抗坏血酸促进骨髓间充质干细胞的上皮转分化

发布时间：2018-01-05 04:19

本文关键词：抗坏血酸促进骨髓间充质干细胞的上皮转分化　出处：《中国组织工程研究》2017年01期 　论文类型：期刊论文

【摘要】：背景:研究表明,抗坏血酸具有增强血管弹性、强化骨骼、抗氧化的作用。目的:通过体外细胞分子生物学实验,观察抗坏血酸对骨髓间充质干细胞上皮转分化的促进作用。方法:不同浓度(0,10,20,40μmol/L)抗坏血酸处理骨髓间充质干细胞,光镜下观察骨髓间充质干细胞形态变化,筛选出最适处理浓度和时间(40μmol/L抗坏血酸干预48 h),进一步Realtime PCR检测骨髓间充质干细胞上皮相关分子E-钙黏蛋白、N-钙黏蛋白及间质相关分子snail1、转化生长因子β的表达,Western blot检测骨髓间充质干细胞内干性相关分子Oct4,Nanog,Sox2的表达。结果与结论:(1)光镜下观察,抗坏血酸处理24 h,骨髓间充质干细胞的形态开始由长梭形逐渐向形态较圆润的上皮细胞转化。随着抗坏血酸浓度的上调,形态学变化越显著。抗坏血酸处理48 h,光镜下约有90%以上的细胞出现以上变化;(2)抗坏血酸可通过上调骨髓间充质干细胞上皮相关分子(N-钙黏蛋白,E-钙黏蛋白)并下调间质相关分子(snail1,转化生长因子β)的表达,促进骨髓间充质干细胞间质上皮转化;(3)抗坏血酸在促进骨髓间充质干细胞间质上皮转化的同时,骨髓间充质干细胞干性(Oct4,Sox2,Nanog表达)未发生明显变化。
[Abstract]:Background: studies have shown that ascorbic acid can enhance vascular elasticity, enhance bone, antioxidant effects. Objective: through in vitro cell molecular biological experiments. To observe the promoting effect of ascorbic acid on the epithelial transdifferentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: BMSCs were treated with ascorbic acid at different concentrations. The morphologic changes of bone marrow mesenchymal stem cells were observed under light microscope. The optimal concentration and time of treatment were 40 渭 mol/L and 48 h after ascorbic acid treatment. Furthermore, Realtime PCR was used to detect the expression of E-cadherin and snail1, and transforming growth factor 尾 (TGF- 尾) in bone marrow mesenchymal stem cells. Western blot was used to detect the expression of dry related molecule Oct4GN Nanog-Sox2 in bone marrow mesenchymal stem cells. After treatment with ascorbic acid for 24 h, the morphology of bone marrow mesenchymal stem cells began to transform from long fusiform to round epithelial cells, with the increase of ascorbic acid concentration. The more obvious the morphological changes were, the more than 90% cells appeared above changes under light microscope after 48 h treatment with ascorbic acid. (2) ascorbic acid can up-regulate the expression of mesenchymal stem cell epithelial-associated molecule (N- cadherin) and down-regulate the expression of mesenchymal related molecule (TGF- 尾). Promote mesenchymal stem cell mesenchymal epithelium transformation; While ascorbic acid promoted mesenchymal stem cell mesenchymal epithelium transformation, the expression of Oct4 and Sox2 Nanog in bone marrow mesenchymal stem cells did not change significantly.
【作者单位】：新乡医学院第一附属医院血液二科;
【基金】：河南省医学科技攻关计划项目(201602152)~~
【分类号】：R329.2
【正文快照】： 文章快速阅读:抗坏血酸对骨髓间充质干细胞上皮转分化的促进作用不同浓度抗坏血酸处理骨髓间人骨髓间实时定量PCR实Wesern blot检测充质干细胞,筛选出最适处理充质干细验检测间充质及上Oct4,Sox2,Nanog浓度和时间胞培养(40μmol/L抗坏血皮相关分子表达蛋白表达酸干预48 h)文题

【相似文献】