小鼠肾脏移植模型的建模

发布时间：2018-05-07 06:18

本文选题：小鼠 + 肾脏移植　；参考：《浙江大学》2011年硕士论文

【摘要】：目的在借鉴国内外以及前人的手术技术的经验基础上,本人通过不断摸索和大量的练习,建立了一种更简便快捷稳定的小鼠肾移植模型。实验方法采用C57BL/6小鼠行25例同种异体异位肾移植,将供体带有肾动脉的一段腹主动脉与受体腹主动脉行端侧吻合；供体的肾静脉与受体的下腔静脉行端侧吻合。输尿管重建采用改良法。结果小鼠肾脏移植模型成功率可达到84%。供体手术时间(12±2)min,受体手术血管准备时间(6±2)min,动静脉血管吻合(20±3)min,输尿管吻合时间(5±1min)。总手术时间为55±5min。另外切除了10只C57BL/6小鼠的双肾观察其生存时间,以便对照移植生存率。结论该模型简单快捷,成功率高,是理想的手术模型。
[Abstract]:Purpose On the basis of the experience of domestic and foreign and previous surgical techniques, I have established a more convenient and stable mouse kidney transplantation model through continuous exploration and a lot of practice. Experimental method Twenty-five cases of allogenic heterotopic kidney transplantation were performed in C57BL/6 mice. A segment of the donor aorta with renal artery was end-to-side anastomosed with the recipient abdominal aorta, and the donor renal vein was end-to-side anastomosed with the recipient inferior vena cava. Ureteral reconstruction was improved. Result The success rate of kidney transplantation in mice was 84%. The donor operation time was 12 卤2 minutes, the recipient vessel preparation time was 6 卤2 minutes, the arteriovenous anastomosis time was 20 卤3 minutes, and the ureteral anastomosis time was 5 卤1 min. The total operative time was 55 卤5 min. In addition, the survival time of 10 C57BL/6 mice was observed in order to control the survival rate. Conclusion The model is simple and fast, and has high success rate. It is an ideal surgical model.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R699.2

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