抽动障碍大鼠模型的行为学特征及机制研究

发布时间：2018-03-10 10:51

本文选题：抽动障碍　切入点：动物模型　出处：《中国儿童保健杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的对亚氨基二丙腈(IDPN)和2,5-二甲氧-4-碘苯-2氨基丙烷(DOI)两种抽动障碍(TD)模型的行为学特征进行观察,并测定其血浆及纹状体中的多巴胺(DA)与5-羟色胺(5-HT)等神经递质水平,为更好地选择TD模型提供理论依据并揭示TD的发生机制。方法通过SD大鼠腹腔注射IDPN和DOI分别建立IDPN和DOI抽动障碍大鼠模型,应用双盲法观察记录两模型(大鼠)的行为学变化,从运动行为、刻板行为和分类刻板行为三个方面进行评估和比较。采用酶联免疫吸附试验(ELISA)检测两种模型鼠大脑纹状体和血浆中DA与5-HT等神经递质的含量,探讨IDPN与DOI动物模型的行为学特征及机制。结果 IDPN组大鼠和DOI组大鼠的运动行为评分和刻板行为评分均高于对照组,差异具有统计学意义(P0.05);IDPN组大鼠的旋转、舞蹈样运动高于对照组和DOI组,DOI组大鼠的口爪运动、自咬高于对照组和IDPN组,差异均具有统计学意义(P0.05)。IDPN组大鼠血浆及纹状体中的DA(5.70±3.12,137.45±20.14)明显高于对照组(0.32±0.12,68.13±12.34)和DOI组(1.01±0.74,88.56±21.30),差异具有统计学意义(F=13.43~8.6,P0.05)。DOI组大鼠纹状体中5-HT(56.83±34.72)明显低于对照组(109.14±14.05)和IDPN组(72.52±10.03),差异具有统计学意义(F=3.65,P0.05)。结论 IDPN模型主要表现为全身性抽动,DOI模型主要以局部抽动为主。IDPN模型可能通过影响DA系统,而DOI模型则可能通过激活5-HT受体系统,从而引起大鼠出现抽动症状。
[Abstract]:Objective to observe the behavioral characteristics of IDPN and 2-dimethoxy-4-iodobenzene-2-aminopropane (DOI) models, and to determine the levels of dopamine (DA) and serotonin (5-HT) in plasma and striatum. Methods SD rats were injected intraperitoneally with IDPN and DOI to establish IDPN and DOI dystic rat models, respectively, in order to provide theoretical basis for better selection of TD model and reveal the mechanism of TD. Two blind methods were used to observe and record the behavioral changes of the two models (rats). The levels of DA and 5-HT neurotransmitters in striatum and plasma of two kinds of model rats were measured by enzyme linked immunosorbent assay (Elisa). Results the scores of motor behavior and stereotypical behavior in IDPN group and DOI group were higher than those in control group, and the difference was statistically significant. Dance-like exercise was higher than that in control group and DOI group, and self-biting was higher than that in control group and IDPN group. The difference was statistically significant in plasma and striatum (DA(5.70 卤3.1277.45 卤20.14) of rats in P0.05 + IDPN group, 0.32 卤0.128.13 卤12.34 in control group and 1.01 卤0.74 卤88.56 卤21.30 in DOI group. The difference was statistically significant in F13.438.6P0.05P0.05.DOI group (56.83 卤34.72) compared with control group (109.14 卤14.05) and IDPN group (72.52 卤10.03g), and the difference was statistically significant compared with that in control group (109.14 卤14.05) and IDPN group (72.52 卤10.03). Conclusion the main manifestation of IDPN model is that the local twitch is the main model. IDPN model may influence DA system. The DOI model may activate 5-HT receptor system, which may induce tic symptoms in rats.
【作者单位】：青岛大学附属医院儿童保健科;烟台毓璜顶医院儿科;福建省立医院儿科;青岛大学附属医院遗传室;
【基金】：国家自然科学基金资助项目(81101017;81371499)
【分类号】：R749.94;R-332

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