to evaluate the salt tolerance of flax during germination, and the correlation between seed size and salt tolerance was analyzed according to the variation of 1000-grain weight of different germplasm. The results were as follows: 1. The establishment of flax mutant bank and the screening of salt tolerant mutants showed that: (1) 0.6%EMS treatment of germinating flax seeds for 4 h; In M1 generation, 26 obvious mutants were obtained, such as leaf type, leaf color, stem type, stem color and plant height change, among which 17 species could be inherited stably in M2 generation. In this M2 population, the mutant of stem traits related to fiber yield was verified by M3 generation, and the seeds of stem mutants of M 3 generation were obtained. (2) A salt tolerant mutant was obtained by screening M2 generation with 150mmol L-1 NaCl solution. The results showed that: (1) in the evaluation of salt tolerance of flax, the weight of each physiological index was as follows: seedling germination rate; (2) under the condition of the same European distance, 97 flax germplasm were divided into five grades by the weight method, the arithmetic average method was divided into 4 grades, and the weighted membership function method could be used to evaluate the salt tolerance of the varieties more accurately when evaluating the salt tolerance of crops. (3) Thousand kernel weight was positively correlated with flax salt tolerance (D value), but the correlation coefficient was relatively small. (4) the salt tolerance of flax was divided into 鈪，
本文编号：2417315
[Abstract]:In this study, the germinating seeds of flax were treated with ethyl methanesulfonate (EMS). According to the adult plant rate of M1 generation, the best EMS mutagenesis system was screened from flax 'Fanny', and the salt tolerant mutants and stem (plant height, stem diameter) mutants were screened. 97 flax germplasms from home and abroad were studied and cultured with 150mmol L-1NaCl solution. The changes of germination rate, germination potential, root length and seedling length were taken as the basis. The method of membership function was used to evaluate the salt tolerance of flax during germination, and the correlation between seed size and salt tolerance was analyzed according to the variation of 1000-grain weight of different germplasm. The results were as follows: 1. The establishment of flax mutant bank and the screening of salt tolerant mutants showed that: (1) 0.6%EMS treatment of germinating flax seeds for 4 h; In M1 generation, 26 obvious mutants were obtained, such as leaf type, leaf color, stem type, stem color and plant height change, among which 17 species could be inherited stably in M2 generation. In this M2 population, the mutant of stem traits related to fiber yield was verified by M3 generation, and the seeds of stem mutants of M 3 generation were obtained. (2) A salt tolerant mutant was obtained by screening M2 generation with 150mmol L-1 NaCl solution. The results showed that: (1) in the evaluation of salt tolerance of flax, the weight of each physiological index was as follows: seedling germination rate; (2) under the condition of the same European distance, 97 flax germplasm were divided into five grades by the weight method, the arithmetic average method was divided into 4 grades, and the weighted membership function method could be used to evaluate the salt tolerance of the varieties more accurately when evaluating the salt tolerance of crops. (3) Thousand kernel weight was positively correlated with flax salt tolerance (D value), but the correlation coefficient was relatively small. (4) the salt tolerance of flax was divided into 鈪，
本文编号：2417315
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