改良左旋支结扎法微创小型猪急性心肌梗死模型建立与评估

发布时间：2018-04-01 17:12

本文选题：急性心肌梗死　切入点：小型猪　出处：《中国实验动物学报》2017年04期

【摘要】：目的应用改良的左冠状动脉回旋支结扎方法联合微创手术,制备小型猪急性心肌梗死模型,评估其有效性和稳定性,并探讨其在科研应用中的意义。方法选择3月龄巴马香猪25头,麻醉下行气管插管呼吸机辅助通气,微创开胸结扎左旋支中段,建立急性心肌梗死模型。并于术前、术后1 h、4周行心脏超声评估模型动物的心脏功能,术后4周处死动物,取心脏行TTC染色评估梗死面积及病理变化,统计死亡率和死亡原因。结果结扎术后1 h和4周后心脏功能较术前均明显降低,EF值由(64.2±4.6)%分别降至(48.2±5.3)%和(49.7±6.1)%(P0.01),左心室侧壁室壁厚度变薄,胶原增生。梗死后1 h室颤率17.3%,梗死面积为(19.2±3)%。结论应用改良的左冠状动脉回旋支结扎方法联合微创手术能够建立稳定的猪心肌梗死模型,具有手术创伤小、模型稳定和死亡率低等特点,为相关研究提供了经济、理想的动物模型。
[Abstract]:Objective to establish a mini-pig model of acute myocardial infarction by modified left coronary artery circumflex branch ligation combined with minimally invasive surgery, and to evaluate its efficacy and stability. Methods 25 Bama pigs aged 3 months were selected for tracheal intubation and ventilator assisted ventilation under anesthesia. The model of acute myocardial infarction was established by minimally invasive thoracotomy and ligation of left circumflex branch. The cardiac function of the model animals was evaluated by echocardiography at 1 hour and 4 weeks after operation, and the animals were killed 4 weeks after operation. The infarct size and pathological changes were evaluated by TTC staining. Results after 1 h and 4 weeks after ligation, the cardiac function decreased significantly from 64.2 卤4.6% to 48.2 卤5.3% and 49.7 卤6.1%, respectively, and the left ventricular wall thickness became thinner. Collagen proliferation. The ventricular fibrillation rate was 17.3g / h after infarction, and the infarct area was 19.2 卤30.Conclusion the modified left coronary circumflex branch ligation combined with minimally invasive surgery can establish a stable model of pig myocardial infarction with minimal surgical trauma. The characteristics of stable model and low mortality provide economic and ideal animal model for related research.
【作者单位】：同济大学附属东方医院转化医学中心;同济大学附属东方医院心衰专科;同济大学附属东方医院心脏超声科;
【基金】：国家自然科学基金(编号:81470393) 上海市领军人才(编号:2012053) 上海市浦东新区卫生系统优秀青年医学人才培养计划(编号:PWRq2014-01)
【分类号】：R-332;R542.22

【相似文献】