脾阳虚加湿邪大鼠内外合邪模型建立的研究

发布时间：2018-01-20 05:18

本文关键词： 脾阳虚内外合邪潮湿环境一般情况　出处：《中华中医药杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的:探讨潮湿环境因素对正常及脾阳虚大鼠一般情况的影响,为建立内外合邪动物模型做初步尝试。方法:选取40只SD大鼠随机分为对照组、脾阳虚组、外湿组、脾阳虚加湿组,每组10只。脾阳虚组模型制作采用番泻叶灌胃,皮下注射利血平与强迫大鼠游泳交替进行的复合方法。造模后,给予外湿组与脾阳虚加湿组潮湿环境1周,于造模前后观察并记录各组大鼠的体质量、进食量、饮水量、精神活动、皮毛光泽度及大便性状并进行宏观症候评分。结果:造模后,脾阳虚加湿组大鼠体质量显著减轻,较其余3组显著降低(P0.01,P0.05)。脾阳虚加湿组大鼠在进食、饮水、精神状态、毛色、大便性状等方面较其余3组发生了一定的变化。宏观症候评分较脾阳虚组显著降低(P0.01)。结论:潮湿环境因素对正常及脾阳虚大鼠的一般情况都有影响,对脾阳虚大鼠影响更为明显。
[Abstract]:Objective: to explore the effect of wet environment on the general situation of normal and spleen yang deficiency rats and to make a preliminary attempt to establish the animal model of combination of internal and external pathogenic factors. Methods: 40 SD rats were randomly divided into control group and spleen yang deficiency group. The model of spleen yang deficiency group was made by perfusing Senna leaf, subcutaneously injected reserpine and forced swimming alternately. The wet environment of the external dampness group and the spleen yang deficiency humidification group was given for 1 week. The body weight, food intake, drinking water and mental activity of the rats were observed and recorded before and after the model making. Results: after modeling, the body mass of rats in spleen yang deficiency and humidification group significantly decreased, compared with the other three groups significantly lower than the other three groups (P0.01). P0.050.The spleen yang deficiency humidification group rats were eating, drinking water, mental state, fur color. Compared with the other three groups, the stool traits changed to some extent. The macroscopic symptom score was significantly lower than that of the spleen yang deficiency group (P0.01). Conclusion: the wet environment factors have influence on the general condition of normal and spleen yang deficiency rats. The effect on spleen yang deficiency rats was more obvious.
【作者单位】：湖北中医药大学;山西中医学院;太原市中心医院北院;
【基金】：国家自然科学基金资助项目(No.81173155)~~
【分类号】：R-332;R259
【正文快照】： 中医学认为,湿为重浊有质之邪,属阴,其性黏滞、趋下,不易速去;同时易阻塞气机,损伤阳气。《素问·太阴阳明论》“阳受风气,阴受湿气”。不同性质的邪气,对人体不同部位的侵犯有一种易感趋向。湿为阴邪,故人体阴分受之,邪气袭人,同类相聚,湿为阴邪而易伤下、伤阴、伤脾。《素问

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