香烟烟雾法肺动脉高压小鼠模型肺组织HIF-1α和Nrf2表达

发布时间：2018-05-02 03:23

本文选题：肺动脉高压 + 吸烟　；参考：《江西师范大学学报(自然科学版)》2017年02期

【摘要】：以香烟烟雾熏烟法建立肺动脉高压(PAH)小鼠模型,将C57B6J小鼠随机分为对照组和模型组,每组10只,对照组小鼠不作任何处理,模型组采用香烟处理:每天2次,每次2 h,10支/h,6 d/周,连续6个月的建模方法;使用小鼠右心室压力测定仪(BIOPAC Systems,MP150)测定小鼠右心室压力(RVSP);将肺小动脉进行胶原纤维染色(Van-Gieson法),结合Image Pro Plus软件分析,测量肺小动脉血管的中层壁厚和肺小动脉血管的外径,计算肺小动脉厚度的变化;利用Western Blot法检测Nrf2和HIF-1α蛋白在肺组织的表达.结果表明:1)与对照组相比,模型组小鼠右心室压力明显增加,肺小动脉血管壁明显增厚,管腔变狭窄,右心室与体重的比值显著增加;2)与对照组相比,模型组小鼠肺组织HIF-1α蛋白表达明显增加,而Nrf2蛋白表达明显减少.研究结果意味着:HIF-1α与Nrf2在香烟烟雾法建立的肺动脉高压小鼠肺组织中表达异常.
[Abstract]:The C57B6J mice were randomly divided into control group and model group with 10 rats in each group. The control group did not do any treatment, and the model group was treated with cigarette twice a day. A 6-month modeling method was used to measure the right ventricular pressure in mice by BIOPAC Systems / MP150. The pulmonary arterioles were stained with collagen fibers and analyzed with Image Pro Plus software. The medial wall thickness of pulmonary arterioles and the external diameter of pulmonary arterioles were measured, and the changes of pulmonary arterioles thickness were calculated, and the expression of Nrf2 and HIF-1 伪 protein in lung tissue was detected by Western Blot method. The results showed that compared with the control group, the right ventricular pressure, pulmonary arteriole wall thickening, lumen stenosis and the ratio of right ventricle to body weight were significantly increased in the model group compared with the control group. In the model group, the expression of HIF-1 伪 protein increased significantly, but the expression of Nrf2 protein decreased significantly. The results indicated that the expression of 1: HIF-1 伪 and Nrf2 was abnormal in lung tissues of pulmonary hypertension mice established by cigarette smoke method.
【作者单位】：北京大学深圳研究生院化学院;广州医科大学附属第一医院研究所广州呼吸疾病研究所;中山大学第八附属医院;
【基金】：国家自然科学基金(30971166) 深圳市科技计划基础研究(JCYJ20140416094256880)资助项目
【分类号】：R-332;R544.1

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