神经肽S在小鼠福尔马林实验中的镇痛活性研究

发布时间：2018-04-21 08:00

本文选题：神经肽S + (NPS)　；参考：《兰州大学》2011年硕士论文

【摘要】：神经肽S (neuropeptide S, NPS)是最近发现的生物活性肽,具有调节睡眠与觉醒、情绪、运动、摄食、学习和记忆、药物成瘾等方面的作用。NPS的受体(NPSR)分布于痛觉下行抑制系统相关的脑区,例如中脑导水管周围灰质(periaqueductal gray, PAG),这提示着NPS在痛觉调节方面的作用。在我们的实验中,使用小鼠福尔马林实验首次评价了NPS对炎症痛的调节作用。NPS (0.1-100 pmol, i.c.v.)能够剂量依赖的削弱小鼠脚掌注射福尔马林引起的两相痛觉行为。侧脑室共同注射NPS (10 pmol)和NPSR的肽类拮抗剂[D-Val5]NPS (1000和10000 pmo1),能够拮抗NPS在该模型中的镇痛作用,而单独给予相同剂量的[D-Val5]NPS则既不引起镇痛反应也不引起痛敏反应。在相同的实验条件下,腹腔注射纳络酮(10mg/kg体重)并不影响NPS(10 pmol, i.c.v.)产生的镇痛作用,但却削弱了吗啡(1000 pmol,i.c.v.)引起的镇痛作用。结果还显示,在脚掌注射了福尔马林的小鼠中,与生理盐水组(i.c.v.)相比,NPS (10 pmol, i.c.v.)几乎增加了PAG中被检测的所有亚区的c-fos蛋白的表达量。这些结果表明,NPS对炎症痛的抑制作用,是通过NPS受体而不是阿片受体所介导的,可能涉及到PAG的激活,这提示着NPS系统可能是发展新的镇痛药的潜在靶点。
[Abstract]:Neuropeptide S (NPS) is a recently discovered bioactive peptide that regulates sleep and arousal, moods, motility, ingestion, learning and memory, drug addiction, and so on. Periaqueductal grays, for example, suggest the role of NPS in the regulation of pain. In our experiment, the mouse formalin experiment was used to evaluate the effect of NPS on inflammatory pain for the first time. The mice were able to attenuate the two-phase pain induced by formalin injection in a dose-dependent manner. Intracerebroventricular injection of NPS (10 pmol) and NPSR's peptide antagonists [D-Val5] NPS 1000 and 10000 pmol ~ (-1) could antagonize the analgesic effect of NPS in the model, while the same dose of [D-Val5] NPS alone did not induce analgesic or pain-sensitive response. Under the same experimental conditions, intraperitoneal injection of naloxone (10 mg / kg BW) did not affect NPS(10 pmol. i.c.v.) The analgesic effect was reduced by 1 000 pmol. i.c.v.) An analgesic effect. The results also showed that in mice injected with formalin on the soles of feet, the mice were treated with normal saline group (i.c.v.) NPS 10 pmol, i.c.v.) It increased the expression of c-fos protein in almost all subregions detected in PAG. These results suggest that the inhibition of inflammatory pain is mediated by NPS receptors rather than opioid receptors and may involve the activation of PAG suggesting that the NPS system may be a potential target for the development of new analgesics.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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