高原低氧致应激性溃疡大鼠模型的建立

发布时间：2018-01-28 12:40

本文关键词： 应激性溃疡高原低氧模型　出处：《重庆医学》2017年27期 　论文类型：期刊论文

【摘要】：目的建立大鼠高原低氧致应激性溃疡实验动物模型。方法将大鼠分为6组:常氧组、高原低氧2d组、高原低氧4d组、高原低氧6d组、高原低氧8d组和高原低氧10d组。除常氧组外,其余各组大鼠分别在低压氧舱内(模拟海拔5 000m)饲养2、4、6、8d和10d。观察各组大鼠胃黏膜溃疡指数、胃液量、胃蛋白酶活性和胃液pH值的变化。结果大鼠在低压氧舱内模拟海拔5 000m高原低氧2、4、6、8、10d后,均可见胃黏膜多个不同程度的点状和线状出血、糜烂及溃疡形成;溃疡指数明显增加;胃液量和pH值均明显下降,胃蛋白酶活性明显升高。各指标变化均在高原低氧第6天时最明显。结论大鼠在模拟海拔5 000m的低压氧舱内饲养2~10d,均可建立高原低氧致应激性溃疡动物模型,且最佳时间是6d。
[Abstract]:Objective to establish an experimental model of stress ulcer induced by high altitude hypoxia in rats. Methods Rats were divided into 6 groups: normoxic group, high altitude hypoxia group for 2 days, high altitude hypoxia group for 4 days and altitude hypoxia group for 6 days. The rats in the high altitude hypoxia group (8 d) and the high altitude hypoxia group (10 d group) were fed with 2 chrysanthemum 6 in the hypobaric oxygen chamber (simulated altitude 5 000 m) except the normoxic group. The gastric mucosal ulcer index, gastric juice volume, pepsin activity and gastric pH were observed at 8 and 10 days. Results the rats were exposed to hypoxia at altitude of 5 000 m in the hypobaric oxygen chamber. 10 days later, gastric mucosal bleeding, erosion and ulceration were observed. Ulcer index increased significantly. Gastric juice volume and pH decreased significantly. The activity of pepsin was significantly increased, and the changes of each index were most obvious at the 6th day after hypoxia at high altitude. Conclusion the rats were fed in a hypobaric oxygen chamber at a simulated altitude of 5 000 m for 2 min for 10 days. The animal model of stress ulcer induced by hypoxia at high altitude was established, and the best time was 6 days.
【作者单位】：青海省西宁市第一人民医院;青海大学医学院;
【基金】：国家自然科学基金资助项目(81360687)
【分类号】：R-332;R573.1
【正文快照】： 应激性溃疡是机体在各种应激因素(创伤、手术、低氧、寒冷、心理因素等)的刺激下发生的胃肠道黏膜急性糜烂、溃疡、出血等,病情危重,病死率高[1]。高原是人类最具挑战性的环境之一,高原环境具有氧分压低、紫外线强、气候寒冷、辐射高、气候多变等特点。高原低氧环境可引起机体

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