慢性阻塞性肺疾病大鼠模型气道弹性及炎症因子改变
本文关键词: 肺疾病 慢性阻塞性 胶原 基质金属蛋白酶 金属蛋白酶抑制物 热休克蛋白 出处:《中华临床医师杂志(电子版)》2016年22期 论文类型:期刊论文
【摘要】:目的探讨慢性阻塞性肺疾病(COPD)大鼠模型中气道弹性及炎症因子的改变。方法将40只Wistar大鼠随机分为5组(G1~G5),每组再分两组(COPD模型组及正常对照组),采用烟熏加气管内注入脂多糖的方法制造COPD大鼠模型。用HE染色及马松(Masson)三色染色评价大鼠气道壁病理变化和胶原纤维的变化;采用ELISA法检测血清中基质金属蛋白酶9(MMP9)、金属蛋白酶抑制物1(TIMP1)及热休克蛋白27(HSP27)的表达水平。COPD模型组血清MMP9、TIMP1及HSP27表达水平的各组间比较采用方差分析,组间两两比较采用LSD-t检验,P0.05为有统计学差异。结果 G1、G4、G5组中COPD模型组血清MMP9和TIMP1的表达水平高于正常对照组的表达水平,各COPD模型组血清HSP27的表达水平均高于正常对照组;COPD模型组MMP9的表达总体呈上升趋势,差异有统计学意义(F=17.491,P0.05);COPD模型组TIMP1的表达总体呈上升趋势,G3与G5组(P=0.000 0470.001)及G4与G5组(P=0.000 0280.001)差异有统计学意义;COPD模型组MMP9/TIMP1的比值随MMP9和TIMP1改变而变化,差异有统计学意义(F=15.076,P0.05);COPD模型组HSP27的表达呈轻微上升趋势,G4与G5组之间差异有统计学意义(P=0.0270.05)。结论 MMP9/TIMP1的平衡及胶原纤维、HSP27的改变可以作为评价COPD进展的重要指标。
[Abstract]:Objective to investigate the changes of airway elasticity and inflammatory factors in rats with chronic obstructive pulmonary disease (Wistar). Methods 40 Wistar rats were randomly divided into 5 groups. Each group was divided into two groups: COPD model group and normal control group. The rat model of COPD was established by fumigation and intratracheal injection of lipopolysaccharide. The pathological changes of airway wall and the changes of collagen fibers were evaluated by HE staining and Ma Song Masson staining. Serum matrix metalloproteinase 9 (MMP9) was determined by ELISA. The expression level of metalloproteinase inhibitor 1 (TIMP 1) and heat shock protein 27 (HSP27). The expression level of TIMP1 and HSP27 were analyzed by ANOVA, and LSD-t test was used to compare the expression levels of G1G4 and G1G4. The expression of serum MMP9 and TIMP1 in the COPD model group was higher than that in the normal control group, and the expression level of serum HSP27 in each COPD model group was higher than that in the normal control group. The expression of MMP9 in COPD model group showed an upward trend, and the difference was statistically significant (P 0.05). The expression of TIMP1 in COPD model group showed a general upward trend. There was significant difference between G3 and G5 group (0.000 0470.001) and G4 and G5 group (0.000 0280.001). In COPD model group, the ratio of MMP9/TIMP1 changed with the changes of MMP9 and TIMP1, and the difference was statistically significant (P 0.05). The expression of HSP27 in COPD model group showed a slight upward trend. The difference between G4 and G5 groups was statistically significant (P < 0.05). Conclusion the balance of MMP9/TIMP1 and collagen fibers are significant. The change of HSP27 can be used as an important index to evaluate the progress of COPD.
【作者单位】: 扬州大学第二临床学院影像科;天津市人民医院影像中心;东南大学附属中大医院放射科;
【基金】:扬州市社会发展科技攻关计划立项课题(2012136)
【分类号】:R563.9;R-332
【正文快照】: 慢性阻塞性肺疾病(chronic obstructive pulmonarydisease,COPD)是一种高患病率、高死亡率的呼吸系统疾病,目前位居全球死因的第4位。在未来几十年内,COPD仍是一项严重的公共卫生问题,预计到2020年COPD将是全球第5位负担最重的疾病及第3位致死性疾病。我国COPD患病人群中每年
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