兔心肌缺血再灌注损伤模型制备的心肌声学造影研究

发布时间：2018-01-31 17:02

本文关键词： 兔心肌缺血再灌注损伤心肌声学造影　出处：《中国超声医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨简单有效的兔心肌缺血再灌注损伤模型制备方法,并利用心肌声学造影评价兔心肌缺血再灌注损伤。方法将70只日本大耳白兔随机分为2组:35只心肌缺血/再灌注组(I/R)和35只假手术组(SH)。I/R组阻断冠状动脉左前降支90min,再灌注60、120、180min及1周;SH组开胸后同一部位只穿线不阻断。观察两组兔术前及术后各时段心肌声学造影的变化,最后行HE及MASSON染色。结果成功制备兔心肌缺血再灌注损伤模型30只,I/R组缺血90min至再灌注180min兔损伤节段造影剂视频强度超声均值对比术前及SH组均有不同程度减低,且差异有统计学意义(P0.05)。I/R组兔术后HE及MASSON染色均证实有心肌损伤病理表现,随着早期再灌注时间的延长损伤程度逐渐加重。结论通过阻断兔冠状动脉左前降支90min后解除阻断使其再灌注可成功建立心肌缺血再灌注损伤模型,心肌声学造影可以有效评价心肌缺血再灌注损伤。
[Abstract]:Objective to investigate a simple and effective method for the preparation of rabbit myocardial ischemia-reperfusion injury model. Myocardial contrast echocardiography was used to evaluate myocardial ischemia-reperfusion injury in rabbits. Methods 70 Japanese white rabbits were randomly divided into 2 groups: I / R group (n = 35) and sham operation group (n = 35). The left anterior descending coronary artery was blocked for 90 minutes in the I / R group. The reperfusion time was 60,120,180min and 1 week. The changes of myocardial contrast echocardiography were observed before and after operation in the SH group. Finally, HE and MASSON staining were performed. Results 30 rabbit models of myocardial ischemia-reperfusion injury were successfully established. Compared with preoperative and SH groups, the mean value of video intensity of segmental contrast medium in I / R group was significantly lower than that in group I / R for 90 min to 180 min after reperfusion. The difference was statistically significant (P 0.05). The pathological features of myocardial injury were confirmed by HE and MASSON staining in group I / R after operation. Conclusion the model of myocardial ischemia-reperfusion injury can be successfully established by blocking the left anterior descending branch of coronary artery 90 minutes later and unblocking it. Myocardial contrast echocardiography can effectively evaluate myocardial ischemia reperfusion injury.
【作者单位】：昆明医科大学第四附属医院云南省第二人民医院超声科;
【基金】：国家自然科学基金项目(No.81460062);国家自然科学基金项目(No.81660084) 云南省科技厅-昆明医科大学应用基础研究联合专项(No.2013FB108)
【分类号】：R445.1;R542.2;R-332
【正文快照】： 再灌注治疗可以挽救濒临坏死的心肌,改善预后,降低病死率[1],但再灌注治疗过程同时存在再灌注损伤。建立有效的心肌缺血再灌注损伤(myocardial ischemia reperfusion injury,MIRI)动物模型是研究MIRI的重要前提。心肌声学造影(myocardial contrast echocardiography,MCE)是近

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