血清不耐热物质对噬菌体PL39杀灭铜绿假单胞菌的影响

发布时间：2018-01-04 18:37

  本文关键词：血清不耐热物质对噬菌体PL39杀灭铜绿假单胞菌的影响　出处：《中国病原生物学杂志》2017年07期 　论文类型：期刊论文

  更多相关文章： 铜绿假单胞菌 噬菌体 人血清 小鼠血清 不耐热物质

【摘要】：目的观察人血清和鼠血清中不耐热物质对噬菌体PL39杀灭不同克隆铜绿假单胞菌的影响。方法采用双层平板法筛选PL39敏感宿主菌;根据ERIC-PCR图谱分析敏感宿主菌的遗传差异性。分别添加新鲜及热灭活人血清和鼠血清,观察其对PL39杀灭不同敏感宿的影响。结果筛选到9株PL39敏感宿主菌,其中2株菌ERIC指纹图谱无明显不同,其他菌株的指纹图谱明显不同。9株菌均能完全抵抗人血清和鼠血清的溶菌作用;噬菌体PL39在两种血清中均能保持恒定的成斑活性,但在人血清中对9株宿主菌的杀灭作用均被抑制;在小鼠血清中,PL39对L39菌有一定杀灭作用,对其他宿主菌无杀灭作用;两种血清热灭活(56℃,30min)后对PL39杀灭不同宿主菌作用无显著影响,与营养肉汤中的PL39杀菌作用相比差异无统计学意义(P0.05)。结论人血清和鼠血清中的不耐热物质均可影响噬菌体PL39对其宿主菌的杀灭作用。
[Abstract]:Objective to observe the effect of heat-resistant substances in human serum and mouse serum on bacteriophage PL39 killing Pseudomonas aeruginosa clones from different clones. The genetic diversity of sensitive host bacteria was analyzed according to ERIC-PCR map. Fresh and heat-inactivated human serum and mouse serum were added respectively. The results showed that 9 strains of PL39 sensitive host bacteria were screened, of which 2 strains had no significant difference in ERIC fingerprints. The fingerprinting of other strains was obviously different. 9 strains could completely resist the bacteriolytic effect of human serum and mouse serum. Bacteriophage PL39 could maintain constant plaque formation activity in both sera, but its killing activity against 9 host strains in human serum was inhibited. In the serum of mice, PL39 had a certain killing effect on L39 bacteria, but no killing effect on other host bacteria. There was no significant effect on the killing of different host bacteria by PL39 after two kinds of serum heat inactivation (56 鈩，

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