微小巴贝斯虫烯醇化酶蛋白主要特征与抗原表位的生物信息学分析

发布时间：2018-02-03 11:17

  本文关键词： 微小巴贝斯虫 烯醇化酶 理化性质 抗原表位 生物信息学分析　出处：《中国病原生物学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的运用生物信息学方法分析微小巴贝斯虫烯醇化酶(BmEno)基因编码蛋白的主要特征和抗原表位。方法运用ORF Finder、PROSITE、ProtPar am、SignalP、TMpred、SOSUI、MotifScan、Bcepred、HNN等生物信息学在线分析软件,结合DNASTAR生物信息学分析软件,预测BmEno蛋白的开放阅读框、特征结构域、理化性质、信号肽、跨膜区、翻译后修饰位点、可溶性、亲水性、表面可及性、二级结构和抗原表位。结果该蛋白由438个氨基酸组成,在349-362位存在烯醇化酶的特征结构域,分子式为C_(2098)H_(3366)N_(570)O_(648)S_(18),分子质量单位为47.5203ku,等电点理论值为5.98,有25个翻译后修饰位点,10个亲水性参数得分≥1.9的区域,6个柔韧性参数得分≥2.0的区域,18个表面可及性参数得分≥1.9的区域,7个潜在的B细胞抗原表位,15个潜在的T细胞抗原表位,为可溶性蛋白。结论微小巴贝斯虫烯醇化酶蛋白为可溶性蛋白,有多个抗原表位,具有潜在的免疫原性,可以作为微小巴贝斯虫的疫苗候选抗原。
[Abstract]:Objective to analyze the main characteristics and antigenic epitopes of BmEnogene encoding protein by bioinformatics. Methods ORF Findern proside was used. The bioinformatics online analysis software, such as ProtPar signal Pnn TMpredl, motif Scann BcepredHNN and so on, is used to analyze bioinformatics on line. Combined with DNASTAR bioinformatics software, the open reading frame, characteristic domain, physicochemical properties, signal peptide, transmembrane region, post-translational modification site, soluble and hydrophilic of BmEno protein were predicted. Results the protein was composed of 438 amino acids and had the characteristic domain of enolase at 349-362. The molecular formula is C / T / 2098H / C / C / C / C / T / T / T / T / T / T / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C / C. There were 25 post-translational modification sites, 10 areas with hydrophilic parameter score 鈮，

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