腹腔高压作用时间对大鼠肝肾功能的影响

发布时间：2018-02-03 10:05

  本文关键词： 腹腔间隔室综合征(ACS) 腹腔内高压(IAH) 肝肾功能 动物模型　出处：《广东医学》2017年S1期 　论文类型：期刊论文

【摘要】：目的建立SD大鼠腹腔高压(IAH)/腹腔间隔室综合征(ACS)动物模型,研究维持20 mm Hg腹腔内压(IAP)时间长短与SD大鼠肝肾功能损伤的关联性。方法将96只SD大鼠按随机数字表法,随机等量分为实验组(n=48)和对照组(n=48)。实验组腹腔内注入氮气,维持IAP为20 mm Hg水平,建立IAH/ACS模型,并于1/2、1、2、3、4、5 h测定两组动脉血中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肌酐(Cr)和尿素氮(BUN)的含量。结果 IAP为20 mm Hg时,达到IAH状态,维持时间3 h,SD大鼠肝肾功能未出现显著异常。当维持IAH 3 h、5 h时,实验组BUN值显著高于对照组,两组比较差异有统计学意义(P0.05);维持IAH 4 h时,实验组Cr值显著高于对照组,两组差异有统计学意义(P0.05);当维持IAH 5 h时,实验组ALT及AST值显著高于对照组,两组比较差异有统计学意义(P0.05)。结论在恒定的IAH条件下,随着维持时间延长,对SD大鼠肝肾功能的损害逐渐增大。
[Abstract]:Objective to establish an animal model of IAHV / ACSs in SD rats with intraperitoneal hypertension. To study the relationship between the duration of maintaining 20 mm Hg intraperitoneal pressure (IAPs) and the damage of liver and kidney function in SD rats. Methods 96 SD rats were randomly divided into two groups. The experimental group was randomly divided into two groups: the experimental group (n = 48) and the control group (n = 48). Nitrogen was injected into the abdominal cavity of the experimental group and the IAP was maintained at the level of 20 mm Hg. The IAH/ACS model was established. The alanine aminotransferase (alt) and aspartate aminotransferase (AST) in arterial blood were measured at 1 / 2, 1 / 2, 2, 2, 3, 4 and 5 h. Results when IAP was 20 mm Hg, the content of Cr (Cr) and bun (bun) reached the state of IAH and maintained for 3 h. There was no significant abnormality in liver and kidney function in SD rats. When IAH was maintained for 3 h or 5 h, the BUN value of the experimental group was significantly higher than that of the control group, and the difference between the two groups was statistically significant (P 0.05). When IAH was maintained for 4 h, Cr in the experimental group was significantly higher than that in the control group, and the difference between the two groups was statistically significant (P 0.05). When IAH was maintained for 5 h, the ALT and AST values of the experimental group were significantly higher than those of the control group, and the difference between the two groups was statistically significant (P 0.05). With the prolongation of maintenance time, the damage to liver and kidney function of SD rats increased gradually.
【作者单位】： 昆明医科大学第一附属医院胃肠外科;云南省消化病研究所;
【基金】：云南省科学技术厅-云南省科技计划项目(编号:2014FB021) 云南省卫生计生委-云南省卫生科技计划项目(编号:2014NS124,2016NS003)
【分类号】：R-332;R656.1
【正文快照】： 2006年世界腹腔间隔室综合征协会(World So-ciety for Abdominal Compartment Syndrome,WSACS)对腹腔高压(IAH)/腹腔间隔室综合征(ACS)作出了较为规范的定义[1]:成年危重病患者腹腔内压(IAP)在4~7 mm Hg范围内,IAP持续≥12 mm Hg或反复出现病理学表现则为IAH,IAP持续≥20mm Hg，

本文编号：1487096