雷帕霉素对未成年癫痫模型大鼠的毒性作用

发布时间：2018-01-22 18:29

本文关键词： 雷帕霉素癫痫抗惊厥药　出处：《中国神经精神疾病杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的利用戊四氮(pentylenetetrazol,PTZ)慢性点燃Sprague Dawley大鼠(SD大鼠)模型观察在点燃初期腹腔注射雷帕霉素(rapamycin,RAPA)能否抑制癫痫发生以及雷帕霉素在治疗中的安全性。方法将6~8周龄SD雄性大鼠随机分为雷帕霉素干预组PTZ+RAPA及其对照组PTZ+NS(normal saline)及NS组,观察1周(w)、2周(w)、4周(w)、6周(w)共4个时间点,每亚组12只,观察实验动物体质量的变化、死亡率及癫痫发作情况。结果各组实验大鼠的死亡率:PTZ+RAPA组为22.9%,PTZ+NS组为10.4%,NS组为0%。PTZ+RAPA组与PTZ+NS组各个相对应的时间点(1 w,2 w,4 w,6 w)体质量差均有统计学差异(P0.001)。6周时PTZ+RAPA组的点燃率为66.7%,PTZ+NS组的点燃率为58.3%,二者无统计学差异(P0.05)。PTZ+RAPA组与PTZ+NS组相对应时间点(1 w,2 w,4 w,6 w)发作分值的两两比较差异无统计学意义(P0.05)。结论雷帕霉素未能减少或抑制未成年大鼠的癫痫发作,但能明显降低未成年SD大鼠的体质量,可能有一定的毒副作用。
[Abstract]:Objective to use pentylenetetrazol in pentylenetetrazol. The model of Sprague Dawley rats with chronic kindling was observed and rapamycin was injected intraperitoneally at the early stage of kindling. Rapa). Methods male Sprague-Dawley rats aged 68 weeks were randomly divided into PTZ RAPA group and PTZ NSN control group. Normal saline) and NS group. A total of 4 time points (12 rats per subgroup) were observed for 1 week, 2 weeks, 4 weeks, and 6 weeks, respectively. The changes of the mass of experimental animals were observed. Results the death rate of rats in the RAPA group was 22. 9% and 10.4% in the NS group. In NS group, the corresponding time points of 0% PTZ RAPA group and PTZ NS group were 1, 2 and 4 weeks. There was significant difference in body mass difference at 6 wk. The ignition rate of PTZ RAPA group was 66.7 and that of PTZNS group was 58.3%. There was no statistical difference between the two groups (P 0.05). PTZ RAPA group and PTZ NS group had a corresponding time point of 1 week or 2 weeks or 4 weeks. There was no significant difference in seizure score between two groups (P0.050.Conclusion Rapamycin can not reduce or inhibit the seizure of juvenile rats. But it can obviously reduce the body weight of SD rats, which may have some side effects.
【作者单位】：首都医科大学良乡教学医院神经内科;解放军总医院神经内科;
【基金】：国家自然科学基金项目(编号:81271438)
【分类号】：R-332;R742.1
【正文快照】： *首都医科大学良乡教学医院神经内科(北京102400)△解放军总医院神经内科癫痫患者发作时间的不确定性和大部分需要长期药物治疗给患者本人及家属带了沉重的心理和经济负担。目前癫痫的治疗主要还是靠抗癫痫药物,少数患者可以行根治或姑息性手术治疗、迷走神经电刺激、磁刺激等

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