边缘叶癫痫小鼠模型中癫痫危险程度与海马区尿酸水平的相关性

发布时间：2018-01-04 16:39

本文关键词：边缘叶癫痫小鼠模型中癫痫危险程度与海马区尿酸水平的相关性　出处：《实用医学杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的通过建立边缘叶癫痫小鼠模型,并通过基因及药物手段改变小鼠体内尿酸的水平,从而探讨小鼠海马区尿酸水平与其癫痫发病的关系。方法实验材料为正常雄性小鼠C57BL/6和变异C57BL/6小鼠,变异小鼠包括尿酸酶基因敲入(UOx-OE)以及基因敲除(UOx-KO)两种类型;实验分组主要包括正常C57BL/6小鼠(CTR)、海人酸(KA)处理小鼠(KA组)、KA结合别嘌呤醇处理小鼠(All组)、KA处理UOx-KO小鼠(KA+KO组)及KA处理UOx-OE小鼠(KA+OE组);实验时,记录各组小鼠的活动情况、达到癫痫时经历的时间、癫痫持续时间;采用微透析技术收集小鼠脑部海马区的透析液,并运用高压液相色谱技术检测不同处理情况下透析液中尿酸的含量;比较不同分组之间癫痫小鼠的海马区尿酸水平、癫痫情况、癫痫潜伏期以及持续时间的差异。结果分组包括正常及变异的C57BL/6小鼠各24只,至实验结束,仅All组处理小鼠死亡1只;经KA处理后,小鼠出现癫痫症状;与KA组相比,KA结合别嘌呤醇处理后,小鼠海马区透析液中尿酸水平下降,小鼠全身性癫痫次数减少,差异有统计学意义(P0.05);与KA组相比,KA处理UOx-KO小鼠后,小鼠海马区透析液尿酸水平、癫痫潜伏期以及小鼠全身性癫痫次数出现上升,差异有统计学意义(P0.05);与KA组相比,KA处理UOx-OE小鼠后,癫痫潜伏期、持续时间、小鼠部分以及全身性癫痫次数无明显变化(P0.05)。结论海马区尿酸水平的高低可能影响小鼠癫痫的病情,提示尿酸及其信号通路可作为治疗癫痫的潜在靶标。
[Abstract]:Objective to establish a mouse model of limbic seizure, and by means of changing the gene and drug uric acid in mice, so as to explore the relationship of uric acid and mice hippocampus epilepsy. Methods the experimental material for normal male mice C57BL/6 and mutation C57BL/6 mice, including the variation of uricase gene knock in mice (UOx-OE) and knockout (UOx-KO) two types; experimental groups including normal C57BL/6 mice (CTR), kainic acid (KA) treatment in mice (KA group), KA combined with allopurinol treated mice (All group), KA treatment of UOx-KO mice (KA+KO group) and KA treated UOx-OE mice (KA+OE group); experiment. Records of the mice, to experience the time of epilepsy, epilepsy duration; dialysate using microdialysis to collect mouse hippocampus, and the use of high pressure liquid dialysis liquid chromatography for detection of different processing conditions in urine The content of acid in hippocampus; uric acid level between different groups were compared in epileptic mice epilepsy, seizure latency and duration differences. Results including normal C57BL/6 mice and the variation of each 24, to the end of the experiment, only group All treated mice died 1; after KA treatment, mice showed symptoms of epilepsy; comparison with the KA group, KA combined with allopurinol treatment, uric acid levels in mice hippocampus dialysate decreased in mice, generalized epilepsy decreased, the difference was statistically significant (P0.05); compared with KA group, KA treated UOx-KO mice after liquid uric acid levels in hippocampus of epileptic mice and mice incubation period of dialysis, the number of generalized epilepsy rose, the difference was statistically significant (P0.05); compared with KA group, KA treated UOx-OE mice after seizure latency, duration, and number of generalized epilepsy mice did not change significantly (P0.05). Conclusion the hippocampus The level of uric acid may affect the state of epilepsy in mice, suggesting that uric acid and its signaling pathway can be used as a potential target for the treatment of epilepsy.

【作者单位】：河北省沧州市中心医院神经内三科;河北省沧州市中心医院老年病房;河北省沧州市医学高等专科学校;
【基金】：沧州市科技计划项目(编号:151302138)
【分类号】：R-332;R742.1
【正文快照】： 癫痫是当今世界范围内影响人们身体健康的一大神经系统疾病,其疾病特征包括自发及反复发作的痉挛现象[1],相关研究发现疾病的发生主要归因于遗传或社会因素引起的持续上升的大脑兴奋性[2],当代社会癫痫病情不仅会对自身造成极大的危害,对家庭以及社会同样造成巨大的负担。危险

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