Ghrelin对下丘脑PVN神经元放电活动的影响及其调控研究

发布时间：2018-03-16 04:35

本文选题：海马CA1区　切入点：PVN　出处：《青岛大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：观察电刺激海马CA1区对下丘脑室旁核(PVN)胃牵张(GD)敏感神经元放电活动的影响,以及ghrelin在这该通路中的调控作用。方法：采用细胞外记录神经元单位放电方法,观察ghrelin对大鼠下丘脑PVN内GD敏感神经元放电活动的影响及电刺激海马CA1区,PVN内GD敏感神经元自发放电活动的变化；同时给予ghrelin受体阻断剂[D-Lys-3]-GHRP-6,观察以上作用的受体机制。结果：在PVN记录到的109个GD敏感神经元中,有71个为GD兴奋性(GD-E)神经元,38个为GD抑制性(GD-I)神经元。在71个GD-E神经元中,微量注射ghrelin可兴奋其中72%的神经元,放电频率平均增加38.9±7.3%(t=2.85,P0.01,n=51)；而在38个GD-I神经元中,微量注射ghrelin可抑制其中60%的神经元,放电频率平均减少45.2±6.3%(t=3.08,P0.01,n=23)；ghrelin的效应可被ghrelin受体阻断剂[D-Lys-3]-GHRP-6阻断。在41个对ghrelin有兴奋反应的GD-E神经元和15个对ghrelin有抑制反应的GD-I神经元中,电刺激海马CA1区,可分别兴奋39%的GD-E和33%的GD-I神经元,其中44%的GD-E神经元的兴奋效应可被[D-Lys-3]-GHRP-6部分阻断。结论：海马CA1区可以调控PVN内GD敏感神经元的活性,ghrelin能神经纤维参与了该通路的调控。
[Abstract]:Aim: to observe the effect of electrical stimulation of hippocampal CA1 on the activity of gastric distraction neurons in hypothalamic paraventricular nucleus (PVN) and the regulation of ghrelin in this pathway. Methods: the effects of ghrelin on the activity of GD sensitive neurons in hypothalamus PVN and the changes of spontaneous discharges of GD sensitive neurons in hippocampal CA1 region were observed by extracellular recording of neuron unit discharges. At the same time, ghrelin receptor blocker [D-Lys- 3] -GHRP-6 was given to observe the receptor mechanism. Results: of the 109 GD sensitive neurons recorded by PVN, 71 were GD excitatory GD-E neurons and 38 were GD inhibitory GD-I- neurons. Of the 71 GD-E neurons, 72% were excited by microinjection of ghrelin. The average frequency of discharge increased by 38.9 卤7.3%, and 60% of the 38 GD-I neurons were inhibited by microinjection of ghrelin. The effect of ghrelin receptor blocker [D-Lys-3] -GHRP-6 on the average reduction of discharge frequency was 45.2 卤6.3.The effect of 3.08p 0.01p0.01hrelin was blocked by ghrelin receptor antagonist [D-Lys-3] -GHRP-6. In 41 GD-E neurons that responded to ghrelin and 15 GD-I neurons that inhibited ghrelin, the hippocampal CA1 region was stimulated by electrical stimulation. 39% GD-E and 33% GD-I neurons could be excited, and 44%% of GD-E neurons could be partially blocked by [D-Lys-3] -GHRP-6. Conclusion: hippocampal CA1 can regulate the activity of GD sensitive neurons in PVN.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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