微创技术下兔急性心房颤动模型的建立

发布时间：2018-03-08 18:13

本文选题：心房颤动　切入点：动物模型　出处：《实用心脑肺血管病杂志》2011年03期 　论文类型：期刊论文

【摘要】：目的探索在药物诱导下经食管高频刺激左心房建立兔急性心房颤动动物模型的效果及机制。方法 45只新西兰大耳白兔随机分为实验组(n=15)、对照组一(n=15)、对照组二(n=15)。对照组一经耳缘静脉持续泵入混合药物(2g/L氯乙酰胆碱56ml、去乙酰毛花苷2ml、异丙肾上腺素2ml),经食管置入电极(冠状窦10极电极)但不予Burst刺激。对照组二单纯经食管电极给予左心房Burst刺激。实验组通过耳缘静脉持续泵入混合药物,并经食管电极Burst刺激左心房建立兔急性房颤模型。根据心室率变化调整药物剂量,当心室率控制为基础心室率1/3时,经食管电极行S1S11000次/min的Burst刺激,观察房颤情况,将30min的房颤视为诱发成功。统计食管电极深度及诱发房颤时药物浓度,监测房颤发生情况、持续时间、房颤前后心室率及心房有效不应期(AERP)变化。结果实验组15只兔中11只成功诱发出持续时间30min的房颤,诱发率73%;两对照组均未诱发出房颤。房颤持续时间(44±10)min,分别与两对照组比较均有显著统计学意义(P0.01)。房颤时心室率(133±7)次/min明显快于Burst刺激诱发前(经静脉应用混合药物将心室率控制于基础心室率1/3,未行Burst刺激前)心室率(109±5)次/min,有显著统计学意义(P0.01)。房颤后AERP(75±8)ms明显短于基础(未应用药物时测定)AERP(116±8)ms,有显著统计学意义(P0.01)。诱发房颤混合药物剂量(18.6±1.7)ml/h,食管电极深度(16.8±0.5)cm。结论经静脉持续泵入混合药物(2g/L乙酰胆碱56ml、去乙酰毛花苷2ml、异丙肾上腺素2ml),并结合食管电极给予左心房Burst刺激方法构建兔急性房颤模型成本低、诱发率高、重复性好、创伤小、建模周期短、操作简单,运用微创技术建立了一种新的房颤模型的制作方法。
[Abstract]:Objective to explore the effect and mechanism of acute atrial fibrillation in rabbits induced by high frequency stimulation of left atrium via esophagus. Methods 45 New Zealand white rabbits were randomly divided into experimental group, control group and control group. Once the auricular vein was continuously pumped into the control group, the mixture of 2 g / L chloroacetylcholine 56 ml, deacetyl anthocyanin 2 ml, isoproterenol 2 ml was continuously pumped into the auricular vein, and the transesophageal implantation electrode (coronary sinus 10 pole electrode) was not stimulated by Burst. The control group 2 was simply transesophageal. The left atrium was stimulated with Burst at the electrode. The experimental group was continuously pumped into the auricular vein through the auricular vein. A rabbit model of acute atrial fibrillation was established by stimulation of left atrium with esophageal electrode Burst. The drug dose was adjusted according to the changes of ventricular rate. When the ventricular rate was controlled as the basic ventricular rate of 1/3, the esophageal electrode was given Burst stimulation of S1S1000times / min to observe atrial fibrillation. The incidence and duration of atrial fibrillation were monitored by counting the depth of esophageal electrode and the concentration of drugs during the induction of atrial fibrillation. Results the atrial fibrillation lasted 30 minutes in 11 of 15 rabbits in the experimental group. The atrial fibrillation duration was 44 卤10 min, which was significantly higher than that in the two control groups (P 0.01). The ventricular rate during atrial fibrillation was 133 卤7 times per minute faster than that before Burst stimulation (intravenous administration of mixed drugs). The ventricular rate was controlled at 1 / 3 of the basal ventricular rate before Burst stimulation. The ventricular rate was 109 卤5 times / min, which was significant (P 0.01). The AERP(75 卤8 Ms after atrial fibrillation was significantly shorter than that in the basal group (116 卤8 Ms) after atrial fibrillation, there was a significant difference (P 0.01) in inducing atrial fibrillation. The combined dose was 18.6 卤1.7 ml / h and the esophageal electrode depth was 16.8 卤0.5 cm 路cm.Conclusion continuous infusion of 2 g / L acetylcholine, 2 ml deacetylcholine, 2 ml isoproterenol 2 ml of acetylcholine, 2 ml of isoprenaline, combined with esophageal electrode stimulation of left atrium to construct rabbit model of acute atrial fibrillation is low in cost. It has the advantages of high induction rate, good repeatability, small trauma, short modeling period and simple operation. A new method of making atrial fibrillation model is established by using minimally invasive technique.
【作者单位】：福建医科大学教学医院(厦门大学附属中山医院心内科);
【分类号】：R541.7;R-332

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