应用两种不同建模方式构建子痫前期SD大鼠动物模型的实验研究

发布时间：2018-04-21 16:24

本文选题：建模 + 构建　；参考：《实用妇产科杂志》2017年09期

【摘要】：目的:探讨分析应用两种不同建模方式来构建子痫前期SD大鼠的动物模型的特异性。方法:SD孕鼠随机均分3组,空白对照组同前饲养,合体滋养细胞微绒毛(STBM)组用STBM诱导,STBM+亚硝基左旋精氨酸甲酯(L-NAME)组用STBM+L-NAME诱导。检测蛋白尿(U-TP)、血压(BP)、部分凝血活酶时间(APTT)、凝血酶原时间(PT)和D-二聚体(D-D),HE染色胎盘组织,Real time PCR和Western blot测胎盘组织可溶性血管内皮生长因子受体-1(s Flt-1)、可溶性内皮因子(SEng)、胎盘生长因子(PLGF)mRNA及蛋白表达,滋养细胞分离、培养后行功能学实验(EDU测增殖、FCM测凋亡、Transwell测侵袭)。结果:U-TP、BP、APTT、PT及D-D在孕10天3组比较差异无统计学意义(P0.05),孕15天及20天U-TP、BP STBM+L-NAME组较STBM组显著增加(P0.05),孕20天APTT、PT STBM+L-NAME组较STBM组显著缩短,D-D显著增加,空白对照组无明显变化。STBM+L-NAME组HE染色见胎盘蜕膜带水肿明显,炎性细胞浸润,基带和迷路带见滋养细胞增生。3组比较s Flt-1、SEng、PLGF mRNA及蛋白表达差异有统计学意义(P0.05)。STBM+L-NAME组s Flt-1、SEng mRNA及蛋白比STBM组明显增高(P0.05),PLGF mRNA及蛋白明显减低(P0.05)。功能学实验中3组细胞增殖和凋亡差异均有统计学意义(P0.05)。STBM+L-NAME组滋养细胞增殖和侵袭最少,凋亡最多。与STBM组比较差异有统计学意义(P0.05)。结论:在构建子痫前期动物模型中,STBM合用L-NAME诱导比单独应用STBM特异性更强,为临床子痫前期诊疗提供可靠模式研究依据。
[Abstract]:Aim: to investigate the specificity of two different modeling methods in constructing preeclampsia SD rat model. Methods Twenty SD pregnant rats were randomly divided into 3 groups. The control group was fed with STBM L-NAME. The syncytiotrophoblast microvillus chorionic chorionic chorionic microvilli (STBM) group was induced by STBM L-NAME. Detection of proteinuria (UTP), blood pressure (BP), partial thromboplastin time (APTTT), prothrombin time (PTT) and D- dimer (D-DX) HE staining of placental tissue Real time PCR and Western blot for the detection of soluble vascular endothelial growth factor receptor -1s Flt-1, soluble endothelial factor (et) The mRNA and protein expression of placental growth factor-PLGFN were observed. Cultured trophoblastic cells were separated and cultured. EDU was used to measure proliferation and FCM to detect apoptosis and Transwell to detect invasion. Results there was no significant difference in PT and D-D between the three groups on the 10th day of gestation. On the 15th and 20th day of gestation, the levels of P0.05 and D-D in the 15 and 20 days of gestation group were significantly higher than those in the STBM group, and the D-D in the STBM L-NAME group was significantly shorter than that in the STBM group on the 20th day of gestation. There was no obvious change in the blank control group. In the STBM L-NAME group, he staining showed that the placental decidua was edema and inflammatory cell infiltration. There were significant differences in the expression of mRNA and protein between the basal zone group and the labyrinthine zone group. There was a significant difference in the expression of mRNA and protein between the two groups. Compared with the STBM group, the expression of sFlt-1 SEng mRNA and protein in the sFlt-1 L-NAME group was significantly higher than that in the STBM group. The proliferation and apoptosis of trophoblastic cells in the three groups were significantly different from those in the control group (P 0.05). STBM L-NAME group had the least proliferation and invasion and the most apoptosis. Compared with STBM group, the difference was statistically significant (P 0.05). Conclusion: STBM combined with L-NAME is more specific than STBM alone in constructing animal model of preeclampsia, which provides a reliable basis for clinical diagnosis and treatment of preeclampsia.
【作者单位】：南方医科大学附属花都医院;
【基金】：广东省科技厅科技项目(编号:2015A030302004,2015A030302003) 广东省中医药局科技项目(编号:20161191,20151061) 广东省自然科学基金(编号:2016A030313419)
【分类号】：R-332;R714.244

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