白藜芦醇对慢性哮喘小鼠模型气道炎症的影响

发布时间：2018-04-28 15:56

本文选题：过敏性哮喘 + 白细胞介素-　；参考：《中国临床药理学杂志》2017年01期

【摘要】：目的研究白藜芦醇对小鼠慢性哮喘的作用。方法小鼠鼻内滴注螨虫(HDM)粗提物25μg,每周5次,连续7周,制备小鼠过敏性哮喘模型。将哮喘小鼠随机分为实验组(白藜芦醇100 mg·kg~(-1)),对照组(地塞米松1 mg·kg~(-1)),另设正常组及模型组(均0.9%Na Cl),每组15只。2周后,称量小鼠体重,以酶联免疫吸附法测定小鼠肺灌流液(BALF)中细胞因子含量;观察小鼠肺组织的病理学变化。结果在小鼠的BALF中,模型组的白细胞介素-17(IL-17)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、肿瘤生长因子-β(TGF-β)分别为(186.0±101.0)pg·mL~(-1),(29.6±10.6)ng·mL~(-1),(11.1±3.2)ng·mL~(-1),(158.5±10.0)ng·mL~(-1)。实验组的IL-17、IL-6、TNF-α、TGF-β分别为(43.1±10.3)pg·mL~(-1),(10.4±1.4)ng·mL~(-1),(5.0±1.7)ng·mL~(-1),(107.8±17.3)ng·mL~(-1)。与模型组相比,白藜芦醇能明显抑制BALF中IL-17、IL-6、TNF-α、TGF-β的表达,差异有统计学意义(P0.05)。实验组对小鼠体重的改变没有影响。结论白藜芦醇对螨虫诱导的小鼠慢性哮喘具有一定的改善作用。
[Abstract]:Objective to study the effect of resveratrol on chronic asthma in mice. Methods mice allergic asthma model was established by intranasal infusion of 25 渭 g crude extract of HDM, 5 times a week for 7 weeks. The asthmatic mice were randomly divided into experimental group (100 mg / kg resveratrol) and control group (dexamethasone 1 mg / kg). The mice were divided into two groups: normal group and model group. The levels of cytokines in mouse lung perfusion fluid (BALF) were determined by enzyme-linked immunosorbent assay (Elisa), and the pathological changes of lung tissue were observed. Results in BALF of mice, the levels of IL-17, IL-6, TNF- 伪, TGF- 尾 in the model group were 186.0 卤101.0)pg mLL- 1 and 29.6 卤10.6)ng mLL-1, respectively. The levels of IL-17, IL-6, TNF- 伪 and TGF- 尾 in the model group were 11.1 卤3.2)ng mLL-1, 158.5 卤10.0)ng mLL-1, respectively. The levels of IL-17, IL-6, TNF- 伪 and TGF- 尾 in the experimental group were 43.1 卤10.3)pg mLL, 10.4 卤1.4)ng mLL, 5. 0 卤1.7)ng mLL, and 107.8 卤17.3)ng mLmLTF- 1, respectively. Compared with the model group, resveratrol could significantly inhibit the expression of IL-17, IL-6, TNF- 伪 and TGF- 尾 in BALF, and the difference was statistically significant (P 0.05). The experimental group had no effect on the weight change of mice. Conclusion resveratrol can improve chronic asthma induced by mites in mice.
【作者单位】：江西医学高等专科学校;南昌大学医学院;
【基金】：江西省教育厅青年基金资助项目(14226)
【分类号】：R285.5;R-332

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